Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin
An investigation was made into the occurrence and biodiversity of Geodermatophilaceae on 78 samples of altered stone surfaces from 24 monuments and natural stones in the Mediterranean basin; it was found that the total microbial counts ranged between 0 and 10(7) cfu g(-1) dry weight. Members of the...
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Online Access: | http://hdl.handle.net/2434/192870 https://doi.org/10.1046/j.1462-2920.2001.00217.x |
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ftunivmilanoair:oai:air.unimi.it:2434/192870 2024-02-11T09:57:06+01:00 Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin C. Urzi L. Brusetti P. Salamone E. Stackebrandt C. Sorlini D. Daffonchio C. Urzi L. Brusetti P. Salamone C. Sorlini E. Stackebrandt D. Daffonchio 2001-07 http://hdl.handle.net/2434/192870 https://doi.org/10.1046/j.1462-2920.2001.00217.x eng eng BLACKWELL SCIENCE LTD info:eu-repo/semantics/altIdentifier/pmid/11553237 info:eu-repo/semantics/altIdentifier/wos/WOS:000171022900006 volume:3 issue:7 firstpage:471 lastpage:479 10.1046/j.1462-2920.2001.00217.x journal:ENVIRONMENTAL MICROBIOLOGY http://hdl.handle.net/2434/192870 doi:10.1046/j.1462-2920.2001.00217.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0035210325 BACTERIA IDENTIFICATION MICROORGANISMS ACTINOMYCETE PROPOSAL STRAIN MARBLE ROCKS SOILS FUNGI Settore AGR/16 - Microbiologia Agraria info:eu-repo/semantics/article 2001 ftunivmilanoair https://doi.org/10.1046/j.1462-2920.2001.00217.x 2024-01-23T23:23:08Z An investigation was made into the occurrence and biodiversity of Geodermatophilaceae on 78 samples of altered stone surfaces from 24 monuments and natural stones in the Mediterranean basin; it was found that the total microbial counts ranged between 0 and 10(7) cfu g(-1) dry weight. Members of the Geodermatophilaceae family were isolated from 22 of the 78 samples examined, with the incidence of Geodermatophilaceae colonies in the cultivable population ranging from 1% to 100%. The highest percentage was found in six samples of markedly deteriorated stone. Sixty-five strains randomly isolated from the plates were clustered in six different groups by amplified 16S rDNA restriction analysis (ARDRA) using five different restriction enzymes. Twenty-five strains, representing all the ARDRA haplotypes, were characterized further by partial sequencing (350-550 bp) of the 16S rDNA and by analysing 76 morphological, metabolic and physiological properties. The strains were associated with three well-separated clusters of the genera Geodermatophilus, Blastococcus and Modestobacter. On the basis of 16S rDNA sequence and ARDRA analysis, only two strains were found to be related to the two reference strains of Geodermatophilus. All the others could be grouped with Blastococcus aggregatus (19 strains) or the Antarctic species Modestobacter multiseptatus (44 strains), suggesting that it is these two groups, rather than Geodermatophilus, that tend to colonize the stone surfaces, and that Modestobacter-like strains are also found in temperate/Mediterranean climates. From the BOX-polymerase chain reaction (PCR) data, it can be seen that the Modestobacter-like strains, belonging to the most represented ARDRA haplotype (haplotype B, 34 strains), are very polymorphic and that, over a stone surface, there is a wide genetic diversity at the microsite level Article in Journal/Newspaper Antarc* Antarctic The University of Milan: Archivio Istituzionale della Ricerca (AIR) Antarctic The Antarctic Environmental Microbiology 3 7 471 479 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
BACTERIA IDENTIFICATION MICROORGANISMS ACTINOMYCETE PROPOSAL STRAIN MARBLE ROCKS SOILS FUNGI Settore AGR/16 - Microbiologia Agraria |
spellingShingle |
BACTERIA IDENTIFICATION MICROORGANISMS ACTINOMYCETE PROPOSAL STRAIN MARBLE ROCKS SOILS FUNGI Settore AGR/16 - Microbiologia Agraria C. Urzi L. Brusetti P. Salamone E. Stackebrandt C. Sorlini D. Daffonchio Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
topic_facet |
BACTERIA IDENTIFICATION MICROORGANISMS ACTINOMYCETE PROPOSAL STRAIN MARBLE ROCKS SOILS FUNGI Settore AGR/16 - Microbiologia Agraria |
description |
An investigation was made into the occurrence and biodiversity of Geodermatophilaceae on 78 samples of altered stone surfaces from 24 monuments and natural stones in the Mediterranean basin; it was found that the total microbial counts ranged between 0 and 10(7) cfu g(-1) dry weight. Members of the Geodermatophilaceae family were isolated from 22 of the 78 samples examined, with the incidence of Geodermatophilaceae colonies in the cultivable population ranging from 1% to 100%. The highest percentage was found in six samples of markedly deteriorated stone. Sixty-five strains randomly isolated from the plates were clustered in six different groups by amplified 16S rDNA restriction analysis (ARDRA) using five different restriction enzymes. Twenty-five strains, representing all the ARDRA haplotypes, were characterized further by partial sequencing (350-550 bp) of the 16S rDNA and by analysing 76 morphological, metabolic and physiological properties. The strains were associated with three well-separated clusters of the genera Geodermatophilus, Blastococcus and Modestobacter. On the basis of 16S rDNA sequence and ARDRA analysis, only two strains were found to be related to the two reference strains of Geodermatophilus. All the others could be grouped with Blastococcus aggregatus (19 strains) or the Antarctic species Modestobacter multiseptatus (44 strains), suggesting that it is these two groups, rather than Geodermatophilus, that tend to colonize the stone surfaces, and that Modestobacter-like strains are also found in temperate/Mediterranean climates. From the BOX-polymerase chain reaction (PCR) data, it can be seen that the Modestobacter-like strains, belonging to the most represented ARDRA haplotype (haplotype B, 34 strains), are very polymorphic and that, over a stone surface, there is a wide genetic diversity at the microsite level |
author2 |
C. Urzi L. Brusetti P. Salamone C. Sorlini E. Stackebrandt D. Daffonchio |
format |
Article in Journal/Newspaper |
author |
C. Urzi L. Brusetti P. Salamone E. Stackebrandt C. Sorlini D. Daffonchio |
author_facet |
C. Urzi L. Brusetti P. Salamone E. Stackebrandt C. Sorlini D. Daffonchio |
author_sort |
C. Urzi |
title |
Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
title_short |
Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
title_full |
Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
title_fullStr |
Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
title_full_unstemmed |
Biodiversity of Geodermatophilaceae isolated from altered stones and monuments in the Mediterranean basin |
title_sort |
biodiversity of geodermatophilaceae isolated from altered stones and monuments in the mediterranean basin |
publisher |
BLACKWELL SCIENCE LTD |
publishDate |
2001 |
url |
http://hdl.handle.net/2434/192870 https://doi.org/10.1046/j.1462-2920.2001.00217.x |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/11553237 info:eu-repo/semantics/altIdentifier/wos/WOS:000171022900006 volume:3 issue:7 firstpage:471 lastpage:479 10.1046/j.1462-2920.2001.00217.x journal:ENVIRONMENTAL MICROBIOLOGY http://hdl.handle.net/2434/192870 doi:10.1046/j.1462-2920.2001.00217.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0035210325 |
op_doi |
https://doi.org/10.1046/j.1462-2920.2001.00217.x |
container_title |
Environmental Microbiology |
container_volume |
3 |
container_issue |
7 |
container_start_page |
471 |
op_container_end_page |
479 |
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1790608575485181952 |