Characterization of a thermostable exolytic alginate lyase from hot vents in the Arctic Mid-Ocean Ridge

• Main Author: Pedersen, Bjørn
• Other Authors: Eijsink, Vincent, Fredriksen, Lasse, Arntzen, Magnus Øverlie
• Format: Master Thesis
• Language: English
• Published: Norwegian University of Life Sciences, Ås 2019
• Subjects: Enzyme characterization; DNS assay; HPAEC; VDP::Teknologi: 500::Bioteknologi: 590; Arctic; Norway; Brune
• Online Access: http://hdl.handle.net/11250/2602511

Verdens behov for petroleumsprodukter øker stadig, men minkende oljereserver og miljøproblemer gjør at det bør prioriteres utvikling og bruk av alternative, fornybare ressurser. Bioraffinering dreier seg om å bruke biomasse som karbonkilde for produksjon av energi og nyttige kjemikalier/materialer. Makroalger er et eksempel på en biomasse med egenskaper som gjør den relevant for bruk i framtidige bioraffinerier. Denne studien undersøker egenskapene til et rekombinant enzym, Apl17-2, som depolymeriserer alginat, som er en viktig bestanddel av brune makroalger. The world’s demand for petrochemicals is rising, but depletion of oil reserves and environmental concerns encourage the utilisation of alternative, renewable resources. Biorefineries are based around the concept of using biomass as a carbon source for generating energy and useful chemicals/materials. Macroalgae (seaweed) have properties that make them and interesting and relevant biomass for use in future biorefineries. This study investigates the properties of a recombinantly produced enzyme, Apl17-2, that depolymerises alginate, which is a major component of brown macroalgae. Foods of Norway submittedVersion M-BIOTEK