Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica)

A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, result...

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Bibliographic Details
Published in:FEMS Microbiology Letters
Main Authors: MICHAUD L, DI CELLO F, BRILLI M, FANI R, LO GIUDICE A, BRUNI V
Other Authors: L. Michaud, F. DI CELLO, M. Brilli, R. Fani, A. LO GIUDICE, V. Bruni
Format: Article in Journal/Newspaper
Language:English
Published: 2004
Subjects:
Antarctic marine bacteria
biodiversity
16S rDNA
amplified rDNA restriction analysi
random amplified polymorphic DNA analysis
Settore BIO/19 - Microbiologia Generale
Antarctic
Ross Sea
Terra Nova Bay
Antarc*
Antarctica
Online Access:http://hdl.handle.net/2434/621441
https://doi.org/10.1016/S0378-1097(03)00857-7
Description
Summary:A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out.

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