Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis)
Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existe...
Published in: | Viruses |
---|---|
Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI
2019
|
Subjects: | |
Online Access: | https://hdl.handle.net/2434/922112 https://doi.org/10.3390/v11040363 |
id |
ftunivmilanoair:oai:air.unimi.it:2434/922112 |
---|---|
record_format |
openpolar |
spelling |
ftunivmilanoair:oai:air.unimi.it:2434/922112 2024-04-21T08:07:06+00:00 Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) Hendrikse L. D. Kambli A. Kayko C. Canuti M. Rodrigues B. Stevens B. Vashon J. Lang A. S. Needle D. B. Troyer R. M. L.D. Hendrikse A. Kambli C. Kayko M. Canuti B. Rodrigue B. Steven J. Vashon A.S. Lang D.B. Needle R.M. Troyer 2019-04 https://hdl.handle.net/2434/922112 https://doi.org/10.3390/v11040363 eng eng MDPI info:eu-repo/semantics/altIdentifier/pmid/31010021 info:eu-repo/semantics/altIdentifier/wos/WOS:000467310600060 volume:11 issue:4 firstpage:1 lastpage:15 numberofpages:15 journal:VIRUSES https://hdl.handle.net/2434/922112 doi:10.3390/v11040363 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85065055851 info:eu-repo/semantics/openAccess Cat DNA polymerase Felid Glycoprotein B Herpesviru Lynx Percaviru Phylogenetic Viru Animal Canada DNA Polymerase III DNA Viral Female Gammaherpesvirinae Male Phylogeny Settore BIO/19 - Microbiologia Generale info:eu-repo/semantics/article 2019 ftunivmilanoair https://doi.org/10.3390/v11040363 2024-03-27T02:08:38Z Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existence of an unidentified GHV in lynx. Using degenerate nested and subsequently virus-specific PCR, we amplified and sequenced 3.4 kb of DNA from a novel GHV in lynx, which we named Lynx canadensis gammaherpesvirus 1 (LcaGHV1). Phylogenetic analysis determined that LcaGHV1 is a distinct GHV species belonging to the genus Percavirus. We then estimated the prevalence of LcaGHV1 in lynx by developing a PCR-based assay and detected LcaGHV1 DNA in 36% (95% CI: 22–53%) of lynx spleen DNA samples from Maine, USA and 17% (95% CI: 8–31%) from Newfoundland, Canada. The LcaGHV1 DNA sequences from Maine and Newfoundland lynx were nearly identical to each other (two nucleotide substitutions in 3.4 kb), suggesting that the unique lynx subspecies present on the island of Newfoundland (Lynx canadensis subsolanus) is infected with virus that very closely resembles virus found in mainland lynx. The potential ecologic and pathologic consequences of this novel virus for Canada lynx populations warrant further study. Article in Journal/Newspaper Newfoundland Lynx The University of Milan: Archivio Istituzionale della Ricerca (AIR) Viruses 11 4 363 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Cat DNA polymerase Felid Glycoprotein B Herpesviru Lynx Percaviru Phylogenetic Viru Animal Canada DNA Polymerase III DNA Viral Female Gammaherpesvirinae Male Phylogeny Settore BIO/19 - Microbiologia Generale |
spellingShingle |
Cat DNA polymerase Felid Glycoprotein B Herpesviru Lynx Percaviru Phylogenetic Viru Animal Canada DNA Polymerase III DNA Viral Female Gammaherpesvirinae Male Phylogeny Settore BIO/19 - Microbiologia Generale Hendrikse L. D. Kambli A. Kayko C. Canuti M. Rodrigues B. Stevens B. Vashon J. Lang A. S. Needle D. B. Troyer R. M. Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
topic_facet |
Cat DNA polymerase Felid Glycoprotein B Herpesviru Lynx Percaviru Phylogenetic Viru Animal Canada DNA Polymerase III DNA Viral Female Gammaherpesvirinae Male Phylogeny Settore BIO/19 - Microbiologia Generale |
description |
Gammaherpesviruses (GHVs) infect many animal species and are associated with lymphoproliferative disorders in some. Previously, we identified several novel GHVs in North American felids; however, a GHV had never been identified in Canada lynx (Lynx canadensis). We, therefore, hypothesized the existence of an unidentified GHV in lynx. Using degenerate nested and subsequently virus-specific PCR, we amplified and sequenced 3.4 kb of DNA from a novel GHV in lynx, which we named Lynx canadensis gammaherpesvirus 1 (LcaGHV1). Phylogenetic analysis determined that LcaGHV1 is a distinct GHV species belonging to the genus Percavirus. We then estimated the prevalence of LcaGHV1 in lynx by developing a PCR-based assay and detected LcaGHV1 DNA in 36% (95% CI: 22–53%) of lynx spleen DNA samples from Maine, USA and 17% (95% CI: 8–31%) from Newfoundland, Canada. The LcaGHV1 DNA sequences from Maine and Newfoundland lynx were nearly identical to each other (two nucleotide substitutions in 3.4 kb), suggesting that the unique lynx subspecies present on the island of Newfoundland (Lynx canadensis subsolanus) is infected with virus that very closely resembles virus found in mainland lynx. The potential ecologic and pathologic consequences of this novel virus for Canada lynx populations warrant further study. |
author2 |
L.D. Hendrikse A. Kambli C. Kayko M. Canuti B. Rodrigue B. Steven J. Vashon A.S. Lang D.B. Needle R.M. Troyer |
format |
Article in Journal/Newspaper |
author |
Hendrikse L. D. Kambli A. Kayko C. Canuti M. Rodrigues B. Stevens B. Vashon J. Lang A. S. Needle D. B. Troyer R. M. |
author_facet |
Hendrikse L. D. Kambli A. Kayko C. Canuti M. Rodrigues B. Stevens B. Vashon J. Lang A. S. Needle D. B. Troyer R. M. |
author_sort |
Hendrikse L. D. |
title |
Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_short |
Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_full |
Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_fullStr |
Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_full_unstemmed |
Identification of a novel gammaherpesvirus in Canada lynx (Lynx canadensis) |
title_sort |
identification of a novel gammaherpesvirus in canada lynx (lynx canadensis) |
publisher |
MDPI |
publishDate |
2019 |
url |
https://hdl.handle.net/2434/922112 https://doi.org/10.3390/v11040363 |
genre |
Newfoundland Lynx |
genre_facet |
Newfoundland Lynx |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/31010021 info:eu-repo/semantics/altIdentifier/wos/WOS:000467310600060 volume:11 issue:4 firstpage:1 lastpage:15 numberofpages:15 journal:VIRUSES https://hdl.handle.net/2434/922112 doi:10.3390/v11040363 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85065055851 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/v11040363 |
container_title |
Viruses |
container_volume |
11 |
container_issue |
4 |
container_start_page |
363 |
_version_ |
1796946728544370688 |