Inactivation of Piscine orthoreovirus
Piscine orthoreovirus infects various salmonid fish species, and the infection is associated with diseases such as heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). There are no vaccines available or genetically selected resistant hosts that can efficiently contr...
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Online Access: | https://hdl.handle.net/11250/2687958 https://doi.org/10.1111/jfd.13214 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/2687958 2023-05-15T15:32:19+02:00 Inactivation of Piscine orthoreovirus Wessel, Øystein Hansen, Elisabeth Furuseth Løvoll, Marie Inami, Makoto Husby, Asbjørn Kruse, Gesa Dahle, Maria Rimstad, Espen 2020-08-21T20:58:39Z application/pdf https://hdl.handle.net/11250/2687958 https://doi.org/10.1111/jfd.13214 eng eng Fiskeri- og havbruksnæringens forskningsfinansiering: 901305 urn:issn:0140-7775 https://hdl.handle.net/11250/2687958 https://doi.org/10.1111/jfd.13214 cristin:1824580 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 43 Journal of Fish Diseases 9 Peer reviewed Journal article 2020 ftunivmob https://doi.org/10.1111/jfd.13214 2021-09-23T20:15:04Z Piscine orthoreovirus infects various salmonid fish species, and the infection is associated with diseases such as heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). There are no vaccines available or genetically selected resistant hosts that can efficiently control piscine orthoreovirus (PRV) infection. Currently, the only prophylactic measure against PRV is general biosecurity measures aiming to break the transmission cycle. Methods to eradicate infectious virus from contaminated facilities are desirable, but the knowledge on how to inactivate PRV is lacking. A major bottleneck for inactivation studies is the lack of ability to propagate PRV in cell culture. Therefore, in this study we developed an in vivo model for detection of infectious PRV particles after treatment of the virus with inactivation tools such as heat, pH, iodine, UV and commercially available disinfectants. The results show that standard iodine treatment is efficient in inactivation of the virus, and similarly are high and low pH extremes and treatment with Virocid, a commercially available disinfectant. A UV dose of at least 50 mJ/cm2 is required for inactivation, and the virus has high resistance against heat treatment. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Journal of Fish Diseases 43 9 1039 1048 |
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Open archive Norwegian University of Life Sciences: Brage NMBU |
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ftunivmob |
language |
English |
description |
Piscine orthoreovirus infects various salmonid fish species, and the infection is associated with diseases such as heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). There are no vaccines available or genetically selected resistant hosts that can efficiently control piscine orthoreovirus (PRV) infection. Currently, the only prophylactic measure against PRV is general biosecurity measures aiming to break the transmission cycle. Methods to eradicate infectious virus from contaminated facilities are desirable, but the knowledge on how to inactivate PRV is lacking. A major bottleneck for inactivation studies is the lack of ability to propagate PRV in cell culture. Therefore, in this study we developed an in vivo model for detection of infectious PRV particles after treatment of the virus with inactivation tools such as heat, pH, iodine, UV and commercially available disinfectants. The results show that standard iodine treatment is efficient in inactivation of the virus, and similarly are high and low pH extremes and treatment with Virocid, a commercially available disinfectant. A UV dose of at least 50 mJ/cm2 is required for inactivation, and the virus has high resistance against heat treatment. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Wessel, Øystein Hansen, Elisabeth Furuseth Løvoll, Marie Inami, Makoto Husby, Asbjørn Kruse, Gesa Dahle, Maria Rimstad, Espen |
spellingShingle |
Wessel, Øystein Hansen, Elisabeth Furuseth Løvoll, Marie Inami, Makoto Husby, Asbjørn Kruse, Gesa Dahle, Maria Rimstad, Espen Inactivation of Piscine orthoreovirus |
author_facet |
Wessel, Øystein Hansen, Elisabeth Furuseth Løvoll, Marie Inami, Makoto Husby, Asbjørn Kruse, Gesa Dahle, Maria Rimstad, Espen |
author_sort |
Wessel, Øystein |
title |
Inactivation of Piscine orthoreovirus |
title_short |
Inactivation of Piscine orthoreovirus |
title_full |
Inactivation of Piscine orthoreovirus |
title_fullStr |
Inactivation of Piscine orthoreovirus |
title_full_unstemmed |
Inactivation of Piscine orthoreovirus |
title_sort |
inactivation of piscine orthoreovirus |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2687958 https://doi.org/10.1111/jfd.13214 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
43 Journal of Fish Diseases 9 |
op_relation |
Fiskeri- og havbruksnæringens forskningsfinansiering: 901305 urn:issn:0140-7775 https://hdl.handle.net/11250/2687958 https://doi.org/10.1111/jfd.13214 cristin:1824580 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1111/jfd.13214 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
9 |
container_start_page |
1039 |
op_container_end_page |
1048 |
_version_ |
1766362831562735616 |