Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the ful...
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Online Access: | https://hdl.handle.net/11250/2687431 https://doi.org/10.3389/fmicb.2020.00249 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/2687431 2023-05-15T16:57:03+02:00 Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy Stokke, Runar Reeves, Eoghan Dahle, Håkon Fedøy, Anita-Elin Viflot, Thomas Øfstegaard Onstad, Solveig L. Vulcano, Francesca Pedersen, Rolf Birger Eijsink, Vincent Steen, Ida Helene 2020-02-25T16:22:49Z application/pdf https://hdl.handle.net/11250/2687431 https://doi.org/10.3389/fmicb.2020.00249 eng eng Frontiers in Microbiology, 2020, 11, 249 urn:issn:1664-302X https://hdl.handle.net/11250/2687431 https://doi.org/10.3389/fmicb.2020.00249 cristin:1797447 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 11 Frontiers in Microbiology 249 Peer reviewed Journal article 2020 ftunivmob https://doi.org/10.3389/fmicb.2020.00249 2021-09-23T20:16:49Z Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme discovery for diverse industries. publishedVersion Article in Journal/Newspaper Jan Mayen Open archive Norwegian University of Life Sciences: Brage NMBU Jan Mayen Frontiers in Microbiology 11 |
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Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
description |
Deep-sea hydrothermal vents are amongst the most extreme environments on Earth and represent interesting targets for marine bioprospecting and biodiscovery. The microbial communities in hydrothermal vents are often dominated by chemolithoautotrophs utilizing simple chemical compounds, though the full extent of their heterotrophic abilities is still being explored. In the bioprocessing industry, where degradation of complex organic materials often is a major challenge, new microbial solutions are heavily needed. To meet these needs, we have developed novel in situ incubators and tested if deployment of recalcitrant materials from fish farming and wood-pulping industries introduced changes in the microbial community structure in hot marine hydrothermal sediments. The incubation chambers were deployed in sediments at the Bruse vent site located within the Jan Mayen vent field for 1 year, after which the microbial populations in the chambers were profiled by 16S rRNA Ion Torrent amplicon sequencing. A total of 921 operational taxonomic units (OTUs) were assigned into 74 different phyla where differences in community structure were observed depending on the incubated material, chamber depth below the sea floor and/or temperature. A high fraction of putative heterotrophic microbial lineages related to cultivated members within the Thermotogales were observed. However, considerable fractions of previously uncultivated and novel Thermotogales and Bacteroidetes were also identified. Moreover, several novel lineages (e.g., members within the DPANN superphylum, unidentified archaeal lineages, unclassified Thermoplasmatales and Candidatus division BRC-1 bacterium) of as-yet uncultivated thermophilic archaea and bacteria were identified. Overall, our data illustrate that amendment of hydrothermal vent communities by in situ incubation of biomass induces shifts in community structure toward increased fractions of heterotrophic microorganisms. The technologies utilized here could aid in subsequent metagenomics-based enzyme discovery for diverse industries. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Stokke, Runar Reeves, Eoghan Dahle, Håkon Fedøy, Anita-Elin Viflot, Thomas Øfstegaard Onstad, Solveig L. Vulcano, Francesca Pedersen, Rolf Birger Eijsink, Vincent Steen, Ida Helene |
spellingShingle |
Stokke, Runar Reeves, Eoghan Dahle, Håkon Fedøy, Anita-Elin Viflot, Thomas Øfstegaard Onstad, Solveig L. Vulcano, Francesca Pedersen, Rolf Birger Eijsink, Vincent Steen, Ida Helene Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
author_facet |
Stokke, Runar Reeves, Eoghan Dahle, Håkon Fedøy, Anita-Elin Viflot, Thomas Øfstegaard Onstad, Solveig L. Vulcano, Francesca Pedersen, Rolf Birger Eijsink, Vincent Steen, Ida Helene |
author_sort |
Stokke, Runar |
title |
Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
title_short |
Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
title_full |
Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
title_fullStr |
Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
title_full_unstemmed |
Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
title_sort |
tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2687431 https://doi.org/10.3389/fmicb.2020.00249 |
geographic |
Jan Mayen |
geographic_facet |
Jan Mayen |
genre |
Jan Mayen |
genre_facet |
Jan Mayen |
op_source |
11 Frontiers in Microbiology 249 |
op_relation |
Frontiers in Microbiology, 2020, 11, 249 urn:issn:1664-302X https://hdl.handle.net/11250/2687431 https://doi.org/10.3389/fmicb.2020.00249 cristin:1797447 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3389/fmicb.2020.00249 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
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1766048319783567360 |