Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere
The prevalence of blaTEM genes conferring ampicillin resistance (Ampr) in different soils was determined to clarify the environmental distribution of resistance determinants of major clinical importance. Samples were collected from 14 sites in New Zealand, mainland Norway, Svalbard, and 2 soil micro...
Published in: | Microbial Ecology in Health and Disease |
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2008
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Online Access: | http://hdl.handle.net/2434/141475 https://doi.org/10.1080/08910600701838244 |
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ftunivmilanoair:oai:air.unimi.it:2434/141475 2024-02-11T10:01:01+01:00 Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere L. Brusetti S. Borin A. Rizzi D.G. Daffonchio T. Glad P. Myren P. J. Johnsen P. Carter K. M. Nielsen L. Brusetti T. Glad S. Borin P. Myren A. Rizzi P.J. Johnsen P. Carter D.G. Daffonchio K.M. Nielsen 2008 http://hdl.handle.net/2434/141475 https://doi.org/10.1080/08910600701838244 eng eng Scandinavian University Press volume:20 issue:1 firstpage:27 lastpage:36 journal:MICROBIAL ECOLOGY IN HEALTH AND DISEASE http://hdl.handle.net/2434/141475 doi:10.1080/08910600701838244 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-42049095073 Ampicillin resistance blaTEM allele Geographic distribution Natural reservoir Non-selective environment Soil Settore AGR/16 - Microbiologia Agraria info:eu-repo/semantics/article 2008 ftunivmilanoair https://doi.org/10.1080/08910600701838244 2024-01-23T23:20:01Z The prevalence of blaTEM genes conferring ampicillin resistance (Ampr) in different soils was determined to clarify the environmental distribution of resistance determinants of major clinical importance. Samples were collected from 14 sites in New Zealand, mainland Norway, Svalbard, and 2 soil microcosms made of compost purchased in Italy. The Ampr bacteria represented 1.7–100% of the cultivable microflora with an average of 28%. Approximately 1200 Ampr isolates were further analyzed. Although >50% of the resistant isolates were capable of β-lactam-ring (nitrocefin) degradation, none carried a PCR-detectable blaTEM gene. The proportion of blaTEM genes in the culturable Ampr isolates was <0.07%. The overall blaTEM gene prevalence was determined by blaTEM-specific PCR of DNA extracted directly from the environmental sample. DNA hybridization was performed on selected samples with a detection limit of 11 blaTEM genes per PCR sample. Our analysis indicates that the prevalence of blaTEM carrying bacteria is <1 per 1000 to 100 000 bacteria in the samples analyzed. The study suggests that blaTEM genes are rare in soil environments, in contrast to their increasing prevalence in some clinical and commensal bacterial populations. The frequent observation of nitrocefin-degrading capacity among the sampled isolates suggests that other mechanisms conferring enzyme-mediated resistance to β-lactam antibiotics are widespread in Arctic and agricultural soil environments. Article in Journal/Newspaper Arctic Svalbard The University of Milan: Archivio Istituzionale della Ricerca (AIR) Arctic Svalbard Norway New Zealand Microbial Ecology in Health and Disease 20 1 27 36 |
institution |
Open Polar |
collection |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
op_collection_id |
ftunivmilanoair |
language |
English |
topic |
Ampicillin resistance blaTEM allele Geographic distribution Natural reservoir Non-selective environment Soil Settore AGR/16 - Microbiologia Agraria |
spellingShingle |
Ampicillin resistance blaTEM allele Geographic distribution Natural reservoir Non-selective environment Soil Settore AGR/16 - Microbiologia Agraria L. Brusetti S. Borin A. Rizzi D.G. Daffonchio T. Glad P. Myren P. J. Johnsen P. Carter K. M. Nielsen Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
topic_facet |
Ampicillin resistance blaTEM allele Geographic distribution Natural reservoir Non-selective environment Soil Settore AGR/16 - Microbiologia Agraria |
description |
The prevalence of blaTEM genes conferring ampicillin resistance (Ampr) in different soils was determined to clarify the environmental distribution of resistance determinants of major clinical importance. Samples were collected from 14 sites in New Zealand, mainland Norway, Svalbard, and 2 soil microcosms made of compost purchased in Italy. The Ampr bacteria represented 1.7–100% of the cultivable microflora with an average of 28%. Approximately 1200 Ampr isolates were further analyzed. Although >50% of the resistant isolates were capable of β-lactam-ring (nitrocefin) degradation, none carried a PCR-detectable blaTEM gene. The proportion of blaTEM genes in the culturable Ampr isolates was <0.07%. The overall blaTEM gene prevalence was determined by blaTEM-specific PCR of DNA extracted directly from the environmental sample. DNA hybridization was performed on selected samples with a detection limit of 11 blaTEM genes per PCR sample. Our analysis indicates that the prevalence of blaTEM carrying bacteria is <1 per 1000 to 100 000 bacteria in the samples analyzed. The study suggests that blaTEM genes are rare in soil environments, in contrast to their increasing prevalence in some clinical and commensal bacterial populations. The frequent observation of nitrocefin-degrading capacity among the sampled isolates suggests that other mechanisms conferring enzyme-mediated resistance to β-lactam antibiotics are widespread in Arctic and agricultural soil environments. |
author2 |
L. Brusetti T. Glad S. Borin P. Myren A. Rizzi P.J. Johnsen P. Carter D.G. Daffonchio K.M. Nielsen |
format |
Article in Journal/Newspaper |
author |
L. Brusetti S. Borin A. Rizzi D.G. Daffonchio T. Glad P. Myren P. J. Johnsen P. Carter K. M. Nielsen |
author_facet |
L. Brusetti S. Borin A. Rizzi D.G. Daffonchio T. Glad P. Myren P. J. Johnsen P. Carter K. M. Nielsen |
author_sort |
L. Brusetti |
title |
Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
title_short |
Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
title_full |
Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
title_fullStr |
Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
title_full_unstemmed |
Low prevalence of blaTEM genes in Arctic environments and agricultural soil and rhizosphere |
title_sort |
low prevalence of blatem genes in arctic environments and agricultural soil and rhizosphere |
publisher |
Scandinavian University Press |
publishDate |
2008 |
url |
http://hdl.handle.net/2434/141475 https://doi.org/10.1080/08910600701838244 |
geographic |
Arctic Svalbard Norway New Zealand |
geographic_facet |
Arctic Svalbard Norway New Zealand |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_relation |
volume:20 issue:1 firstpage:27 lastpage:36 journal:MICROBIAL ECOLOGY IN HEALTH AND DISEASE http://hdl.handle.net/2434/141475 doi:10.1080/08910600701838244 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-42049095073 |
op_doi |
https://doi.org/10.1080/08910600701838244 |
container_title |
Microbial Ecology in Health and Disease |
container_volume |
20 |
container_issue |
1 |
container_start_page |
27 |
op_container_end_page |
36 |
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1790596746409148416 |