Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type
A better understanding of how emulsifier type could differently influence the behavior of nanostructured lipid carriers (NLC) under the gastrointestinal digestion process, as well as at the cellular level, is of utmost importance for the NLC-based formulations optimization and risk assessment in the...
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Online Access: | https://hdl.handle.net/1822/91946 https://doi.org/10.1111/1750-3841.17113 |
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ftunivminho:oai:repositorium.sdum.uminho.pt:1822/91946 2024-09-15T18:14:42+00:00 Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type Lüdtke, Fernanda Luisa Fernandes, Jean Michel Gonçalves, Raquel Filipa Silva Martins, Joana T. Berni, Paulo Ribeiro, Ana P. B. Vicente, A. A. Pinheiro, A. C. 2024-06 application/pdf https://hdl.handle.net/1822/91946 https://doi.org/10.1111/1750-3841.17113 eng eng Wiley-Blackwell info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147286%2F2019/PT info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F140182%2F2018/PT 2022.00788.CEECIND/CP1718/CT0024 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT LA/P/0029/2020 https://ift.onlinelibrary.wiley.com/journal/17503841 Lüdtke, Fernanda Luisa; Fernandes, J. -M.; Gonçalves, Raquel F. S.; Martins, Joana T.; Berni, P.; Ribeiro, Ana P. B.; Vicente, António A.; Pinheiro, Ana Cristina, Performance of -carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: Influence of the emulsifier type. Journal of Food Science, 89(6), 3290-3305, 2024 0022-1147 https://hdl.handle.net/1822/91946 1750-3841 doi:10.1111/1750-3841.17113 info:eu-repo/semantics/openAccess Carotenoids Cell viability Dynamic in vitro gastrointestinal system Lipid nanoparticles info:eu-repo/semantics/article 2024 ftunivminho https://doi.org/10.1111/1750-3841.17113 2024-06-26T00:12:28Z A better understanding of how emulsifier type could differently influence the behavior of nanostructured lipid carriers (NLC) under the gastrointestinal digestion process, as well as at the cellular level, is of utmost importance for the NLC-based formulations optimization and risk assessment in the food field. In this study, NLC composed by fully hydrogenated soybean and high-oleic sunflower oils were prepared using soy lecithin (NLC L) or Tween 80 (NLC T) as an emulsifier. -Carotene was entrapped within NLC developed as a promising strategy to overcome -carotene's low bioavailability and stability. The effect of emulsifier type on the digestibility of -carotene-loaded NLC was evaluated using an in vitro dynamic digestion model mimicking peristalsis motion. The influence of -carotene-loaded NLC on cell viability was assessed using Caco-2 cells in vitro. NLC T remained stable in the gastric compartment, presenting particle size (PS) similar to the initial NLC (PS: 245.68 and 218.18 nm, respectively), while NLC L showed lower stability (PS > 1000 nm) in stomach and duodenum phases. NLC T also provided high -carotene protection and delivery capacity (i.e., -carotene bioaccessibility increased 10-fold). Based on the results of digestion studies, NLC T has shown better physical stability during the passage through the in vitro dynamic gastrointestinal system than NLC L. Moreover, the developed NLC did not compromise cell viability up to 25 µg/mL of -carotene. Thus, the NLC developed proved to be a biocompatible structure and able to incorporate and protect -carotene for further food applications. F.L.L. and P.B. acknowledge the Sao Paulo Research Foundation (FAPESP) for the financial support (grants #2018/03172-0, #2019/05176-6, #2019/27354-3, and #2021/02271-8). F.L.L. also thanks Iceland Liechten-stein Norway Grants (PT-INNOVATION-0105) for the fellowship. J.-M.F. and R.G. acknowledge the Foundation for Science and Technology (FCT) for their fellowships (SFRH/BD/147286/2019 and SFRH/BD/140182/2018, ... Article in Journal/Newspaper Iceland Universidade of Minho: RepositóriUM Journal of Food Science 89 6 3290 3305 |
institution |
Open Polar |
collection |
Universidade of Minho: RepositóriUM |
op_collection_id |
ftunivminho |
language |
English |
topic |
Carotenoids Cell viability Dynamic in vitro gastrointestinal system Lipid nanoparticles |
spellingShingle |
Carotenoids Cell viability Dynamic in vitro gastrointestinal system Lipid nanoparticles Lüdtke, Fernanda Luisa Fernandes, Jean Michel Gonçalves, Raquel Filipa Silva Martins, Joana T. Berni, Paulo Ribeiro, Ana P. B. Vicente, A. A. Pinheiro, A. C. Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
topic_facet |
Carotenoids Cell viability Dynamic in vitro gastrointestinal system Lipid nanoparticles |
description |
A better understanding of how emulsifier type could differently influence the behavior of nanostructured lipid carriers (NLC) under the gastrointestinal digestion process, as well as at the cellular level, is of utmost importance for the NLC-based formulations optimization and risk assessment in the food field. In this study, NLC composed by fully hydrogenated soybean and high-oleic sunflower oils were prepared using soy lecithin (NLC L) or Tween 80 (NLC T) as an emulsifier. -Carotene was entrapped within NLC developed as a promising strategy to overcome -carotene's low bioavailability and stability. The effect of emulsifier type on the digestibility of -carotene-loaded NLC was evaluated using an in vitro dynamic digestion model mimicking peristalsis motion. The influence of -carotene-loaded NLC on cell viability was assessed using Caco-2 cells in vitro. NLC T remained stable in the gastric compartment, presenting particle size (PS) similar to the initial NLC (PS: 245.68 and 218.18 nm, respectively), while NLC L showed lower stability (PS > 1000 nm) in stomach and duodenum phases. NLC T also provided high -carotene protection and delivery capacity (i.e., -carotene bioaccessibility increased 10-fold). Based on the results of digestion studies, NLC T has shown better physical stability during the passage through the in vitro dynamic gastrointestinal system than NLC L. Moreover, the developed NLC did not compromise cell viability up to 25 µg/mL of -carotene. Thus, the NLC developed proved to be a biocompatible structure and able to incorporate and protect -carotene for further food applications. F.L.L. and P.B. acknowledge the Sao Paulo Research Foundation (FAPESP) for the financial support (grants #2018/03172-0, #2019/05176-6, #2019/27354-3, and #2021/02271-8). F.L.L. also thanks Iceland Liechten-stein Norway Grants (PT-INNOVATION-0105) for the fellowship. J.-M.F. and R.G. acknowledge the Foundation for Science and Technology (FCT) for their fellowships (SFRH/BD/147286/2019 and SFRH/BD/140182/2018, ... |
format |
Article in Journal/Newspaper |
author |
Lüdtke, Fernanda Luisa Fernandes, Jean Michel Gonçalves, Raquel Filipa Silva Martins, Joana T. Berni, Paulo Ribeiro, Ana P. B. Vicente, A. A. Pinheiro, A. C. |
author_facet |
Lüdtke, Fernanda Luisa Fernandes, Jean Michel Gonçalves, Raquel Filipa Silva Martins, Joana T. Berni, Paulo Ribeiro, Ana P. B. Vicente, A. A. Pinheiro, A. C. |
author_sort |
Lüdtke, Fernanda Luisa |
title |
Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
title_short |
Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
title_full |
Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
title_fullStr |
Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
title_full_unstemmed |
Performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
title_sort |
performance of β-carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: influence of the emulsifier type |
publisher |
Wiley-Blackwell |
publishDate |
2024 |
url |
https://hdl.handle.net/1822/91946 https://doi.org/10.1111/1750-3841.17113 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F147286%2F2019/PT info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F140182%2F2018/PT 2022.00788.CEECIND/CP1718/CT0024 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT LA/P/0029/2020 https://ift.onlinelibrary.wiley.com/journal/17503841 Lüdtke, Fernanda Luisa; Fernandes, J. -M.; Gonçalves, Raquel F. S.; Martins, Joana T.; Berni, P.; Ribeiro, Ana P. B.; Vicente, António A.; Pinheiro, Ana Cristina, Performance of -carotene-loaded nanostructured lipid carriers under dynamic in vitro digestion system: Influence of the emulsifier type. Journal of Food Science, 89(6), 3290-3305, 2024 0022-1147 https://hdl.handle.net/1822/91946 1750-3841 doi:10.1111/1750-3841.17113 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1750-3841.17113 |
container_title |
Journal of Food Science |
container_volume |
89 |
container_issue |
6 |
container_start_page |
3290 |
op_container_end_page |
3305 |
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1810452481365245952 |