Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica)
A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, result...
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ftunivmilanoair:oai:air.unimi.it:2434/621441 2024-01-21T10:01:27+01:00 Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) MICHAUD L DI CELLO F BRILLI M FANI R LO GIUDICE A BRUNI V L. Michaud F. DI CELLO M. Brilli R. Fani A. LO GIUDICE V. Bruni 2004 http://hdl.handle.net/2434/621441 https://doi.org/10.1016/S0378-1097(03)00857-7 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000188615500010 volume:230 issue:1 firstpage:63 lastpage:71 numberofpages:9 journal:FEMS MICROBIOLOGY LETTERS http://hdl.handle.net/2434/621441 doi:10.1016/S0378-1097(03)00857-7 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0347753489 info:eu-repo/semantics/openAccess Antarctic marine bacteria biodiversity 16S rDNA amplified rDNA restriction analysi random amplified polymorphic DNA analysis Settore BIO/19 - Microbiologia Generale info:eu-repo/semantics/article 2004 ftunivmilanoair https://doi.org/10.1016/S0378-1097(03)00857-7 2023-12-26T23:33:24Z A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out. Article in Journal/Newspaper Antarc* Antarctic Antarctica Ross Sea The University of Milan: Archivio Istituzionale della Ricerca (AIR) Antarctic Ross Sea Terra Nova Bay FEMS Microbiology Letters 230 1 63 71 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Antarctic marine bacteria biodiversity 16S rDNA amplified rDNA restriction analysi random amplified polymorphic DNA analysis Settore BIO/19 - Microbiologia Generale |
spellingShingle |
Antarctic marine bacteria biodiversity 16S rDNA amplified rDNA restriction analysi random amplified polymorphic DNA analysis Settore BIO/19 - Microbiologia Generale MICHAUD L DI CELLO F BRILLI M FANI R LO GIUDICE A BRUNI V Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
topic_facet |
Antarctic marine bacteria biodiversity 16S rDNA amplified rDNA restriction analysi random amplified polymorphic DNA analysis Settore BIO/19 - Microbiologia Generale |
description |
A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out. |
author2 |
L. Michaud F. DI CELLO M. Brilli R. Fani A. LO GIUDICE V. Bruni |
format |
Article in Journal/Newspaper |
author |
MICHAUD L DI CELLO F BRILLI M FANI R LO GIUDICE A BRUNI V |
author_facet |
MICHAUD L DI CELLO F BRILLI M FANI R LO GIUDICE A BRUNI V |
author_sort |
MICHAUD L |
title |
Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
title_short |
Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
title_full |
Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
title_fullStr |
Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
title_full_unstemmed |
Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica) |
title_sort |
biodiversity of cultivable psychrotrophic marine bacteria isolated from terra nova bay (ross sea, antarctica) |
publishDate |
2004 |
url |
http://hdl.handle.net/2434/621441 https://doi.org/10.1016/S0378-1097(03)00857-7 |
geographic |
Antarctic Ross Sea Terra Nova Bay |
geographic_facet |
Antarctic Ross Sea Terra Nova Bay |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000188615500010 volume:230 issue:1 firstpage:63 lastpage:71 numberofpages:9 journal:FEMS MICROBIOLOGY LETTERS http://hdl.handle.net/2434/621441 doi:10.1016/S0378-1097(03)00857-7 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0347753489 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/S0378-1097(03)00857-7 |
container_title |
FEMS Microbiology Letters |
container_volume |
230 |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
71 |
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1788691376162996224 |