A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain
Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, redu...
Published in: | Frontiers in Microbiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2023
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Online Access: | https://hdl.handle.net/2434/954240 https://doi.org/10.3389/fmicb.2023.1078382 |
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ftunivmilanoair:oai:air.unimi.it:2434/954240 2024-02-04T09:55:11+01:00 A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain Bisaccia, Melissa Binda, Elisa Rosini, Elena Caruso, Gabriella Dell'Acqua, Ombretta Azzaro, Maurizio Laganà, Pasqualina Tedeschi, Gabriella Maffioli, Elisa M. Pollegioni, Loredano Marinelli, Flavia M. Bisaccia E. Binda E. Rosini G. Caruso O. Dell'Acqua M. Azzaro P. Laganà G. Tedeschi E.M. Maffioli L. Pollegioni F. Marinelli 2023-02-10 https://hdl.handle.net/2434/954240 https://doi.org/10.3389/fmicb.2023.1078382 eng eng Frontiers Research Foundation info:eu-repo/semantics/altIdentifier/wos/WOS:000937648000001 volume:14 firstpage:1 lastpage:13 numberofpages:13 journal:FRONTIERS IN MICROBIOLOGY https://hdl.handle.net/2434/954240 doi:10.3389/fmicb.2023.1078382 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85148762435 info:eu-repo/semantics/openAccess Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Settore BIO/10 - Biochimica info:eu-repo/semantics/article 2023 ftunivmilanoair https://doi.org/10.3389/fmicb.2023.1078382 2024-01-09T23:49:52Z Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, reduce contaminations, and minimize pretreatment steps. Here, we report on the screening of 186 morphologically diverse microorganisms isolated from marine biofilms and water samples collected in Terra Nova Bay (Ross Sea, Antarctica) for the identification of new laccase activities. After primary screening, 13.4 and 10.8% of the isolates were identified for the ability to oxidize 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the dye azure B, respectively. Amongst them, the marine Halomonas sp. strain M68 showed the highest activity. Production of its laccase-like activity increased six-fold when copper was added to culture medium. Enzymatic activity-guided separation coupled with mass spectrometry identified this intracellular laccase-like protein (named Ant laccase) as belonging to the copper resistance system multicopper oxidase family. Ant laccase oxidized ABTS and 2,6-dimethoxy phenol, working better at acidic pHs The enzyme showed a good thermostability, with optimal temperature in the 40– 50°C range and maintaining more than 40% of its maximal activity even at 10°C. Furthermore, Ant laccase was salt- and organic solvent-tolerant, paving the way for its use in harsh conditions. To our knowledge, this is the first report concerning the characterization of a thermo- and halo-tolerant laccase isolated from a marine Antarctic bacterium. Article in Journal/Newspaper Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea The University of Milan: Archivio Istituzionale della Ricerca (AIR) Antarctic Antarctic Ocean Ross Sea Terra Nova Bay The Antarctic Frontiers in Microbiology 14 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Settore BIO/10 - Biochimica |
spellingShingle |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Settore BIO/10 - Biochimica Bisaccia, Melissa Binda, Elisa Rosini, Elena Caruso, Gabriella Dell'Acqua, Ombretta Azzaro, Maurizio Laganà, Pasqualina Tedeschi, Gabriella Maffioli, Elisa M. Pollegioni, Loredano Marinelli, Flavia A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
topic_facet |
Antarctica laccase Halomonas sp marine biofilm cold-adaptation extremophile bacteria Settore BIO/10 - Biochimica |
description |
Microbial communities inhabiting the Antarctic Ocean show psychrophilic and halophilic adaptations conferring interesting properties to the enzymes they produce, which could be exploited in biotechnology and bioremediation processes. Use of cold- and salt-tolerant enzymes allows to limit costs, reduce contaminations, and minimize pretreatment steps. Here, we report on the screening of 186 morphologically diverse microorganisms isolated from marine biofilms and water samples collected in Terra Nova Bay (Ross Sea, Antarctica) for the identification of new laccase activities. After primary screening, 13.4 and 10.8% of the isolates were identified for the ability to oxidize 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and the dye azure B, respectively. Amongst them, the marine Halomonas sp. strain M68 showed the highest activity. Production of its laccase-like activity increased six-fold when copper was added to culture medium. Enzymatic activity-guided separation coupled with mass spectrometry identified this intracellular laccase-like protein (named Ant laccase) as belonging to the copper resistance system multicopper oxidase family. Ant laccase oxidized ABTS and 2,6-dimethoxy phenol, working better at acidic pHs The enzyme showed a good thermostability, with optimal temperature in the 40– 50°C range and maintaining more than 40% of its maximal activity even at 10°C. Furthermore, Ant laccase was salt- and organic solvent-tolerant, paving the way for its use in harsh conditions. To our knowledge, this is the first report concerning the characterization of a thermo- and halo-tolerant laccase isolated from a marine Antarctic bacterium. |
author2 |
M. Bisaccia E. Binda E. Rosini G. Caruso O. Dell'Acqua M. Azzaro P. Laganà G. Tedeschi E.M. Maffioli L. Pollegioni F. Marinelli |
format |
Article in Journal/Newspaper |
author |
Bisaccia, Melissa Binda, Elisa Rosini, Elena Caruso, Gabriella Dell'Acqua, Ombretta Azzaro, Maurizio Laganà, Pasqualina Tedeschi, Gabriella Maffioli, Elisa M. Pollegioni, Loredano Marinelli, Flavia |
author_facet |
Bisaccia, Melissa Binda, Elisa Rosini, Elena Caruso, Gabriella Dell'Acqua, Ombretta Azzaro, Maurizio Laganà, Pasqualina Tedeschi, Gabriella Maffioli, Elisa M. Pollegioni, Loredano Marinelli, Flavia |
author_sort |
Bisaccia, Melissa |
title |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_short |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_full |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_fullStr |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_full_unstemmed |
A novel promising laccase from the psychrotolerant and halotolerant Antarctic marine Halomonas sp. M68 strain |
title_sort |
novel promising laccase from the psychrotolerant and halotolerant antarctic marine halomonas sp. m68 strain |
publisher |
Frontiers Research Foundation |
publishDate |
2023 |
url |
https://hdl.handle.net/2434/954240 https://doi.org/10.3389/fmicb.2023.1078382 |
geographic |
Antarctic Antarctic Ocean Ross Sea Terra Nova Bay The Antarctic |
geographic_facet |
Antarctic Antarctic Ocean Ross Sea Terra Nova Bay The Antarctic |
genre |
Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctic Ocean Antarctica Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000937648000001 volume:14 firstpage:1 lastpage:13 numberofpages:13 journal:FRONTIERS IN MICROBIOLOGY https://hdl.handle.net/2434/954240 doi:10.3389/fmicb.2023.1078382 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85148762435 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2023.1078382 |
container_title |
Frontiers in Microbiology |
container_volume |
14 |
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1789959094778462208 |