Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss)
Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in diferent salmonid species. PRV-1causes heart and skeletal muscle infammation (HSMI) in Atlantic salmon (Salm...
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ftunivmob:oai:nmbu.brage.unit.no:11250/2602052 2023-05-15T15:31:39+02:00 Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Rimstad, Espen Olesen, Niels Jørgen 2019-02-13T16:33:25Z application/pdf http://hdl.handle.net/11250/2602052 https://doi.org/10.1186/s13567-019-0632-4 eng eng urn:issn:0928-4249 http://hdl.handle.net/11250/2602052 https://doi.org/10.1186/s13567-019-0632-4 cristin:1677174 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no Veterinary research (Print) Journal article Peer reviewed 2019 ftunivmob https://doi.org/10.1186/s13567-019-0632-4 2023-03-22T23:45:32Z Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in diferent salmonid species. PRV-1causes heart and skeletal muscle infammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purifed PRV-3 particles to assess the causal relationship between the virus and development of heart infammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological fndings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The fndings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Veterinary Research 50 1 |
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Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
description |
Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in diferent salmonid species. PRV-1causes heart and skeletal muscle infammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purifed PRV-3 particles to assess the causal relationship between the virus and development of heart infammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological fndings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The fndings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Rimstad, Espen Olesen, Niels Jørgen |
spellingShingle |
Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Rimstad, Espen Olesen, Niels Jørgen Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
author_facet |
Vendramin, Niccoló Kannimuthu, Dhamotharan Olsen, Anne Berit Cuenca, Argelia Teige, Lena Hammerlund Wessel, Øystein Iburg, Tine Moesgaard Dahle, Maria Rimstad, Espen Olesen, Niels Jørgen |
author_sort |
Vendramin, Niccoló |
title |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_short |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_sort |
piscine orthoreovirus subtype 3 (prv-3) causes heart inflammation in rainbow trout (oncorhynchus mykiss) |
publishDate |
2019 |
url |
http://hdl.handle.net/11250/2602052 https://doi.org/10.1186/s13567-019-0632-4 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Veterinary research (Print) |
op_relation |
urn:issn:0928-4249 http://hdl.handle.net/11250/2602052 https://doi.org/10.1186/s13567-019-0632-4 cristin:1677174 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_doi |
https://doi.org/10.1186/s13567-019-0632-4 |
container_title |
Veterinary Research |
container_volume |
50 |
container_issue |
1 |
_version_ |
1766362180592074752 |