Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758)
The aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (TNT) with liver biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758). Eels were exposed to 0.5, 1 and 2.5 mg/l nominal concentrations of TNT for 6 and 24 h. Modulation of CYP1A1, UDPGT a...
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ftunivmilanoair:oai:air.unimi.it:2434/653028 2024-02-04T09:52:54+01:00 Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A VALOTI, MASSIMO FOCARDI, SILVANO ETTORE C. DELLA TORRE I. Corsi A. Arukwe M. Valoti S.E. Focardi 2008 http://hdl.handle.net/2434/653028 https://doi.org/10.1016/j.ecoenv.2008.03.003 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000260275500026 volume:71 issue:3 firstpage:798 lastpage:805 numberofpages:8 journal:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://hdl.handle.net/2434/653028 doi:10.1016/j.ecoenv.2008.03.003 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-53249097926 2,4,6-trinitrotoluene Cytochrome P450 Biotransformation European eel Settore BIO/07 - Ecologia info:eu-repo/semantics/article 2008 ftunivmilanoair https://doi.org/10.1016/j.ecoenv.2008.03.003 2024-01-09T23:40:19Z The aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (TNT) with liver biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758). Eels were exposed to 0.5, 1 and 2.5 mg/l nominal concentrations of TNT for 6 and 24 h. Modulation of CYP1A1, UDPGT and GST genes was investigated by real-time PCR. Total CYP450 content, NADPH cytochrome c reductase activity, CYP1A and CYP2B-like activities, such as EROD, MROD and BROD, as well as GST and UDPGT activities, were measured by biochemical assays. An in vitro study was performed on EROD in order to evaluate catalytic modulation by TNT. No modulation of the CYP1A1 gene or protein was observed in TNT-exposed eels. On the other hand, a significant decline of EROD and MROD activities was observed in vivo. An increase in NADPH cyt c reductase, and phase II enzymes (UDPGT and GST) were observed at both gene expression and activity levels. The overall results indicated that TNT is a potential competitive inhibitor of CYP1A activities. A TNT metabolic pathway involving NADPH cyt c reductase and phase II enzymes is also suggested. Article in Journal/Newspaper Anguilla anguilla The University of Milan: Archivio Istituzionale della Ricerca (AIR) Ecotoxicology and Environmental Safety 71 3 798 805 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
2,4,6-trinitrotoluene Cytochrome P450 Biotransformation European eel Settore BIO/07 - Ecologia |
spellingShingle |
2,4,6-trinitrotoluene Cytochrome P450 Biotransformation European eel Settore BIO/07 - Ecologia DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A VALOTI, MASSIMO FOCARDI, SILVANO ETTORE Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
topic_facet |
2,4,6-trinitrotoluene Cytochrome P450 Biotransformation European eel Settore BIO/07 - Ecologia |
description |
The aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (TNT) with liver biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758). Eels were exposed to 0.5, 1 and 2.5 mg/l nominal concentrations of TNT for 6 and 24 h. Modulation of CYP1A1, UDPGT and GST genes was investigated by real-time PCR. Total CYP450 content, NADPH cytochrome c reductase activity, CYP1A and CYP2B-like activities, such as EROD, MROD and BROD, as well as GST and UDPGT activities, were measured by biochemical assays. An in vitro study was performed on EROD in order to evaluate catalytic modulation by TNT. No modulation of the CYP1A1 gene or protein was observed in TNT-exposed eels. On the other hand, a significant decline of EROD and MROD activities was observed in vivo. An increase in NADPH cyt c reductase, and phase II enzymes (UDPGT and GST) were observed at both gene expression and activity levels. The overall results indicated that TNT is a potential competitive inhibitor of CYP1A activities. A TNT metabolic pathway involving NADPH cyt c reductase and phase II enzymes is also suggested. |
author2 |
C. DELLA TORRE I. Corsi A. Arukwe M. Valoti S.E. Focardi |
format |
Article in Journal/Newspaper |
author |
DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A VALOTI, MASSIMO FOCARDI, SILVANO ETTORE |
author_facet |
DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A VALOTI, MASSIMO FOCARDI, SILVANO ETTORE |
author_sort |
DELLA TORRE, CAMILLA |
title |
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
title_short |
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full |
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
title_fullStr |
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full_unstemmed |
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758) |
title_sort |
interactions of 2,4,6-trinitrotoluene (tnt) with xenobiotic biotransformation system in european eel anguilla anguilla (linnaeus, 1758) |
publishDate |
2008 |
url |
http://hdl.handle.net/2434/653028 https://doi.org/10.1016/j.ecoenv.2008.03.003 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000260275500026 volume:71 issue:3 firstpage:798 lastpage:805 numberofpages:8 journal:ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://hdl.handle.net/2434/653028 doi:10.1016/j.ecoenv.2008.03.003 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-53249097926 |
op_doi |
https://doi.org/10.1016/j.ecoenv.2008.03.003 |
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Ecotoxicology and Environmental Safety |
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71 |
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3 |
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