Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water
Estuarine environments have a high risk of metal pollution due to the influx from rivers. Freshwater from rivers mixed with seawater in coastal areas gives varied and complex water chemistry. In the estuarine environment of Kaldvellfjorden in Norway were the metals copper (Cu), zinc (Zn) and aluminu...
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Norwegian University of Life Sciences, Ås
2020
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ftunivmob:oai:nmbu.brage.unit.no:11250/2724830 2023-07-16T03:57:31+02:00 Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water Nymo, Malene Therese Teien, Hans-Christian Skipperud, Lindis Jaroz, Emil Norway 2020 application/pdf https://hdl.handle.net/11250/2724830 eng eng Norwegian University of Life Sciences, Ås Norwegian Research Council NRC: 268294 https://hdl.handle.net/11250/2724830 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no 96 Uptake Speciation Trace metals Cu Zn Al Fish Salmon Coastal Brackish Master thesis 2020 ftunivmob 2023-06-28T22:47:18Z Estuarine environments have a high risk of metal pollution due to the influx from rivers. Freshwater from rivers mixed with seawater in coastal areas gives varied and complex water chemistry. In the estuarine environment of Kaldvellfjorden in Norway were the metals copper (Cu), zinc (Zn) and aluminum (Al) classified to have a high risk of impact. If these metals are bioavailable, based on their speciation, fish can accumulate the metals in tissue. To improve the knowledge of bioavailability and toxicity of metals in coastal waters are the main objectives of this thesis to identify the uptake of trace metals in fish in coastal water. Atlantic salmon smolts (Salmo salar) were exposed to waterborne Cu, Zn, or Al in brackish water (20‰, pH 8) for 96-hours. The exposures were conducted with nine concentrations of Cu and Zn, and six concentrations of Al. Several of the concentrations were the same, to directly compare the uptake between the metals. To investigate the uptake in fish; gills, kidneys, and livers were sampled at the end of the exposure. Metal concentrations were quantified using Inductively Coupled Plasma Mass spectrometry (ICP-MS). Water samples of the exposure water were size- and charge- fractionated in-situ at 0h and 96h, and analyzed later using ICP-MS. The results of size fractionation revealed that speciation of the Cu, Zn, and Al was relatively similar in brackish water, and was assumed to be bioavailable. Aluminum was found associated with the low molecular mass (LMM, <10kDa) fraction at >83% of the total, LMM-associated Zn at >78% of the total, while Cu had large uncertainties in this fraction. Copper was either associated with the colloidal or LMM fraction. Only a small percentage, <14%, of the metals were associated with particles. This low association is likely due to low organic content in the exposure water. The uptake of metals on fish followed the order, Al>Cu>Zn, where gills were the tissue with the highest uptake, kidneys intermediate and the liver had no uptake within ... Master Thesis Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Norway |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
topic |
Uptake Speciation Trace metals Cu Zn Al Fish Salmon Coastal Brackish |
spellingShingle |
Uptake Speciation Trace metals Cu Zn Al Fish Salmon Coastal Brackish Nymo, Malene Therese Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
topic_facet |
Uptake Speciation Trace metals Cu Zn Al Fish Salmon Coastal Brackish |
description |
Estuarine environments have a high risk of metal pollution due to the influx from rivers. Freshwater from rivers mixed with seawater in coastal areas gives varied and complex water chemistry. In the estuarine environment of Kaldvellfjorden in Norway were the metals copper (Cu), zinc (Zn) and aluminum (Al) classified to have a high risk of impact. If these metals are bioavailable, based on their speciation, fish can accumulate the metals in tissue. To improve the knowledge of bioavailability and toxicity of metals in coastal waters are the main objectives of this thesis to identify the uptake of trace metals in fish in coastal water. Atlantic salmon smolts (Salmo salar) were exposed to waterborne Cu, Zn, or Al in brackish water (20‰, pH 8) for 96-hours. The exposures were conducted with nine concentrations of Cu and Zn, and six concentrations of Al. Several of the concentrations were the same, to directly compare the uptake between the metals. To investigate the uptake in fish; gills, kidneys, and livers were sampled at the end of the exposure. Metal concentrations were quantified using Inductively Coupled Plasma Mass spectrometry (ICP-MS). Water samples of the exposure water were size- and charge- fractionated in-situ at 0h and 96h, and analyzed later using ICP-MS. The results of size fractionation revealed that speciation of the Cu, Zn, and Al was relatively similar in brackish water, and was assumed to be bioavailable. Aluminum was found associated with the low molecular mass (LMM, <10kDa) fraction at >83% of the total, LMM-associated Zn at >78% of the total, while Cu had large uncertainties in this fraction. Copper was either associated with the colloidal or LMM fraction. Only a small percentage, <14%, of the metals were associated with particles. This low association is likely due to low organic content in the exposure water. The uptake of metals on fish followed the order, Al>Cu>Zn, where gills were the tissue with the highest uptake, kidneys intermediate and the liver had no uptake within ... |
author2 |
Teien, Hans-Christian Skipperud, Lindis Jaroz, Emil |
format |
Master Thesis |
author |
Nymo, Malene Therese |
author_facet |
Nymo, Malene Therese |
author_sort |
Nymo, Malene Therese |
title |
Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
title_short |
Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
title_full |
Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
title_fullStr |
Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
title_full_unstemmed |
Trace metal speciation and uptake in Atlantic salmon (Salmo salar) in coastal water |
title_sort |
trace metal speciation and uptake in atlantic salmon (salmo salar) in coastal water |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2724830 |
op_coverage |
Norway |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
96 |
op_relation |
Norwegian Research Council NRC: 268294 https://hdl.handle.net/11250/2724830 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
_version_ |
1771544129168211968 |