Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection
Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV...
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ftunivmob:oai:nmbu.brage.unit.no:11250/2690076 2023-05-15T15:30:29+02:00 Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection Kannimuthu, Dhamotharan Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria K. Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen 2020-07-23T08:55:32Z application/pdf https://hdl.handle.net/11250/2690076 https://doi.org/10.3390/pathogens9020143 eng eng Fiskeri- og havbruksnæringens forskningsfinansiering: 901305 Norges forskningsråd: 280847 Pathogens. 2020, 9 (2). urn:issn:2076-0817 https://hdl.handle.net/11250/2690076 https://doi.org/10.3390/pathogens9020143 cristin:1820247 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 9 Pathogens 2 143 Peer reviewed Journal article 2020 ftunivmob https://doi.org/10.3390/pathogens9020143 2021-09-23T20:16:55Z Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Histopathologic evaluation demonstrated that PRV-1 infection induced heart lesions typical of HSMI, such as severe epicarditis and myocarditis with degeneration of cardiomyocytes, necrosis, and diffuse cellular infiltration. PRV-1 infection of erythrocytes and the peak viral plasma level preceded virus presence in cardiomyocytes and hepatocytes. Arginase-2-positive, macrophage-like cells observed in the heart indicated possible polarization to M2 macrophages and the onset of regenerative processes, which may contribute to the recovery from HSMI. The virus was cleared from regenerating heart tissue and from hepatocytes, but persisted in erythrocytes. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Pathogens 9 2 143 |
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Open archive Norwegian University of Life Sciences: Brage NMBU |
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ftunivmob |
language |
English |
description |
Piscine orthoreovirus-1 (PRV-1) can cause heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar), but the line of events from infection, pathologic change, and regeneration has not been thoroughly described. In this study, the cellular localization and variation of PRV-1 RNA and protein levels were analyzed at different times post-exposure in experimentally infected Atlantic salmon. Immunohistochemistry, flow cytometry, and Western blot were used for assessment of the presence of the PRV-1 σ1 protein, while RT-qPCR and in situ hybridization were performed for viral RNA. Histopathologic evaluation demonstrated that PRV-1 infection induced heart lesions typical of HSMI, such as severe epicarditis and myocarditis with degeneration of cardiomyocytes, necrosis, and diffuse cellular infiltration. PRV-1 infection of erythrocytes and the peak viral plasma level preceded virus presence in cardiomyocytes and hepatocytes. Arginase-2-positive, macrophage-like cells observed in the heart indicated possible polarization to M2 macrophages and the onset of regenerative processes, which may contribute to the recovery from HSMI. The virus was cleared from regenerating heart tissue and from hepatocytes, but persisted in erythrocytes. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Kannimuthu, Dhamotharan Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria K. Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen |
spellingShingle |
Kannimuthu, Dhamotharan Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria K. Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
author_facet |
Kannimuthu, Dhamotharan Bjørgen, Håvard Malik, Muhammad Salman Nyman, Ingvild Berg Markussen, Turhan Ali Dadasbilge Dahle, Maria K. Koppang, Erling Olaf Wessel, Øystein Rimstad, Espen |
author_sort |
Kannimuthu, Dhamotharan |
title |
Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
title_short |
Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
title_full |
Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
title_fullStr |
Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
title_full_unstemmed |
Dissemination of piscine orthoreovirus-1 (PRV-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
title_sort |
dissemination of piscine orthoreovirus-1 (prv-1) in atlantic salmon (salmo salar) during the early and regenerating phases of infection |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2690076 https://doi.org/10.3390/pathogens9020143 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
9 Pathogens 2 143 |
op_relation |
Fiskeri- og havbruksnæringens forskningsfinansiering: 901305 Norges forskningsråd: 280847 Pathogens. 2020, 9 (2). urn:issn:2076-0817 https://hdl.handle.net/11250/2690076 https://doi.org/10.3390/pathogens9020143 cristin:1820247 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3390/pathogens9020143 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
2 |
container_start_page |
143 |
_version_ |
1766360925437165568 |