Comparative molecular characterization of novel and known piscine toti-like viruses
Totiviridae is a virus family well known to infect uni-cellular organisms like fungi and protozoa. In more recent years, viruses characterized as toti-like viruses, have been found in primarily arthropods, but also a couple in planarians and piscine species. These toti-like viruses share phylogeneti...
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ftunivmob:oai:nmbu.brage.unit.no:11250/2786574 2023-05-15T15:32:50+02:00 Comparative molecular characterization of novel and known piscine toti-like viruses Sandlund, Liv Mor, Sunil K. Singh, Vikash K. Padhi, Soumesh K. Phelps, Nicholas B. D. Nylund, Stian Mikalsen, Aase Beathe 2021-07-07T10:35:20Z application/pdf https://hdl.handle.net/11250/2786574 https://doi.org/10.3390/v13061063 eng eng Norges forskningsråd: 301083 urn:issn:1999-4915 https://hdl.handle.net/11250/2786574 https://doi.org/10.3390/v13061063 cristin:1920626 13 Viruses 6 Peer reviewed Journal article 2021 ftunivmob https://doi.org/10.3390/v13061063 2021-10-06T22:37:24Z Totiviridae is a virus family well known to infect uni-cellular organisms like fungi and protozoa. In more recent years, viruses characterized as toti-like viruses, have been found in primarily arthropods, but also a couple in planarians and piscine species. These toti-like viruses share phylogenetic similarities to totiviruses; however, their genomes also includes additional coding sequences in either 5′ or 3′ ends expected to relate to more advanced infection mechanisms in more advanced hosts. Here, we applied next generation sequencing (NGS) technologies and discovered three new toti-like viruses, one in wild common carp and one in bluegill from the USA and one in farmed lumpsucker from Norway. These are named common carp toti-like virus 1 (CCTLV-1), bluegill toti-like virus 1 (BGTLV-1), and Cyclopterus lumpus toti-like virus (CLuTLV), respectively. The genomes of these viruses have been characterized and compared to the three previously known piscine toti-like viruses, piscine myocarditis virus (PMCV) found in Atlantic salmon and the two from golden shiner, now named golden shiner toti-like virus 1 and 2 (GSTLV-1 and -2), and also to totiviruses and other toti-like viruses. We found that four piscine toti-like viruses had additional gene(s) in the 3′ end of the genome, and also clustered phylogenetically based on both capsid and RdRp-genes. This cluster constituted a distant branch in the Totiviridae, and we suggest this should be defined as a separate genus named Pistolvirus, to reflect this major cluster of piscine toti-like viruses. The remaining two piscine toti-like viruses differentiated from these by lacking any additional 3′ end genes and also by phylogenetical relation, but were both clustering with arthropod viruses in two different clusters. publishedVersion Article in Journal/Newspaper Atlantic salmon Open archive Norwegian University of Life Sciences: Brage NMBU Norway Viruses 13 6 1063 |
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Open Polar |
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Open archive Norwegian University of Life Sciences: Brage NMBU |
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ftunivmob |
language |
English |
description |
Totiviridae is a virus family well known to infect uni-cellular organisms like fungi and protozoa. In more recent years, viruses characterized as toti-like viruses, have been found in primarily arthropods, but also a couple in planarians and piscine species. These toti-like viruses share phylogenetic similarities to totiviruses; however, their genomes also includes additional coding sequences in either 5′ or 3′ ends expected to relate to more advanced infection mechanisms in more advanced hosts. Here, we applied next generation sequencing (NGS) technologies and discovered three new toti-like viruses, one in wild common carp and one in bluegill from the USA and one in farmed lumpsucker from Norway. These are named common carp toti-like virus 1 (CCTLV-1), bluegill toti-like virus 1 (BGTLV-1), and Cyclopterus lumpus toti-like virus (CLuTLV), respectively. The genomes of these viruses have been characterized and compared to the three previously known piscine toti-like viruses, piscine myocarditis virus (PMCV) found in Atlantic salmon and the two from golden shiner, now named golden shiner toti-like virus 1 and 2 (GSTLV-1 and -2), and also to totiviruses and other toti-like viruses. We found that four piscine toti-like viruses had additional gene(s) in the 3′ end of the genome, and also clustered phylogenetically based on both capsid and RdRp-genes. This cluster constituted a distant branch in the Totiviridae, and we suggest this should be defined as a separate genus named Pistolvirus, to reflect this major cluster of piscine toti-like viruses. The remaining two piscine toti-like viruses differentiated from these by lacking any additional 3′ end genes and also by phylogenetical relation, but were both clustering with arthropod viruses in two different clusters. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Sandlund, Liv Mor, Sunil K. Singh, Vikash K. Padhi, Soumesh K. Phelps, Nicholas B. D. Nylund, Stian Mikalsen, Aase Beathe |
spellingShingle |
Sandlund, Liv Mor, Sunil K. Singh, Vikash K. Padhi, Soumesh K. Phelps, Nicholas B. D. Nylund, Stian Mikalsen, Aase Beathe Comparative molecular characterization of novel and known piscine toti-like viruses |
author_facet |
Sandlund, Liv Mor, Sunil K. Singh, Vikash K. Padhi, Soumesh K. Phelps, Nicholas B. D. Nylund, Stian Mikalsen, Aase Beathe |
author_sort |
Sandlund, Liv |
title |
Comparative molecular characterization of novel and known piscine toti-like viruses |
title_short |
Comparative molecular characterization of novel and known piscine toti-like viruses |
title_full |
Comparative molecular characterization of novel and known piscine toti-like viruses |
title_fullStr |
Comparative molecular characterization of novel and known piscine toti-like viruses |
title_full_unstemmed |
Comparative molecular characterization of novel and known piscine toti-like viruses |
title_sort |
comparative molecular characterization of novel and known piscine toti-like viruses |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2786574 https://doi.org/10.3390/v13061063 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
13 Viruses 6 |
op_relation |
Norges forskningsråd: 301083 urn:issn:1999-4915 https://hdl.handle.net/11250/2786574 https://doi.org/10.3390/v13061063 cristin:1920626 |
op_doi |
https://doi.org/10.3390/v13061063 |
container_title |
Viruses |
container_volume |
13 |
container_issue |
6 |
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1063 |
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1766363313191518208 |