Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1
Ice-binding proteins (IBPs) have been identified in numerous polar algae and bacteria, but so far not in any cyanobacteria, despite the abundance of cyanobacteria in polar regions. We previously reported strong IBP activity associated with an Antarctic Nostoc species. In this study, to identify the...
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Online Access: | http://hdl.handle.net/10281/298488 https://doi.org/10.1128/aem.02499-20 |
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ftunivmilanobic:oai:boa.unimib.it:10281/298488 2024-04-14T08:01:41+00:00 Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 James A. Raymond Michael G. Janech Marco Mangiagalli Raymond, J Janech, M Mangiagalli, M 2021 http://hdl.handle.net/10281/298488 https://doi.org/10.1128/aem.02499-20 eng eng American Society for Microbiology country:US info:eu-repo/semantics/altIdentifier/pmid/33158891 info:eu-repo/semantics/altIdentifier/wos/WOS:000605459800025 volume:87 issue:2 firstpage:1 lastpage:12 numberofpages:12 journal:APPLIED AND ENVIRONMENTAL MICROBIOLOGY http://hdl.handle.net/10281/298488 doi:10.1128/aem.02499-20 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85099427610 info:eu-repo/semantics/openAccess Antarctica horizontal gene transfer icebinding protein Nostoc PEP C-terminal signal info:eu-repo/semantics/article 2021 ftunivmilanobic https://doi.org/10.1128/aem.02499-20 2024-03-21T17:17:43Z Ice-binding proteins (IBPs) have been identified in numerous polar algae and bacteria, but so far not in any cyanobacteria, despite the abundance of cyanobacteria in polar regions. We previously reported strong IBP activity associated with an Antarctic Nostoc species. In this study, to identify the proteins responsible, as well as elucidate their origin, we sequenced the DNA of an environmental sample of this species, designated Nostoc sp. HG1, and its bacterial community and attempted to identify IBPs by looking for known IBPs in the metagenome and by looking for novel IBPs by tandem mass spectrometry (MS/MS) proteomics analyses of ice affinity-purified proteins. The metagenome contained over 116 DUF3494-type IBP genes, the most common type of IBP identified so far. One of the IBPs could be confidently assigned to Nostoc, while the others could be attributed to diverse bacteria, which, surprisingly, accounted for the great majority of the metagenome. Recombinant Nostoc IBPs (nIBPs) had strong ice-structuring activities, and their circular dichroism spectra were consistent with the secondary structure of a DUF3494-type IBP. nIBP is unusual in that it is the only IBP identified so far to have a PEP (amino acid motif) C-terminal signal, a signal that has been associated with anchoring to the outer cell membrane. These results suggest that the observed IBP activity of Nostoc sp. HG1 was due to a combination of endogenous and exogenous IBPs. Amino acid and nucleotide sequence analyses of nIBP raise the possibility that it was acquired from a planctomycete Article in Journal/Newspaper Antarc* Antarctic Antarctica Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) Antarctic Applied and Environmental Microbiology 87 2 |
institution |
Open Polar |
collection |
Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) |
op_collection_id |
ftunivmilanobic |
language |
English |
topic |
Antarctica horizontal gene transfer icebinding protein Nostoc PEP C-terminal signal |
spellingShingle |
Antarctica horizontal gene transfer icebinding protein Nostoc PEP C-terminal signal James A. Raymond Michael G. Janech Marco Mangiagalli Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
topic_facet |
Antarctica horizontal gene transfer icebinding protein Nostoc PEP C-terminal signal |
description |
Ice-binding proteins (IBPs) have been identified in numerous polar algae and bacteria, but so far not in any cyanobacteria, despite the abundance of cyanobacteria in polar regions. We previously reported strong IBP activity associated with an Antarctic Nostoc species. In this study, to identify the proteins responsible, as well as elucidate their origin, we sequenced the DNA of an environmental sample of this species, designated Nostoc sp. HG1, and its bacterial community and attempted to identify IBPs by looking for known IBPs in the metagenome and by looking for novel IBPs by tandem mass spectrometry (MS/MS) proteomics analyses of ice affinity-purified proteins. The metagenome contained over 116 DUF3494-type IBP genes, the most common type of IBP identified so far. One of the IBPs could be confidently assigned to Nostoc, while the others could be attributed to diverse bacteria, which, surprisingly, accounted for the great majority of the metagenome. Recombinant Nostoc IBPs (nIBPs) had strong ice-structuring activities, and their circular dichroism spectra were consistent with the secondary structure of a DUF3494-type IBP. nIBP is unusual in that it is the only IBP identified so far to have a PEP (amino acid motif) C-terminal signal, a signal that has been associated with anchoring to the outer cell membrane. These results suggest that the observed IBP activity of Nostoc sp. HG1 was due to a combination of endogenous and exogenous IBPs. Amino acid and nucleotide sequence analyses of nIBP raise the possibility that it was acquired from a planctomycete |
author2 |
Raymond, J Janech, M Mangiagalli, M |
format |
Article in Journal/Newspaper |
author |
James A. Raymond Michael G. Janech Marco Mangiagalli |
author_facet |
James A. Raymond Michael G. Janech Marco Mangiagalli |
author_sort |
James A. Raymond |
title |
Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
title_short |
Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
title_full |
Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
title_fullStr |
Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
title_full_unstemmed |
Ice-binding proteins associated with an Antarctic cyanobacterium, Nostoc sp. HG1 |
title_sort |
ice-binding proteins associated with an antarctic cyanobacterium, nostoc sp. hg1 |
publisher |
American Society for Microbiology |
publishDate |
2021 |
url |
http://hdl.handle.net/10281/298488 https://doi.org/10.1128/aem.02499-20 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/33158891 info:eu-repo/semantics/altIdentifier/wos/WOS:000605459800025 volume:87 issue:2 firstpage:1 lastpage:12 numberofpages:12 journal:APPLIED AND ENVIRONMENTAL MICROBIOLOGY http://hdl.handle.net/10281/298488 doi:10.1128/aem.02499-20 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85099427610 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/aem.02499-20 |
container_title |
Applied and Environmental Microbiology |
container_volume |
87 |
container_issue |
2 |
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1796310313863217152 |