Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus
Before seawater transfer, farmed Atlantic salmon are subjected to treatments that may affect the immune system and susceptibility to pathogens. E.g., exposure to constant light (CL) stimulates smoltification, which prepares salmon to life in sea water, but endocrine changes in this period are associ...
Published in: | Fish & Shellfish Immunology |
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Online Access: | https://hdl.handle.net/11250/3006344 https://doi.org/10.1016/j.fsi.2021.07.002 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/3006344 2023-05-15T15:32:12+02:00 Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus Bakke, Anne Flore Rebl, Alexander Frost, Petter Afanasyev, Sergey Røyset, Kristoffer Alnes Søfteland, Tina Lund, Hege Boysen, Preben Krasnov, Aleksei 2021-12-07T15:45:13Z application/pdf https://hdl.handle.net/11250/3006344 https://doi.org/10.1016/j.fsi.2021.07.002 eng eng Norges forskningsråd: 267644 Fish and Shellfish Immunology. 2021, 118 188-196. urn:issn:1050-4648 https://hdl.handle.net/11250/3006344 https://doi.org/10.1016/j.fsi.2021.07.002 cristin:1965746 188-196 118 Fish and Shellfish Immunology Peer reviewed Journal article 2021 ftunivmob https://doi.org/10.1016/j.fsi.2021.07.002 2022-07-20T22:41:11Z Before seawater transfer, farmed Atlantic salmon are subjected to treatments that may affect the immune system and susceptibility to pathogens. E.g., exposure to constant light (CL) stimulates smoltification, which prepares salmon to life in sea water, but endocrine changes in this period are associated with suppression of immune genes. Salmon are vaccinated towards end of the freshwater period to safeguard that adequate vaccine efficacy is achieved by the time the fish is transferred to sea. In the present study, we investigated how the responses to vaccination and viral infection varied depending on the time of CL onset relative to vaccination. The salmon were either exposed to CL two weeks prior to vaccination (2-PRI) or exposed to CL at the time of vaccination (0-PRI). A cohabitant challenge with salmonid alphavirus, the causative agent of pancreatic disease, was performed 9 weeks post vaccination. The immunological effects of the different light manipulation were examined at 0- and 6-weeks post vaccination, and 6 weeks post challenge. Antibody levels in serum were measured using a serological bead-based multiplex panel as well as ELISA, and 92 immune genes in heart and spleen were measured using an integrated fluidic circuit-based qPCR array for multiple gene expression. The 2-PRI group showed a moderate transcript down-regulation of genes in the heart at the time of vaccination, which were restored 6 weeks after vaccination (WPV). Conversely, at 6WPV a down-regulation was seen for the 0-PRI fish. Moreover, the 2-PRI group had significantly higher levels of antibodies binding to three of the vaccine components at 6WPV, compared to 0-PRI. In response to SAV challenge, transcription of immune genes between 2-PRI and 0-PRI was markedly dissimilar in the heart and spleen of control fish, but no difference was found between vaccinated salmon from the two CL regimens. Thus, by using labor-saving high throughput detection methods, we demonstrated that light regimens affected antibody production and transcription ... Article in Journal/Newspaper Atlantic salmon Open archive Norwegian University of Life Sciences: Brage NMBU Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Fish & Shellfish Immunology 118 188 196 |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
description |
Before seawater transfer, farmed Atlantic salmon are subjected to treatments that may affect the immune system and susceptibility to pathogens. E.g., exposure to constant light (CL) stimulates smoltification, which prepares salmon to life in sea water, but endocrine changes in this period are associated with suppression of immune genes. Salmon are vaccinated towards end of the freshwater period to safeguard that adequate vaccine efficacy is achieved by the time the fish is transferred to sea. In the present study, we investigated how the responses to vaccination and viral infection varied depending on the time of CL onset relative to vaccination. The salmon were either exposed to CL two weeks prior to vaccination (2-PRI) or exposed to CL at the time of vaccination (0-PRI). A cohabitant challenge with salmonid alphavirus, the causative agent of pancreatic disease, was performed 9 weeks post vaccination. The immunological effects of the different light manipulation were examined at 0- and 6-weeks post vaccination, and 6 weeks post challenge. Antibody levels in serum were measured using a serological bead-based multiplex panel as well as ELISA, and 92 immune genes in heart and spleen were measured using an integrated fluidic circuit-based qPCR array for multiple gene expression. The 2-PRI group showed a moderate transcript down-regulation of genes in the heart at the time of vaccination, which were restored 6 weeks after vaccination (WPV). Conversely, at 6WPV a down-regulation was seen for the 0-PRI fish. Moreover, the 2-PRI group had significantly higher levels of antibodies binding to three of the vaccine components at 6WPV, compared to 0-PRI. In response to SAV challenge, transcription of immune genes between 2-PRI and 0-PRI was markedly dissimilar in the heart and spleen of control fish, but no difference was found between vaccinated salmon from the two CL regimens. Thus, by using labor-saving high throughput detection methods, we demonstrated that light regimens affected antibody production and transcription ... |
format |
Article in Journal/Newspaper |
author |
Bakke, Anne Flore Rebl, Alexander Frost, Petter Afanasyev, Sergey Røyset, Kristoffer Alnes Søfteland, Tina Lund, Hege Boysen, Preben Krasnov, Aleksei |
spellingShingle |
Bakke, Anne Flore Rebl, Alexander Frost, Petter Afanasyev, Sergey Røyset, Kristoffer Alnes Søfteland, Tina Lund, Hege Boysen, Preben Krasnov, Aleksei Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
author_facet |
Bakke, Anne Flore Rebl, Alexander Frost, Petter Afanasyev, Sergey Røyset, Kristoffer Alnes Søfteland, Tina Lund, Hege Boysen, Preben Krasnov, Aleksei |
author_sort |
Bakke, Anne Flore |
title |
Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
title_short |
Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
title_full |
Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
title_fullStr |
Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
title_full_unstemmed |
Effect of two constant light regimens on antibody profiles and immune gene expression in Atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
title_sort |
effect of two constant light regimens on antibody profiles and immune gene expression in atlantic salmon following vaccination and experimental challenge with salmonid alphavirus |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/3006344 https://doi.org/10.1016/j.fsi.2021.07.002 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
188-196 118 Fish and Shellfish Immunology |
op_relation |
Norges forskningsråd: 267644 Fish and Shellfish Immunology. 2021, 118 188-196. urn:issn:1050-4648 https://hdl.handle.net/11250/3006344 https://doi.org/10.1016/j.fsi.2021.07.002 cristin:1965746 |
op_doi |
https://doi.org/10.1016/j.fsi.2021.07.002 |
container_title |
Fish & Shellfish Immunology |
container_volume |
118 |
container_start_page |
188 |
op_container_end_page |
196 |
_version_ |
1766362706357518336 |