Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system
The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus pop...
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Online Access: | http://hdl.handle.net/2434/636318 https://doi.org/10.1016/j.scitotenv.2019.04.005 |
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ftunivmilanoair:oai:air.unimi.it:2434/636318 2024-02-04T10:03:24+01:00 Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system O. Migliaccio A. Pinsino E. Maffioli A. M. Smith C. Agnisola V. Matranga S. Nonnis G. Tedeschi M. Byrne M. C. Gambi A. Palumbo O. Migliaccio A. Pinsino E. Maffioli A.M. Smith C. Agnisola V. Matranga S. Nonni G. Tedeschi M. Byrne M.C. Gambi A. Palumbo 2019-07-01 http://hdl.handle.net/2434/636318 https://doi.org/10.1016/j.scitotenv.2019.04.005 eng eng Elsevier info:eu-repo/semantics/altIdentifier/pmid/30981169 info:eu-repo/semantics/altIdentifier/wos/WOS:000466979400087 volume:672 firstpage:938 lastpage:950 numberofpages:13 journal:SCIENCE OF THE TOTAL ENVIRONMENT http://hdl.handle.net/2434/636318 doi:10.1016/j.scitotenv.2019.04.005 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064086391 info:eu-repo/semantics/openAccess Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchins Settore BIO/10 - Biochimica info:eu-repo/semantics/article 2019 ftunivmilanoair https://doi.org/10.1016/j.scitotenv.2019.04.005 2024-01-09T23:45:14Z The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus population resident in low-pH (7.8 ± 0.2) compared to that at two control sites (pH 8.02 ± 0.00; 8.02 ± 0.01). The novelty of the present study is the analysis of the sea urchin immune cells, the sentinels of environmental stress responses, by a wide-ranging approach, including cell morphology, biochemistry and proteomics. Immune cell proteomics showed that 311 proteins were differentially expressed in urchins across sites with a general shift towards antioxidant processes in the vent urchins. The vent urchin immune cells showed higher levels of total antioxidant capacity, up-regulation of phagosome and microsomal proteins, enzymes of ammonium metabolism, amino-acid degradation, and modulation of carbon metabolism proteins. Lipid-hydroperoxides and nitric oxide levels were not different in urchins from the different sites. No differences in the coelomic fluid pH, immune cell composition, animal respiration, nitrogen excretion and skeletal mineralogy were observed. Our results reveal the phenotypic plasticity of the immune system of sea urchins adapted to life at vent site, under conditions commensurate with near-future ocean acidification projections. Article in Journal/Newspaper Ocean acidification The University of Milan: Archivio Istituzionale della Ricerca (AIR) The Sentinels ENVELOPE(-36.267,-36.267,-54.267,-54.267) Science of The Total Environment 672 938 950 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchins Settore BIO/10 - Biochimica |
spellingShingle |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchins Settore BIO/10 - Biochimica O. Migliaccio A. Pinsino E. Maffioli A. M. Smith C. Agnisola V. Matranga S. Nonnis G. Tedeschi M. Byrne M. C. Gambi A. Palumbo Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
topic_facet |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchins Settore BIO/10 - Biochimica |
description |
The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus population resident in low-pH (7.8 ± 0.2) compared to that at two control sites (pH 8.02 ± 0.00; 8.02 ± 0.01). The novelty of the present study is the analysis of the sea urchin immune cells, the sentinels of environmental stress responses, by a wide-ranging approach, including cell morphology, biochemistry and proteomics. Immune cell proteomics showed that 311 proteins were differentially expressed in urchins across sites with a general shift towards antioxidant processes in the vent urchins. The vent urchin immune cells showed higher levels of total antioxidant capacity, up-regulation of phagosome and microsomal proteins, enzymes of ammonium metabolism, amino-acid degradation, and modulation of carbon metabolism proteins. Lipid-hydroperoxides and nitric oxide levels were not different in urchins from the different sites. No differences in the coelomic fluid pH, immune cell composition, animal respiration, nitrogen excretion and skeletal mineralogy were observed. Our results reveal the phenotypic plasticity of the immune system of sea urchins adapted to life at vent site, under conditions commensurate with near-future ocean acidification projections. |
author2 |
O. Migliaccio A. Pinsino E. Maffioli A.M. Smith C. Agnisola V. Matranga S. Nonni G. Tedeschi M. Byrne M.C. Gambi A. Palumbo |
format |
Article in Journal/Newspaper |
author |
O. Migliaccio A. Pinsino E. Maffioli A. M. Smith C. Agnisola V. Matranga S. Nonnis G. Tedeschi M. Byrne M. C. Gambi A. Palumbo |
author_facet |
O. Migliaccio A. Pinsino E. Maffioli A. M. Smith C. Agnisola V. Matranga S. Nonnis G. Tedeschi M. Byrne M. C. Gambi A. Palumbo |
author_sort |
O. Migliaccio |
title |
Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
title_short |
Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
title_full |
Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
title_fullStr |
Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
title_full_unstemmed |
Living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a CO2 vent system |
title_sort |
living in future ocean acidification, physiological adaptive responses of the immune systems of sea urchins resident at a co2 vent system |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://hdl.handle.net/2434/636318 https://doi.org/10.1016/j.scitotenv.2019.04.005 |
long_lat |
ENVELOPE(-36.267,-36.267,-54.267,-54.267) |
geographic |
The Sentinels |
geographic_facet |
The Sentinels |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/30981169 info:eu-repo/semantics/altIdentifier/wos/WOS:000466979400087 volume:672 firstpage:938 lastpage:950 numberofpages:13 journal:SCIENCE OF THE TOTAL ENVIRONMENT http://hdl.handle.net/2434/636318 doi:10.1016/j.scitotenv.2019.04.005 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064086391 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2019.04.005 |
container_title |
Science of The Total Environment |
container_volume |
672 |
container_start_page |
938 |
op_container_end_page |
950 |
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1789970789657739264 |