Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758)
The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. E...
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Online Access: | http://hdl.handle.net/2434/652601 https://doi.org/10.1016/j.marenvres.2008.02.008 |
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ftunivmilanoair:oai:air.unimi.it:2434/652601 2024-02-11T09:55:29+01:00 Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A ALCARO L AMATO E FOCARDI, SILVANO ETTORE C. DELLA TORRE I. Corsi A. Arukwe L. Alcaro E. Amato S.E. Focardi 2008 http://hdl.handle.net/2434/652601 https://doi.org/10.1016/j.marenvres.2008.02.008 eng eng info:eu-repo/semantics/altIdentifier/pmid/18378298 info:eu-repo/semantics/altIdentifier/wos/WOS:000257575400005 International Symposium on Pollutant Responses in Marine Organisms (PRIMO 14) volume:66 issue:1 firstpage:9 lastpage:11 numberofpages:3 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/2434/652601 doi:10.1016/j.marenvres.2008.02.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-44749083437 info:eu-repo/semantics/closedAccess 2,4,6-trinitrotoluene CYP1A GST UDPGT European eel Settore BIO/07 - Ecologia info:eu-repo/semantics/article 2008 ftunivmilanoair https://doi.org/10.1016/j.marenvres.2008.02.008 2024-01-23T23:36:57Z The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. Expression of CYPIA, glutathione-S-transferase (pi-class; CST) and uridine-diphosphate glucuronosyltransferase (]family) (UDPGT) genes was investigated by RT-PCR, and 7-ethoxy- and 7-methoxyresorufln-0-deall :ylases (EROD, MROD), NADPH cyt c reductase (NADPH red), UDPGT and CST enzyme activities were measured by biochemical assays. An in vitro study was also performed, measuring only EROD activity. TNT exposure produced no modulation of CYPlA transcript expression while a significant inhibition of EROD enzyme activity was observed and confirmed in vitro. UDPGT transcript increased closedependently only at 6 h while the UDPGT activity tended to increase dose-dependently at 24 h. CST gene expression increased after 24 h and significant increases of CST activity were observed both at 6 and 24 h only at the highest TNT concentration. An increase of NADPH red activity was observed at 24 h. Our results seem to indicate an inhibitory effect of TNT on CYPI A-dependent catalytic activities and a possible involvement of phase 11 enzymes as well as NADPH red in TNT metabolism in eels. Article in Journal/Newspaper Anguilla anguilla European eel The University of Milan: Archivio Istituzionale della Ricerca (AIR) Marine Environmental Research 66 1 9 11 |
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Open Polar |
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The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
2,4,6-trinitrotoluene CYP1A GST UDPGT European eel Settore BIO/07 - Ecologia |
spellingShingle |
2,4,6-trinitrotoluene CYP1A GST UDPGT European eel Settore BIO/07 - Ecologia DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A ALCARO L AMATO E FOCARDI, SILVANO ETTORE Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
topic_facet |
2,4,6-trinitrotoluene CYP1A GST UDPGT European eel Settore BIO/07 - Ecologia |
description |
The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. Expression of CYPIA, glutathione-S-transferase (pi-class; CST) and uridine-diphosphate glucuronosyltransferase (]family) (UDPGT) genes was investigated by RT-PCR, and 7-ethoxy- and 7-methoxyresorufln-0-deall :ylases (EROD, MROD), NADPH cyt c reductase (NADPH red), UDPGT and CST enzyme activities were measured by biochemical assays. An in vitro study was also performed, measuring only EROD activity. TNT exposure produced no modulation of CYPlA transcript expression while a significant inhibition of EROD enzyme activity was observed and confirmed in vitro. UDPGT transcript increased closedependently only at 6 h while the UDPGT activity tended to increase dose-dependently at 24 h. CST gene expression increased after 24 h and significant increases of CST activity were observed both at 6 and 24 h only at the highest TNT concentration. An increase of NADPH red activity was observed at 24 h. Our results seem to indicate an inhibitory effect of TNT on CYPI A-dependent catalytic activities and a possible involvement of phase 11 enzymes as well as NADPH red in TNT metabolism in eels. |
author2 |
C. DELLA TORRE I. Corsi A. Arukwe L. Alcaro E. Amato S.E. Focardi |
format |
Article in Journal/Newspaper |
author |
DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A ALCARO L AMATO E FOCARDI, SILVANO ETTORE |
author_facet |
DELLA TORRE, CAMILLA CORSI, ILARIA ARUKWE A ALCARO L AMATO E FOCARDI, SILVANO ETTORE |
author_sort |
DELLA TORRE, CAMILLA |
title |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_short |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_fullStr |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full_unstemmed |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_sort |
effects of 2,4,6-trinitrotoluene (tnt) on phase i and phase ii biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758) |
publishDate |
2008 |
url |
http://hdl.handle.net/2434/652601 https://doi.org/10.1016/j.marenvres.2008.02.008 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/18378298 info:eu-repo/semantics/altIdentifier/wos/WOS:000257575400005 International Symposium on Pollutant Responses in Marine Organisms (PRIMO 14) volume:66 issue:1 firstpage:9 lastpage:11 numberofpages:3 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/2434/652601 doi:10.1016/j.marenvres.2008.02.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-44749083437 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.marenvres.2008.02.008 |
container_title |
Marine Environmental Research |
container_volume |
66 |
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1 |
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9 |
op_container_end_page |
11 |
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1790596620810715136 |