Molecular analysis and mapping of gst genes
The glutathione(GSH)-glutathione-S-transferase(GST) system is a detoxification system capable of inactivating many toxic molecules. It is present in a wide variety of organisms, from mammals to insects and plants, in which it is involved in conferring tolerance to different classes of herbicides, in...
Main Authors: | , , , |
---|---|
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1995
|
Subjects: | |
Online Access: | http://hdl.handle.net/2434/427181 http://www.agron.missouri.edu/mnl/69/122rossini.html |
id |
ftunivmilanoair:oai:air.unimi.it:2434/427181 |
---|---|
record_format |
openpolar |
spelling |
ftunivmilanoair:oai:air.unimi.it:2434/427181 2024-04-21T08:03:45+00:00 Molecular analysis and mapping of gst genes L. Rossini C. Frova M.E. Pè GORLA, MIRELLA L. Rossini C. Frova P. M. E. M. Gorla 1995 http://hdl.handle.net/2434/427181 http://www.agron.missouri.edu/mnl/69/122rossini.html eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:A1995RZ16000004 volume:69 firstpage:101 lastpage:101 numberofpages:1 journal:MAIZE GENETICS COOPERATION NEWSLETTER http://hdl.handle.net/2434/427181 http://www.agron.missouri.edu/mnl/69/122rossini.html Settore AGR/07 - Genetica Agraria info:eu-repo/semantics/article 1995 ftunivmilanoair 2024-03-27T16:40:11Z The glutathione(GSH)-glutathione-S-transferase(GST) system is a detoxification system capable of inactivating many toxic molecules. It is present in a wide variety of organisms, from mammals to insects and plants, in which it is involved in conferring tolerance to different classes of herbicides, including s-triazines, acetanilides and thiocarbamates. In maize, three gst genes (gst1, gstII, gstIII) (Shah et al., Plant Mol Biol 6:203-211, 1986; Moore et al. Nucleic Acids Res. 14:7277-7235,1986; A. Greenland, personal communication) have been identified and sequenced. [Ed. note: Temporary symbols pending clarifications of gene relationships.] They belong to a gene family and show extensive sequence homology at both DNA and protein levels. They encode for four isozymes which are active as dimers: GSTI, GSTIII and GSTIV are homodimers of 29, 26 and 27 kDa subunits respectively, while GSTII is a heterodimer of 29 (same as GSTI) and 27 (same as GSTIV) kDa subunits. We found evidence that the number of maize gst genes and isozymes is higher, some isoforms being tissue-specific while others are expressed in most tissues. In particular, the two major bands detectable in electrophoretic enzyme assay (native PAGE) are expressed in roots, leaves and scutella. By screening numerous maize genotypes we have identified two inbred lines, B37 and B83, lacking these two bands in all tissues. Analysis of null/+ and null/null F1s indicated that the null mutations are recessive and allelic in the two inbreds. In order to identify to which of the characterized GST isoforms these bands correspond, we have carried out Northern experiments to analyze the transcription pattern of gstI and gstII in roots of normal and of the two null lines. As specific probes we have used a cDNA clone of gstII (kindly provided by A. Greenland) and a probe including the first exon of gstI synthesized via PCR on the basis of the published sequence information. gstII transcript was detected in all genotypes, while gstI mRNA was absent in the null lines. These ... Article in Journal/Newspaper Greenland The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Settore AGR/07 - Genetica Agraria |
spellingShingle |
Settore AGR/07 - Genetica Agraria L. Rossini C. Frova M.E. Pè GORLA, MIRELLA Molecular analysis and mapping of gst genes |
topic_facet |
Settore AGR/07 - Genetica Agraria |
description |
The glutathione(GSH)-glutathione-S-transferase(GST) system is a detoxification system capable of inactivating many toxic molecules. It is present in a wide variety of organisms, from mammals to insects and plants, in which it is involved in conferring tolerance to different classes of herbicides, including s-triazines, acetanilides and thiocarbamates. In maize, three gst genes (gst1, gstII, gstIII) (Shah et al., Plant Mol Biol 6:203-211, 1986; Moore et al. Nucleic Acids Res. 14:7277-7235,1986; A. Greenland, personal communication) have been identified and sequenced. [Ed. note: Temporary symbols pending clarifications of gene relationships.] They belong to a gene family and show extensive sequence homology at both DNA and protein levels. They encode for four isozymes which are active as dimers: GSTI, GSTIII and GSTIV are homodimers of 29, 26 and 27 kDa subunits respectively, while GSTII is a heterodimer of 29 (same as GSTI) and 27 (same as GSTIV) kDa subunits. We found evidence that the number of maize gst genes and isozymes is higher, some isoforms being tissue-specific while others are expressed in most tissues. In particular, the two major bands detectable in electrophoretic enzyme assay (native PAGE) are expressed in roots, leaves and scutella. By screening numerous maize genotypes we have identified two inbred lines, B37 and B83, lacking these two bands in all tissues. Analysis of null/+ and null/null F1s indicated that the null mutations are recessive and allelic in the two inbreds. In order to identify to which of the characterized GST isoforms these bands correspond, we have carried out Northern experiments to analyze the transcription pattern of gstI and gstII in roots of normal and of the two null lines. As specific probes we have used a cDNA clone of gstII (kindly provided by A. Greenland) and a probe including the first exon of gstI synthesized via PCR on the basis of the published sequence information. gstII transcript was detected in all genotypes, while gstI mRNA was absent in the null lines. These ... |
author2 |
L. Rossini C. Frova P. M. E. M. Gorla |
format |
Article in Journal/Newspaper |
author |
L. Rossini C. Frova M.E. Pè GORLA, MIRELLA |
author_facet |
L. Rossini C. Frova M.E. Pè GORLA, MIRELLA |
author_sort |
L. Rossini |
title |
Molecular analysis and mapping of gst genes |
title_short |
Molecular analysis and mapping of gst genes |
title_full |
Molecular analysis and mapping of gst genes |
title_fullStr |
Molecular analysis and mapping of gst genes |
title_full_unstemmed |
Molecular analysis and mapping of gst genes |
title_sort |
molecular analysis and mapping of gst genes |
publishDate |
1995 |
url |
http://hdl.handle.net/2434/427181 http://www.agron.missouri.edu/mnl/69/122rossini.html |
genre |
Greenland |
genre_facet |
Greenland |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:A1995RZ16000004 volume:69 firstpage:101 lastpage:101 numberofpages:1 journal:MAIZE GENETICS COOPERATION NEWSLETTER http://hdl.handle.net/2434/427181 http://www.agron.missouri.edu/mnl/69/122rossini.html |
_version_ |
1796943478530244608 |