Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit
Background: Persistent organic pollutants (POPs) may influence epigenetic mechanisms; therefore, they could affect chromosomal stability and gene expression. DNA methylation, an epigenetic mechanism, has been associated with cancer initiation and progression. Greenlandic Inuit have some of the highe...
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Online Access: | http://hdl.handle.net/2434/70478 https://doi.org/10.1289/ehp.11338 |
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ftunivmilanoair:oai:air.unimi.it:2434/70478 2024-02-11T09:59:54+01:00 Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit J. A. Rusiecki L. E. Moore E. C. Bonefeld Jorgensen A. Baccarelli V. Bollati L. Tarantini J.A. Rusiecki A. Baccarelli V. Bollati L. Tarantini L.E. Moore E.C. Bonefeld-Jorgensen 2008 http://hdl.handle.net/2434/70478 https://doi.org/10.1289/ehp.11338 eng eng info:eu-repo/semantics/altIdentifier/pmid/19057709 info:eu-repo/semantics/altIdentifier/wos/WOS:000260521500030 volume:116 issue:11 firstpage:1547 lastpage:1552 journal:ENVIRONMENTAL HEALTH PERSPECTIVES http://hdl.handle.net/2434/70478 doi:10.1289/ehp.11338 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-58849158772 DNA methylation Global methylation Greenland Hypomethylation Inuit Organochlorine PCB Persistent organic pollutant Pesticide Polychlorinated biphenyl POP Serum Settore MED/44 - Medicina del Lavoro info:eu-repo/semantics/article 2008 ftunivmilanoair https://doi.org/10.1289/ehp.11338 2024-01-23T23:18:30Z Background: Persistent organic pollutants (POPs) may influence epigenetic mechanisms; therefore, they could affect chromosomal stability and gene expression. DNA methylation, an epigenetic mechanism, has been associated with cancer initiation and progression. Greenlandic Inuit have some of the highest reported POP levels worldwide. Objective: Our aim in this study was to evaluate the relationship between plasma POPs concentrations and global DNA methylation (percent 5-methylcytosine) in DNA extracted from blood samples from 70 Greenlandic Inuit. Blood samples were collected under the Arctic Monitoring and Assessment Program and previously analyzed for a battery of POPs. Methods: We used pyrosequencing to estimate global DNA methylation via Alu and LINE-1 assays of bisulfite-treated DNA. We investigated correlations between plasma POP concentrations and global DNA methylation via correlation coefficients and linear regression analyses. Results: We found inverse correlations between percents methylcytosine and many of the POP concentrations measured. Linear regressions, adjusting for age and cigarette smoking, showed statistically significant inverse linear relationships mainly for the Alu assay for p,p'-DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane; β = -0.26), p,p'-DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene; β = -0.38], β-hexachlorocyclohexane (β = -0.48), oxychlordane (β = -0.32), α-chlordane (β = -0.75), mirex (β = -0.27), sum of polychlorinated biphenyls (β = -0.56), and sum of all POPs (β = -0.48). Linear regressions for the LINE-1 assay showed β estimates of similar magnitudes to those using the Alu assay, however, none was statistically significant. Conclusions: This is the first study to investigate environmental exposure to POPs and DNA methylation levels in a human population. Global methylation levels were inversely associated with blood plasma levels for several POPs and merit further investigation. Article in Journal/Newspaper Arctic monitoring and assessment program Arctic Greenland greenlandic inuit The University of Milan: Archivio Istituzionale della Ricerca (AIR) Arctic Greenland Environmental Health Perspectives 116 11 1547 1552 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
DNA methylation Global methylation Greenland Hypomethylation Inuit Organochlorine PCB Persistent organic pollutant Pesticide Polychlorinated biphenyl POP Serum Settore MED/44 - Medicina del Lavoro |
spellingShingle |
DNA methylation Global methylation Greenland Hypomethylation Inuit Organochlorine PCB Persistent organic pollutant Pesticide Polychlorinated biphenyl POP Serum Settore MED/44 - Medicina del Lavoro J. A. Rusiecki L. E. Moore E. C. Bonefeld Jorgensen A. Baccarelli V. Bollati L. Tarantini Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
topic_facet |
DNA methylation Global methylation Greenland Hypomethylation Inuit Organochlorine PCB Persistent organic pollutant Pesticide Polychlorinated biphenyl POP Serum Settore MED/44 - Medicina del Lavoro |
description |
Background: Persistent organic pollutants (POPs) may influence epigenetic mechanisms; therefore, they could affect chromosomal stability and gene expression. DNA methylation, an epigenetic mechanism, has been associated with cancer initiation and progression. Greenlandic Inuit have some of the highest reported POP levels worldwide. Objective: Our aim in this study was to evaluate the relationship between plasma POPs concentrations and global DNA methylation (percent 5-methylcytosine) in DNA extracted from blood samples from 70 Greenlandic Inuit. Blood samples were collected under the Arctic Monitoring and Assessment Program and previously analyzed for a battery of POPs. Methods: We used pyrosequencing to estimate global DNA methylation via Alu and LINE-1 assays of bisulfite-treated DNA. We investigated correlations between plasma POP concentrations and global DNA methylation via correlation coefficients and linear regression analyses. Results: We found inverse correlations between percents methylcytosine and many of the POP concentrations measured. Linear regressions, adjusting for age and cigarette smoking, showed statistically significant inverse linear relationships mainly for the Alu assay for p,p'-DDT (1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane; β = -0.26), p,p'-DDE [1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene; β = -0.38], β-hexachlorocyclohexane (β = -0.48), oxychlordane (β = -0.32), α-chlordane (β = -0.75), mirex (β = -0.27), sum of polychlorinated biphenyls (β = -0.56), and sum of all POPs (β = -0.48). Linear regressions for the LINE-1 assay showed β estimates of similar magnitudes to those using the Alu assay, however, none was statistically significant. Conclusions: This is the first study to investigate environmental exposure to POPs and DNA methylation levels in a human population. Global methylation levels were inversely associated with blood plasma levels for several POPs and merit further investigation. |
author2 |
J.A. Rusiecki A. Baccarelli V. Bollati L. Tarantini L.E. Moore E.C. Bonefeld-Jorgensen |
format |
Article in Journal/Newspaper |
author |
J. A. Rusiecki L. E. Moore E. C. Bonefeld Jorgensen A. Baccarelli V. Bollati L. Tarantini |
author_facet |
J. A. Rusiecki L. E. Moore E. C. Bonefeld Jorgensen A. Baccarelli V. Bollati L. Tarantini |
author_sort |
J. A. Rusiecki |
title |
Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
title_short |
Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
title_full |
Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
title_fullStr |
Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
title_full_unstemmed |
Global DNA hypomethylation is associated with high serum-persistent organic pollutants in Greenlandic Inuit |
title_sort |
global dna hypomethylation is associated with high serum-persistent organic pollutants in greenlandic inuit |
publishDate |
2008 |
url |
http://hdl.handle.net/2434/70478 https://doi.org/10.1289/ehp.11338 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic monitoring and assessment program Arctic Greenland greenlandic inuit |
genre_facet |
Arctic monitoring and assessment program Arctic Greenland greenlandic inuit |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/19057709 info:eu-repo/semantics/altIdentifier/wos/WOS:000260521500030 volume:116 issue:11 firstpage:1547 lastpage:1552 journal:ENVIRONMENTAL HEALTH PERSPECTIVES http://hdl.handle.net/2434/70478 doi:10.1289/ehp.11338 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-58849158772 |
op_doi |
https://doi.org/10.1289/ehp.11338 |
container_title |
Environmental Health Perspectives |
container_volume |
116 |
container_issue |
11 |
container_start_page |
1547 |
op_container_end_page |
1552 |
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1790595624934047744 |