Maximization of fructose esters synthesis by response surface methodology

• Published in: New Biotechnology
• Main Authors: Neta, Nair do Amaral Sampaio, Peres, A. M., Teixeira, J. A., Rodrigues, L. R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: European Federation of Biotechnology (EFB) 2011
• Subjects: Candida antartica B; Fructose esters; Response surface methodology; Science & Technology; antartic*
• Online Access: http://hdl.handle.net/1822/13063; https://doi.org/10.1016/j.nbt.2011.02.007

Enzymatic synthesis of fructose fatty acide sterwasper formed inorganic solventmedia, using apurified lipase from Candida antartica B immobilizedinacrylicresin. Response sur face methodology with a central composit erotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature andre action time were found to bethe most significant parameters. The optimum operational conditions for maximizing the synthesis of fructos e esters were 57.18C, 100rpm and 37.8h. The model was validated in the identified optimal condition stocheck its adequacy and accuracy, and an experimental esterification percentage of 88.4% ( 0.3%) was obtained. These results showed that animprovement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions The authors acknowledge the AlBan Programme (European Union Programme of High Level Scholarships for Latin America) for financial support of N. Lopes through the research grant E07D401544BR. Also, the authors acknowledge the Department of Chemistry from University of Minho (Portugal) for running the infrared spectroscopy ...