Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis
Smoltification is a metamorphic event in salmon life history, which initiates downstream migration and pre-adapts juvenile salmon for seawater entry. While a number of reports concern thyroid hormones and smoltification, few and inconclusive studies have addressed the potential role of thyrotropin (...
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ftunivmob:oai:nmbu.brage.unit.no:11250/2603096 2023-05-15T15:30:36+02:00 Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis Fleming, Mitchell S. Maugars, Gersende Marie Aimee Lafont, Anne-Gaëlle Rancon, Jocelyn Fontaine, Romain Nourizadeh-Lillabadi, Rasoul Weltzien, Finn-Arne Yebra-Pimente, Elena Santidrian Dirks, Ron McCormick, Stephen D. Rousseau, Karine Martin, Patrick Dufour, Sylvie 2019-03-17T18:03:51Z application/pdf http://hdl.handle.net/11250/2603096 https://doi.org/10.1038/s41598-019-40019-5 eng eng urn:issn:2045-2322 http://hdl.handle.net/11250/2603096 https://doi.org/10.1038/s41598-019-40019-5 cristin:1685397 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 9 Scientific Reports Journal article Peer reviewed 2019 ftunivmob https://doi.org/10.1038/s41598-019-40019-5 2021-09-23T20:15:25Z Smoltification is a metamorphic event in salmon life history, which initiates downstream migration and pre-adapts juvenile salmon for seawater entry. While a number of reports concern thyroid hormones and smoltification, few and inconclusive studies have addressed the potential role of thyrotropin (TSH). TSH is composed of a α-subunit common to gonadotropins, and a β-subunit conferring hormone specificity. We report the presence and functional divergence of duplicated TSH β-subunit paralogs (tshβa and tshβb) in Atlantic salmon. Phylogeny and synteny analyses allowed us to infer that they originated from teleost-specific whole genome duplication. Expression profiles of both paralogs in the pituitary were measured by qPCR throughout smoltification in Atlantic salmon from the endangered Loire-Allier population raised in a conservation hatchery. This revealed a striking peak of tshβb expression in April, concomitant with downstream migration initiation, while tshβa expression remained relatively constant. In situ hybridization showed two distinct pituitary cell populations, tshβa cells in the anterior adenohypophysis, and tshβb cells near to the pituitary stalk, a location comparable to the pars tuberalis TSH cells involved in seasonal physiology and behaviour in birds and mammals. Functional divergence of tshβ paralogs in Atlantic salmon supports a specific role of tshβb in smoltification. publishedVersion Article in Journal/Newspaper Atlantic salmon Open archive Norwegian University of Life Sciences: Brage NMBU Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
description |
Smoltification is a metamorphic event in salmon life history, which initiates downstream migration and pre-adapts juvenile salmon for seawater entry. While a number of reports concern thyroid hormones and smoltification, few and inconclusive studies have addressed the potential role of thyrotropin (TSH). TSH is composed of a α-subunit common to gonadotropins, and a β-subunit conferring hormone specificity. We report the presence and functional divergence of duplicated TSH β-subunit paralogs (tshβa and tshβb) in Atlantic salmon. Phylogeny and synteny analyses allowed us to infer that they originated from teleost-specific whole genome duplication. Expression profiles of both paralogs in the pituitary were measured by qPCR throughout smoltification in Atlantic salmon from the endangered Loire-Allier population raised in a conservation hatchery. This revealed a striking peak of tshβb expression in April, concomitant with downstream migration initiation, while tshβa expression remained relatively constant. In situ hybridization showed two distinct pituitary cell populations, tshβa cells in the anterior adenohypophysis, and tshβb cells near to the pituitary stalk, a location comparable to the pars tuberalis TSH cells involved in seasonal physiology and behaviour in birds and mammals. Functional divergence of tshβ paralogs in Atlantic salmon supports a specific role of tshβb in smoltification. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Fleming, Mitchell S. Maugars, Gersende Marie Aimee Lafont, Anne-Gaëlle Rancon, Jocelyn Fontaine, Romain Nourizadeh-Lillabadi, Rasoul Weltzien, Finn-Arne Yebra-Pimente, Elena Santidrian Dirks, Ron McCormick, Stephen D. Rousseau, Karine Martin, Patrick Dufour, Sylvie |
spellingShingle |
Fleming, Mitchell S. Maugars, Gersende Marie Aimee Lafont, Anne-Gaëlle Rancon, Jocelyn Fontaine, Romain Nourizadeh-Lillabadi, Rasoul Weltzien, Finn-Arne Yebra-Pimente, Elena Santidrian Dirks, Ron McCormick, Stephen D. Rousseau, Karine Martin, Patrick Dufour, Sylvie Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
author_facet |
Fleming, Mitchell S. Maugars, Gersende Marie Aimee Lafont, Anne-Gaëlle Rancon, Jocelyn Fontaine, Romain Nourizadeh-Lillabadi, Rasoul Weltzien, Finn-Arne Yebra-Pimente, Elena Santidrian Dirks, Ron McCormick, Stephen D. Rousseau, Karine Martin, Patrick Dufour, Sylvie |
author_sort |
Fleming, Mitchell S. |
title |
Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
title_short |
Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
title_full |
Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
title_fullStr |
Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
title_full_unstemmed |
Functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
title_sort |
functional divergence of thyrotropin beta-subunit paralogs gives new insights into salmon smoltification metamorphosis |
publishDate |
2019 |
url |
http://hdl.handle.net/11250/2603096 https://doi.org/10.1038/s41598-019-40019-5 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
9 Scientific Reports |
op_relation |
urn:issn:2045-2322 http://hdl.handle.net/11250/2603096 https://doi.org/10.1038/s41598-019-40019-5 cristin:1685397 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1038/s41598-019-40019-5 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766361049525649408 |