Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species
Anhydrobiosis, a peculiar adaptive strategy existing in nature, is a reversible capability of organisms to tolerate a severe loss of their body water when their surrounding habitat is drying out. In the anhydrobiotic state, an organism lacks all dynamic features of living beings since an ongoing met...
Published in: | Life |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/2434/945172 https://doi.org/10.3390/life12060817 |
id |
ftunivmilanoair:oai:air.unimi.it:2434/945172 |
---|---|
record_format |
openpolar |
spelling |
ftunivmilanoair:oai:air.unimi.it:2434/945172 2024-02-04T09:52:16+01:00 Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species Ilaria Giovannini Paola Antonia Corsetto Tiziana Altiero Gigliola Montorfano Roberto Guidetti Angela Maria Rizzo Lorena Rebecchi I. Giovannini P.A. Corsetto T. Altiero G. Montorfano R. Guidetti A.M. Rizzo L. Rebecchi 2022 https://hdl.handle.net/2434/945172 https://doi.org/10.3390/life12060817 eng eng MDPI info:eu-repo/semantics/altIdentifier/pmid/35743848 info:eu-repo/semantics/altIdentifier/wos/WOS:000815907200001 volume:12 issue:6 firstpage:1 lastpage:13 numberofpages:13 journal:LIFE https://hdl.handle.net/2434/945172 doi:10.3390/life12060817 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85131679585 info:eu-repo/semantics/openAccess ROS anhydrobiosi desiccation stre scavenging enzyme tardigrades Settore BIO/10 - Biochimica info:eu-repo/semantics/article 2022 ftunivmilanoair https://doi.org/10.3390/life12060817 2024-01-09T23:15:02Z Anhydrobiosis, a peculiar adaptive strategy existing in nature, is a reversible capability of organisms to tolerate a severe loss of their body water when their surrounding habitat is drying out. In the anhydrobiotic state, an organism lacks all dynamic features of living beings since an ongoing metabolism is absent. The depletion of water in the anhydrobiotic state increases the ionic concentration and the production of reactive oxygen species (ROS). An imbalance between the increased production of ROS and the limited action of antioxidant defences is a source of biomolecular damage and can lead to oxidative stress. The deleterious effects of oxidative stress were demonstrated in anhydrobiotic unicellular and multicellular organisms, which counteract the effects using efficient antioxidant machinery, mainly represented by ROS scavenger enzymes. To gain insights into the dynamics of antioxidant patterns during the kinetics of the anhydrobiosis of two tardigrade species, Paramacrobiotus spatialis and Acutuncus antarcticus, we investigated the activity of enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase) and the amount of non-enzymatic antioxidants (glutathione) in the course of rehydration. In P. spatialis, the activity of catalase increases during dehydration and decreases during rehydration, whereas in A. antarcticus, the activity of superoxide dismutase decreases during desiccation and increases during rehydration. Genomic varieties, different habitats and geographical regions, different diets, and diverse evolutionary lineages may have led to the specialization of antioxidant strategies in the two species. Article in Journal/Newspaper Acutuncus antarcticus Antarc* antarcticus Tardigrade The University of Milan: Archivio Istituzionale della Ricerca (AIR) Life 12 6 817 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
ROS anhydrobiosi desiccation stre scavenging enzyme tardigrades Settore BIO/10 - Biochimica |
spellingShingle |
ROS anhydrobiosi desiccation stre scavenging enzyme tardigrades Settore BIO/10 - Biochimica Ilaria Giovannini Paola Antonia Corsetto Tiziana Altiero Gigliola Montorfano Roberto Guidetti Angela Maria Rizzo Lorena Rebecchi Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
topic_facet |
ROS anhydrobiosi desiccation stre scavenging enzyme tardigrades Settore BIO/10 - Biochimica |
description |
Anhydrobiosis, a peculiar adaptive strategy existing in nature, is a reversible capability of organisms to tolerate a severe loss of their body water when their surrounding habitat is drying out. In the anhydrobiotic state, an organism lacks all dynamic features of living beings since an ongoing metabolism is absent. The depletion of water in the anhydrobiotic state increases the ionic concentration and the production of reactive oxygen species (ROS). An imbalance between the increased production of ROS and the limited action of antioxidant defences is a source of biomolecular damage and can lead to oxidative stress. The deleterious effects of oxidative stress were demonstrated in anhydrobiotic unicellular and multicellular organisms, which counteract the effects using efficient antioxidant machinery, mainly represented by ROS scavenger enzymes. To gain insights into the dynamics of antioxidant patterns during the kinetics of the anhydrobiosis of two tardigrade species, Paramacrobiotus spatialis and Acutuncus antarcticus, we investigated the activity of enzymatic antioxidants (catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase) and the amount of non-enzymatic antioxidants (glutathione) in the course of rehydration. In P. spatialis, the activity of catalase increases during dehydration and decreases during rehydration, whereas in A. antarcticus, the activity of superoxide dismutase decreases during desiccation and increases during rehydration. Genomic varieties, different habitats and geographical regions, different diets, and diverse evolutionary lineages may have led to the specialization of antioxidant strategies in the two species. |
author2 |
I. Giovannini P.A. Corsetto T. Altiero G. Montorfano R. Guidetti A.M. Rizzo L. Rebecchi |
format |
Article in Journal/Newspaper |
author |
Ilaria Giovannini Paola Antonia Corsetto Tiziana Altiero Gigliola Montorfano Roberto Guidetti Angela Maria Rizzo Lorena Rebecchi |
author_facet |
Ilaria Giovannini Paola Antonia Corsetto Tiziana Altiero Gigliola Montorfano Roberto Guidetti Angela Maria Rizzo Lorena Rebecchi |
author_sort |
Ilaria Giovannini |
title |
Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
title_short |
Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
title_full |
Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
title_fullStr |
Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
title_full_unstemmed |
Antioxidant Response during the Kinetics of Anhydrobiosis in Two Eutardigrade Species |
title_sort |
antioxidant response during the kinetics of anhydrobiosis in two eutardigrade species |
publisher |
MDPI |
publishDate |
2022 |
url |
https://hdl.handle.net/2434/945172 https://doi.org/10.3390/life12060817 |
genre |
Acutuncus antarcticus Antarc* antarcticus Tardigrade |
genre_facet |
Acutuncus antarcticus Antarc* antarcticus Tardigrade |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/35743848 info:eu-repo/semantics/altIdentifier/wos/WOS:000815907200001 volume:12 issue:6 firstpage:1 lastpage:13 numberofpages:13 journal:LIFE https://hdl.handle.net/2434/945172 doi:10.3390/life12060817 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85131679585 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/life12060817 |
container_title |
Life |
container_volume |
12 |
container_issue |
6 |
container_start_page |
817 |
_version_ |
1789964198851117056 |