Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones
The arctic-alpine White Mountain Orchid (Pseudorchis albida) has so-far resisted attempts at in vitro reproduction from seed, but a practical method is required for its conservation. We hypothesised that, having similar seeds to the notoriously intractable orchid Cypripedium calceolus, immature seed...
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ftunivmilanoair:oai:air.unimi.it:2434/223833 2024-04-14T08:08:04+00:00 Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones S. Pierce B. E. L. Cerabolini S. Pierce B.E.L. Cerabolini 2011 http://hdl.handle.net/2434/223833 https://doi.org/10.15258/sst.2011.39.1.17 eng eng International Seed Testing Association info:eu-repo/semantics/altIdentifier/wos/WOS:000289929500017 volume:39 issue:1 firstpage:199 lastpage:203 numberofpages:5 journal:SEED SCIENCE AND TECHNOLOGY http://hdl.handle.net/2434/223833 doi:10.15258/sst.2011.39.1.17 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79955124148 info:eu-repo/semantics/closedAccess Settore BIO/03 - Botanica Ambientale e Applicata info:eu-repo/semantics/article 2011 ftunivmilanoair https://doi.org/10.15258/sst.2011.39.1.17 2024-03-27T16:34:25Z The arctic-alpine White Mountain Orchid (Pseudorchis albida) has so-far resisted attempts at in vitro reproduction from seed, but a practical method is required for its conservation. We hypothesised that, having similar seeds to the notoriously intractable orchid Cypripedium calceolus, immature seeds can germinate in vitro. We collected mature and immature seeds from the Italian Alps, and sowed these in sterile conditions on Malmgren's basal nutrient medium modified with either phytohormones or complex organic media (COM). Different COM treatments included coconut milk, banana powder, malt extract and yeast extract, all at a concentration of 5%. Phytohormones, all at 20 mg L -1, included 6-benzylaminopurine (BA), gibberellic acid (GA 3), kinetin and thidiazuron (TDZ), added to autoclaved growth media via filter sterilization. After six months we found absolutely no germination of mature seeds for any treatment. Only 3.9% of immature seeds germinated in the control treatment and germination in all COM treatments was not significantly greater than this. However, most phytohormones significantly increased germination of immature seed and promoted protocorm development, BA being most effective (50.5% germination) - this method can therefore be used to propagate the species. Article in Journal/Newspaper Arctic The University of Milan: Archivio Istituzionale della Ricerca (AIR) Arctic Seed Science and Technology 39 1 199 203 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Settore BIO/03 - Botanica Ambientale e Applicata |
spellingShingle |
Settore BIO/03 - Botanica Ambientale e Applicata S. Pierce B. E. L. Cerabolini Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
topic_facet |
Settore BIO/03 - Botanica Ambientale e Applicata |
description |
The arctic-alpine White Mountain Orchid (Pseudorchis albida) has so-far resisted attempts at in vitro reproduction from seed, but a practical method is required for its conservation. We hypothesised that, having similar seeds to the notoriously intractable orchid Cypripedium calceolus, immature seeds can germinate in vitro. We collected mature and immature seeds from the Italian Alps, and sowed these in sterile conditions on Malmgren's basal nutrient medium modified with either phytohormones or complex organic media (COM). Different COM treatments included coconut milk, banana powder, malt extract and yeast extract, all at a concentration of 5%. Phytohormones, all at 20 mg L -1, included 6-benzylaminopurine (BA), gibberellic acid (GA 3), kinetin and thidiazuron (TDZ), added to autoclaved growth media via filter sterilization. After six months we found absolutely no germination of mature seeds for any treatment. Only 3.9% of immature seeds germinated in the control treatment and germination in all COM treatments was not significantly greater than this. However, most phytohormones significantly increased germination of immature seed and promoted protocorm development, BA being most effective (50.5% germination) - this method can therefore be used to propagate the species. |
author2 |
S. Pierce B.E.L. Cerabolini |
format |
Article in Journal/Newspaper |
author |
S. Pierce B. E. L. Cerabolini |
author_facet |
S. Pierce B. E. L. Cerabolini |
author_sort |
S. Pierce |
title |
Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
title_short |
Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
title_full |
Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
title_fullStr |
Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
title_full_unstemmed |
Asymbiotic germination of the White Mountain Orchid (Pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
title_sort |
asymbiotic germination of the white mountain orchid (pseudorchis albida) from immature seed on media enriched with complex organics or phytohormones |
publisher |
International Seed Testing Association |
publishDate |
2011 |
url |
http://hdl.handle.net/2434/223833 https://doi.org/10.15258/sst.2011.39.1.17 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000289929500017 volume:39 issue:1 firstpage:199 lastpage:203 numberofpages:5 journal:SEED SCIENCE AND TECHNOLOGY http://hdl.handle.net/2434/223833 doi:10.15258/sst.2011.39.1.17 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-79955124148 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.15258/sst.2011.39.1.17 |
container_title |
Seed Science and Technology |
container_volume |
39 |
container_issue |
1 |
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199 |
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203 |
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