Diversity and small-scale distribution of microbial communities in patterned grounds
Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studie...
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ftunivmilanoair:oai:air.unimi.it:2434/874653 2024-01-28T10:08:36+01:00 Diversity and small-scale distribution of microbial communities in patterned grounds Mania Ilaria Freppaz Michele D'Amico Michele Gorra Roberta I. Mania M. Freppaz M. D'Amico R. Gorra 2015-06 http://hdl.handle.net/2434/874653 eng eng Symposium BAGECO-Bacterial genetics and ecology : the microbial continuity across changing ecosystems http://hdl.handle.net/2434/874653 Patterned ground cryoturbation microbial communitie DGGE qPCR Settore AGR/14 - Pedologia Settore AGR/16 - Microbiologia Agraria info:eu-repo/semantics/conferenceObject 2015 ftunivmilanoair 2024-01-02T23:53:08Z Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studies described PGs morphology and their formative processes, microbiological aspects are yet greatly unexplored. Microbial communities that develop in these ecosystems may play important roles in nutrient availability, dynamics and stabilization and therefore in plant colonization and ecosystem evolution. In this study, we focused on circular patterned grounds showing a concentric textural sorting from four areas, characterized by different lithotypes, in the North-western Italian Alps. Samples were subjected to phylogenetic fingerprint by Denaturing Gradient Gel Electrophoresis (DGGE) followed by band sequencing. Moreover, abundance and distribution of bacterial, archaeal and fungal genetic markers and functional genes were measured by quantitative real-time PCR and correlated to chemical parameters. DGGE profiles revealed a relatively homogeneous community structure within circles, but also among different PGs, with several phylotypes previously observed in other PGs and cold environments. Only for Archaea stronger differences in community composition on small-scale were detectable. On a quantitative point of view, microbial populations showed a clear concentric distribution, correlated to C:N ratio, influenced by lithology of parent material and coherent with trends of physicochemical parameters within PG circles. These first results seem to suggest the importance of cryoturbation phenomenon in shaping microbial communities in seasonally frozen soils, at least in a quantitative way, although more detailed functional analysis are needed to make hypothesis about mechanisms involved. Conference Object permafrost The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
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The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Patterned ground cryoturbation microbial communitie DGGE qPCR Settore AGR/14 - Pedologia Settore AGR/16 - Microbiologia Agraria |
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Patterned ground cryoturbation microbial communitie DGGE qPCR Settore AGR/14 - Pedologia Settore AGR/16 - Microbiologia Agraria Mania Ilaria Freppaz Michele D'Amico Michele Gorra Roberta Diversity and small-scale distribution of microbial communities in patterned grounds |
topic_facet |
Patterned ground cryoturbation microbial communitie DGGE qPCR Settore AGR/14 - Pedologia Settore AGR/16 - Microbiologia Agraria |
description |
Patterned Grounds (PGs) are geometric features formed on permafrost soils as a result of cryoturbation: the mixing, heaving, and churning of soil that occurs during freeze-thaw cycles forms stripes, circles, polygons and nets with and without visible surface textural sorting. Although several studies described PGs morphology and their formative processes, microbiological aspects are yet greatly unexplored. Microbial communities that develop in these ecosystems may play important roles in nutrient availability, dynamics and stabilization and therefore in plant colonization and ecosystem evolution. In this study, we focused on circular patterned grounds showing a concentric textural sorting from four areas, characterized by different lithotypes, in the North-western Italian Alps. Samples were subjected to phylogenetic fingerprint by Denaturing Gradient Gel Electrophoresis (DGGE) followed by band sequencing. Moreover, abundance and distribution of bacterial, archaeal and fungal genetic markers and functional genes were measured by quantitative real-time PCR and correlated to chemical parameters. DGGE profiles revealed a relatively homogeneous community structure within circles, but also among different PGs, with several phylotypes previously observed in other PGs and cold environments. Only for Archaea stronger differences in community composition on small-scale were detectable. On a quantitative point of view, microbial populations showed a clear concentric distribution, correlated to C:N ratio, influenced by lithology of parent material and coherent with trends of physicochemical parameters within PG circles. These first results seem to suggest the importance of cryoturbation phenomenon in shaping microbial communities in seasonally frozen soils, at least in a quantitative way, although more detailed functional analysis are needed to make hypothesis about mechanisms involved. |
author2 |
I. Mania M. Freppaz M. D'Amico R. Gorra |
format |
Conference Object |
author |
Mania Ilaria Freppaz Michele D'Amico Michele Gorra Roberta |
author_facet |
Mania Ilaria Freppaz Michele D'Amico Michele Gorra Roberta |
author_sort |
Mania Ilaria |
title |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_short |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_full |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_fullStr |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_full_unstemmed |
Diversity and small-scale distribution of microbial communities in patterned grounds |
title_sort |
diversity and small-scale distribution of microbial communities in patterned grounds |
publishDate |
2015 |
url |
http://hdl.handle.net/2434/874653 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
Symposium BAGECO-Bacterial genetics and ecology : the microbial continuity across changing ecosystems http://hdl.handle.net/2434/874653 |
_version_ |
1789337518817148928 |