Production, characterization, and application of an alginate lyase, AMOR_PL7A, from hot vents in the Arctic mid-ocean ridge

• Published in: Journal of Agricultural and Food Chemistry
• Main Authors: Vuoristo, Kiira, Fredriksen, Lasse, Oftebro, Maren, Arntzen, Magnus Øverlie, Aarstad, Olav Andreas, Stokke, Runar, Steen, Ida Helene, Hansen, Line Degn, Schüller, Reidar Barfod, Aachmann, Finn Lillelund, Horn, Svein Jarle, Eijsink, Vincent
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2019
• Subjects: Arctic
• Online Access: https://hdl.handle.net/11250/2660830; https://doi.org/10.1021/acs.jafc.8b07190

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-D-mannuronate specific (EC 4.2.2.3) endo acting alginate lyase, which degrades alginate and poly-mannuronate within a broad range of pH, temperature and salinity. At 65 oC and pH 6.0, its Km and kcat values for sodium alginate are 0.51 +/- 0.09 mg/mL and 7.8 +/- 0.3 s -1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 o C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing. acceptedVersion