Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758)
The aim of the present study is to investigate the endocrine disrupting potential of the explosive 2,4,6-trinitrotoluene (TNT) on marine organisms using the European eel (Anguilla anguilla Linnaeus, 1758) as model fish species. Crucial brain steroidogenic parameters such as the estrogen receptors (E...
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Online Access: | http://hdl.handle.net/2434/652588 https://doi.org/10.1080/02757540801966272 |
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ftunivmilanoair:oai:air.unimi.it:2434/652588 2024-02-11T09:55:30+01:00 Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) DELLA TORRE, CAMILLA CORSI, ILARIA FOCARDI, SILVANO ETTORE ARUKWE A. C. DELLA TORRE I. Corsi S.E. Focardi A. Arukwe 2008 http://hdl.handle.net/2434/652588 https://doi.org/10.1080/02757540801966272 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000257093900001 Scientific Meeting of the 5th Congress of the National-Inter-University-Consortium-for-Marine-Sciences volume:24 issue:suppl. 1 firstpage:1 lastpage:7 numberofpages:7 journal:CHEMISTRY IN ECOLOGY http://hdl.handle.net/2434/652588 doi:10.1080/02757540801966272 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-47249120194 2,4,6-trinitrotoluene estrogen receptor aromatase StAR P450scc European eel Settore BIO/07 - Ecologia info:eu-repo/semantics/article 2008 ftunivmilanoair https://doi.org/10.1080/02757540801966272 2024-01-16T23:30:36Z The aim of the present study is to investigate the endocrine disrupting potential of the explosive 2,4,6-trinitrotoluene (TNT) on marine organisms using the European eel (Anguilla anguilla Linnaeus, 1758) as model fish species. Crucial brain steroidogenic parameters such as the estrogen receptors (ERs), P450 aromatase (CYP19), steroidogenic acute regulatory (StAR) protein and P450-mediated cholesterol side-chain cleavage (P450scc) enzyme were selected as potential biomarkers for the assessment of TNT endocrine disrupting potential. Juvenile European eels were exposed for 6 and 24h to 0.5, 1 and 2.5mg/l nominal concentration of TNT dissolved in dimethyl sulfoxide (DMSO). ER alpha CYP19, StAR protein and P450scc genes expression were quantified using real-time PCR. Exposure to TNT altered the expression of ER gene at the lowest TNT concentration after 6h of exposure, while after 24h gene expression levels increased respect to controls only in eels at the maximum TNT dose (2.5mg/l). A significant increase of CYP19 gene expression occurred after 6 h in all eels exposed to TNT compared to controls. On the opposite at 24h, CYP19 expression seems reduced in eels exposed to 0.5 and 1mg TNT/l compared to 6h, while no changes were observed at the highest dose (2.5mg/l). Both StAR and P450scc gene expression were decreased at 6h in eels exposed to 0.5 and 1mg TNT/l, while at 2.5mg TNT/l, the gene expression levels were restored to background level. No further modulation was evident after 24h for both genes. The overall results indicate the ability of TNT to affect neurosteroidogenic pathways. Article in Journal/Newspaper Anguilla anguilla European eel The University of Milan: Archivio Istituzionale della Ricerca (AIR) Chemistry and Ecology 24 sup1 1 7 |
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The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
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ftunivmilanoair |
language |
English |
topic |
2,4,6-trinitrotoluene estrogen receptor aromatase StAR P450scc European eel Settore BIO/07 - Ecologia |
spellingShingle |
2,4,6-trinitrotoluene estrogen receptor aromatase StAR P450scc European eel Settore BIO/07 - Ecologia DELLA TORRE, CAMILLA CORSI, ILARIA FOCARDI, SILVANO ETTORE ARUKWE A. Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
topic_facet |
2,4,6-trinitrotoluene estrogen receptor aromatase StAR P450scc European eel Settore BIO/07 - Ecologia |
description |
The aim of the present study is to investigate the endocrine disrupting potential of the explosive 2,4,6-trinitrotoluene (TNT) on marine organisms using the European eel (Anguilla anguilla Linnaeus, 1758) as model fish species. Crucial brain steroidogenic parameters such as the estrogen receptors (ERs), P450 aromatase (CYP19), steroidogenic acute regulatory (StAR) protein and P450-mediated cholesterol side-chain cleavage (P450scc) enzyme were selected as potential biomarkers for the assessment of TNT endocrine disrupting potential. Juvenile European eels were exposed for 6 and 24h to 0.5, 1 and 2.5mg/l nominal concentration of TNT dissolved in dimethyl sulfoxide (DMSO). ER alpha CYP19, StAR protein and P450scc genes expression were quantified using real-time PCR. Exposure to TNT altered the expression of ER gene at the lowest TNT concentration after 6h of exposure, while after 24h gene expression levels increased respect to controls only in eels at the maximum TNT dose (2.5mg/l). A significant increase of CYP19 gene expression occurred after 6 h in all eels exposed to TNT compared to controls. On the opposite at 24h, CYP19 expression seems reduced in eels exposed to 0.5 and 1mg TNT/l compared to 6h, while no changes were observed at the highest dose (2.5mg/l). Both StAR and P450scc gene expression were decreased at 6h in eels exposed to 0.5 and 1mg TNT/l, while at 2.5mg TNT/l, the gene expression levels were restored to background level. No further modulation was evident after 24h for both genes. The overall results indicate the ability of TNT to affect neurosteroidogenic pathways. |
author2 |
C. DELLA TORRE I. Corsi S.E. Focardi A. Arukwe |
format |
Article in Journal/Newspaper |
author |
DELLA TORRE, CAMILLA CORSI, ILARIA FOCARDI, SILVANO ETTORE ARUKWE A. |
author_facet |
DELLA TORRE, CAMILLA CORSI, ILARIA FOCARDI, SILVANO ETTORE ARUKWE A. |
author_sort |
DELLA TORRE, CAMILLA |
title |
Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
title_short |
Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
title_full |
Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
title_fullStr |
Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
title_full_unstemmed |
Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidogenesis in the European eel (Anguilla anguilla; Linnaeus 1758) |
title_sort |
effects of 2,4,6-trinitrotoluene (tnt) on neurosteroidogenesis in the european eel (anguilla anguilla; linnaeus 1758) |
publishDate |
2008 |
url |
http://hdl.handle.net/2434/652588 https://doi.org/10.1080/02757540801966272 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000257093900001 Scientific Meeting of the 5th Congress of the National-Inter-University-Consortium-for-Marine-Sciences volume:24 issue:suppl. 1 firstpage:1 lastpage:7 numberofpages:7 journal:CHEMISTRY IN ECOLOGY http://hdl.handle.net/2434/652588 doi:10.1080/02757540801966272 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-47249120194 |
op_doi |
https://doi.org/10.1080/02757540801966272 |
container_title |
Chemistry and Ecology |
container_volume |
24 |
container_issue |
sup1 |
container_start_page |
1 |
op_container_end_page |
7 |
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1790596788761133056 |