Rearing environment during the endogenous feeding stage of Acipenser baerii
The aim of this study was to evaluate behaviour, growth, lipid composition, muscle development, and stress status of Siberian sturgeon larvae reared with two types of substrate: Bioballs1 (BB1) and Bioballs2 (BB2), when compared to no substrate (CTR). Sampling points were: hatching (T0), schooling (...
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Online Access: | http://hdl.handle.net/2434/938553 https://doi.org/10.3390/ani12172205 |
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ftunivmilanoair:oai:air.unimi.it:2434/938553 2024-01-28T09:57:51+01:00 Rearing environment during the endogenous feeding stage of Acipenser baerii Lucia Aidos Alessandra Cafiso Annalaura Lopez Mauro Vasconi Luisa M. P. Valente Chiara Bazzocchi Alessia Di Giancamillo A. Lucia A. Cafiso A. Lopez M. Vasconi L.M.P. Valente C. Bazzocchi A. DI GIANCAMILLO 2022-09 http://hdl.handle.net/2434/938553 https://doi.org/10.3390/ani12172205 eng eng MDPI info:eu-repo/semantics/altIdentifier/pmid/36077925 info:eu-repo/semantics/altIdentifier/wos/WOS:000850959700001 volume:12 issue:17 firstpage:1 lastpage:18 numberofpages:18 journal:ANIMALS http://hdl.handle.net/2434/938553 doi:10.3390/ani12172205 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85137765655 info:eu-repo/semantics/openAccess Acipenser baerii substrate muscle development swimming behaviour histometry gene expression Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali Settore VET/01 - Anatomia degli Animali Domestici Settore AGR/20 - Zoocolture info:eu-repo/semantics/article 2022 ftunivmilanoair https://doi.org/10.3390/ani12172205 2024-01-02T23:15:10Z The aim of this study was to evaluate behaviour, growth, lipid composition, muscle development, and stress status of Siberian sturgeon larvae reared with two types of substrate: Bioballs1 (BB1) and Bioballs2 (BB2), when compared to no substrate (CTR). Sampling points were: hatching (T0), schooling (T1), and yolk-sac full absorption (T2). BB1 larvae were less active and showed no schooling behaviour. At T1 and at T2, BB1 larvae showed a significantly higher weight and total length than larvae reared in either CTR or BB2 (p < 0.05). The lipid content of larvae decreased over time, with little relevant differences between groups. At T2, total muscle area, slow muscle area and fast muscle area were significantly higher in larvae reared in BB1 (p < 0.05). No significant differences in muscle proliferation were found between groups. Real Time PCR was used for evaluating the relative expression of a pool of genes: myod, myog, mrf4, igf2, hsp70, hsp90a, hsp90b, and glut2. The expression of these genes did not seem to be much affected by the type of rearing substrate, except for myog and hsp70 at T1, which was greater in BB2 larvae. Our data suggest that the presence of a substrate during this developmental period seems to have positive effects but further studies would be necessary during the exogenous feeding stage. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon The University of Milan: Archivio Istituzionale della Ricerca (AIR) Animals 12 17 2205 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Acipenser baerii substrate muscle development swimming behaviour histometry gene expression Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali Settore VET/01 - Anatomia degli Animali Domestici Settore AGR/20 - Zoocolture |
spellingShingle |
Acipenser baerii substrate muscle development swimming behaviour histometry gene expression Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali Settore VET/01 - Anatomia degli Animali Domestici Settore AGR/20 - Zoocolture Lucia Aidos Alessandra Cafiso Annalaura Lopez Mauro Vasconi Luisa M. P. Valente Chiara Bazzocchi Alessia Di Giancamillo Rearing environment during the endogenous feeding stage of Acipenser baerii |
topic_facet |
Acipenser baerii substrate muscle development swimming behaviour histometry gene expression Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali Settore VET/01 - Anatomia degli Animali Domestici Settore AGR/20 - Zoocolture |
description |
The aim of this study was to evaluate behaviour, growth, lipid composition, muscle development, and stress status of Siberian sturgeon larvae reared with two types of substrate: Bioballs1 (BB1) and Bioballs2 (BB2), when compared to no substrate (CTR). Sampling points were: hatching (T0), schooling (T1), and yolk-sac full absorption (T2). BB1 larvae were less active and showed no schooling behaviour. At T1 and at T2, BB1 larvae showed a significantly higher weight and total length than larvae reared in either CTR or BB2 (p < 0.05). The lipid content of larvae decreased over time, with little relevant differences between groups. At T2, total muscle area, slow muscle area and fast muscle area were significantly higher in larvae reared in BB1 (p < 0.05). No significant differences in muscle proliferation were found between groups. Real Time PCR was used for evaluating the relative expression of a pool of genes: myod, myog, mrf4, igf2, hsp70, hsp90a, hsp90b, and glut2. The expression of these genes did not seem to be much affected by the type of rearing substrate, except for myog and hsp70 at T1, which was greater in BB2 larvae. Our data suggest that the presence of a substrate during this developmental period seems to have positive effects but further studies would be necessary during the exogenous feeding stage. |
author2 |
A. Lucia A. Cafiso A. Lopez M. Vasconi L.M.P. Valente C. Bazzocchi A. DI GIANCAMILLO |
format |
Article in Journal/Newspaper |
author |
Lucia Aidos Alessandra Cafiso Annalaura Lopez Mauro Vasconi Luisa M. P. Valente Chiara Bazzocchi Alessia Di Giancamillo |
author_facet |
Lucia Aidos Alessandra Cafiso Annalaura Lopez Mauro Vasconi Luisa M. P. Valente Chiara Bazzocchi Alessia Di Giancamillo |
author_sort |
Lucia Aidos |
title |
Rearing environment during the endogenous feeding stage of Acipenser baerii |
title_short |
Rearing environment during the endogenous feeding stage of Acipenser baerii |
title_full |
Rearing environment during the endogenous feeding stage of Acipenser baerii |
title_fullStr |
Rearing environment during the endogenous feeding stage of Acipenser baerii |
title_full_unstemmed |
Rearing environment during the endogenous feeding stage of Acipenser baerii |
title_sort |
rearing environment during the endogenous feeding stage of acipenser baerii |
publisher |
MDPI |
publishDate |
2022 |
url |
http://hdl.handle.net/2434/938553 https://doi.org/10.3390/ani12172205 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/36077925 info:eu-repo/semantics/altIdentifier/wos/WOS:000850959700001 volume:12 issue:17 firstpage:1 lastpage:18 numberofpages:18 journal:ANIMALS http://hdl.handle.net/2434/938553 doi:10.3390/ani12172205 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85137765655 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/ani12172205 |
container_title |
Animals |
container_volume |
12 |
container_issue |
17 |
container_start_page |
2205 |
_version_ |
1789326877901455360 |