Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss)
Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout (Oncorhynchus mykiss) with HSMI-like disease. PRV is considered to be an emerging pathoge...
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ftunivmob:oai:nmbu.brage.unit.no:11250/2569552 2023-05-15T15:32:42+02:00 Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) Kannimuthu, Dhamotharan Vendramin, Niccolò Markussen, Turhan Wessel, Øystein Cuenca, Argelia Nyman, Ingvild Berg Olsen, Anne Berit Tengs, Torstein Dahle, Maria Rimstad, Espen 2018-05-12T13:34:09Z application/pdf http://hdl.handle.net/11250/2569552 https://doi.org/10.3390/v10040170 eng eng http://www.mdpi.com/1999-4915/10/4/170/htm Norges forskningsråd: 237315/E40 Viruses. 2018, 10 (4), 1-16. urn:issn:1999-4915 http://hdl.handle.net/11250/2569552 https://doi.org/10.3390/v10040170 cristin:1584680 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND 1-16 10 Viruses 4 Journal article Peer reviewed 2018 ftunivmob https://doi.org/10.3390/v10040170 2021-09-23T20:15:27Z Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout (Oncorhynchus mykiss) with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus. Virus particles were purified by gradient ultracentrifugation and the complete coding sequences of PRV-3 were obtained by Illumina sequencing. When compared to PRV-1, the nucleotide identity of the coding regions was 80.1%, and the amino acid identities of the predicted PRV-3 proteins varied from 96.7% (λ1) to 79.1% (σ3). Phylogenetic analysis showed that PRV-3 belongs to a separate cluster. The region encoding σ3 were sequenced from PRV-3 isolates collected from rainbow trout in Europe. These sequences clustered together, but were distant from PRV-3 that was isolated from rainbow trout in Norway. Bioinformatic analyses of PRV-3 proteins revealed that predicted secondary structures and functional domains were conserved between PRV-3 and PRV-1. Rabbit antisera raised against purified virus or various recombinant virus proteins from PRV-1 all cross-reacted with PRV-3. Our findings indicate that despite different species preferences of the PRV subtypes, several genetic, antigenic, and structural properties are conserved between PRV-1 and-3. Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Norway Viruses 10 4 170 |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
description |
Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout (Oncorhynchus mykiss) with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus. Virus particles were purified by gradient ultracentrifugation and the complete coding sequences of PRV-3 were obtained by Illumina sequencing. When compared to PRV-1, the nucleotide identity of the coding regions was 80.1%, and the amino acid identities of the predicted PRV-3 proteins varied from 96.7% (λ1) to 79.1% (σ3). Phylogenetic analysis showed that PRV-3 belongs to a separate cluster. The region encoding σ3 were sequenced from PRV-3 isolates collected from rainbow trout in Europe. These sequences clustered together, but were distant from PRV-3 that was isolated from rainbow trout in Norway. Bioinformatic analyses of PRV-3 proteins revealed that predicted secondary structures and functional domains were conserved between PRV-3 and PRV-1. Rabbit antisera raised against purified virus or various recombinant virus proteins from PRV-1 all cross-reacted with PRV-3. Our findings indicate that despite different species preferences of the PRV subtypes, several genetic, antigenic, and structural properties are conserved between PRV-1 and-3. Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) publishedVersion |
format |
Article in Journal/Newspaper |
author |
Kannimuthu, Dhamotharan Vendramin, Niccolò Markussen, Turhan Wessel, Øystein Cuenca, Argelia Nyman, Ingvild Berg Olsen, Anne Berit Tengs, Torstein Dahle, Maria Rimstad, Espen |
spellingShingle |
Kannimuthu, Dhamotharan Vendramin, Niccolò Markussen, Turhan Wessel, Øystein Cuenca, Argelia Nyman, Ingvild Berg Olsen, Anne Berit Tengs, Torstein Dahle, Maria Rimstad, Espen Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
author_facet |
Kannimuthu, Dhamotharan Vendramin, Niccolò Markussen, Turhan Wessel, Øystein Cuenca, Argelia Nyman, Ingvild Berg Olsen, Anne Berit Tengs, Torstein Dahle, Maria Rimstad, Espen |
author_sort |
Kannimuthu, Dhamotharan |
title |
Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
title_short |
Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
title_full |
Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
title_fullStr |
Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
title_full_unstemmed |
Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss) |
title_sort |
molecular and antigenic characterization of piscine orthoreovirus (prv) from rainbow trout (oncorhynchus orthoreovirumykiss) |
publishDate |
2018 |
url |
http://hdl.handle.net/11250/2569552 https://doi.org/10.3390/v10040170 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
1-16 10 Viruses 4 |
op_relation |
http://www.mdpi.com/1999-4915/10/4/170/htm Norges forskningsråd: 237315/E40 Viruses. 2018, 10 (4), 1-16. urn:issn:1999-4915 http://hdl.handle.net/11250/2569552 https://doi.org/10.3390/v10040170 cristin:1584680 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.3390/v10040170 |
container_title |
Viruses |
container_volume |
10 |
container_issue |
4 |
container_start_page |
170 |
_version_ |
1766363188428800000 |