Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes
Viral diseases are vital challenges in the aquaculture industry. In Norway, heart- and skeletal muscle inflammation (HSMI) is one of the major viral diseases affecting Atlantic salmon. In mammals, low vitamin D has been found to increase susceptibility to viral diseases. In this study, the overall a...
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Norwegian University of Life Sciences, Ås
2017
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Online Access: | http://hdl.handle.net/11250/2492603 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/2492603 2023-05-15T15:30:28+02:00 Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes Mohammed, Abdelaziz Adam Abdelkarim Rimstad, Espen 2017 application/pdf http://hdl.handle.net/11250/2492603 eng eng Norwegian University of Life Sciences, Ås http://hdl.handle.net/11250/2492603 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND Master thesis 2017 ftunivmob 2021-09-23T20:14:45Z Viral diseases are vital challenges in the aquaculture industry. In Norway, heart- and skeletal muscle inflammation (HSMI) is one of the major viral diseases affecting Atlantic salmon. In mammals, low vitamin D has been found to increase susceptibility to viral diseases. In this study, the overall aim was to determine if vitamin D2 and D3 together with the RXR agonist bexarotene have an effect on infection and antiviral response against Piscine orthroreovirus (PRV), the dsRNA virus that causes HSMI. The main target cells for PRV in vivo are the erythrocytes (red blood cells), which can also be infected ex vivo. To achieve the study objectives, three experiments were performed. Naïve or infected RBCs were isolated from Atlantic salmon, incubated with vitamin D, and naïve cells were either poly I: C stimulated or treated with purified PRV. The virus replication and antiviral gene expression were analyzed by RT-qPCR. The results indicated that vitamin D may potentiate the antiviral response, although results were inconclusive. The study indicates that vitamin D may have an effect on antiviral responses, but this needs to be further investigated. submittedVersion M-FT Master Thesis Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Norway |
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Open Polar |
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Open archive Norwegian University of Life Sciences: Brage NMBU |
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ftunivmob |
language |
English |
description |
Viral diseases are vital challenges in the aquaculture industry. In Norway, heart- and skeletal muscle inflammation (HSMI) is one of the major viral diseases affecting Atlantic salmon. In mammals, low vitamin D has been found to increase susceptibility to viral diseases. In this study, the overall aim was to determine if vitamin D2 and D3 together with the RXR agonist bexarotene have an effect on infection and antiviral response against Piscine orthroreovirus (PRV), the dsRNA virus that causes HSMI. The main target cells for PRV in vivo are the erythrocytes (red blood cells), which can also be infected ex vivo. To achieve the study objectives, three experiments were performed. Naïve or infected RBCs were isolated from Atlantic salmon, incubated with vitamin D, and naïve cells were either poly I: C stimulated or treated with purified PRV. The virus replication and antiviral gene expression were analyzed by RT-qPCR. The results indicated that vitamin D may potentiate the antiviral response, although results were inconclusive. The study indicates that vitamin D may have an effect on antiviral responses, but this needs to be further investigated. submittedVersion M-FT |
author2 |
Rimstad, Espen |
format |
Master Thesis |
author |
Mohammed, Abdelaziz Adam Abdelkarim |
spellingShingle |
Mohammed, Abdelaziz Adam Abdelkarim Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
author_facet |
Mohammed, Abdelaziz Adam Abdelkarim |
author_sort |
Mohammed, Abdelaziz Adam Abdelkarim |
title |
Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
title_short |
Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
title_full |
Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
title_fullStr |
Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
title_full_unstemmed |
Effects of fat soluble vitamins on viral infection and antiviral responses in Atlantic salmon (Salmo salar L.) erythrocytes |
title_sort |
effects of fat soluble vitamins on viral infection and antiviral responses in atlantic salmon (salmo salar l.) erythrocytes |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2017 |
url |
http://hdl.handle.net/11250/2492603 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://hdl.handle.net/11250/2492603 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766360914767904768 |