A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives
A 39-member library of bile acid derivatives was prepared starting from 3a,7a,12a-trihydroxy-5b-cholan-24-oic acid methyl ester using a combinatorial biocatalytic approach. A regioselective oxidation step, catalyzed by hydroxysteroid dehydrogenases, followed by an acylation step with a series of dif...
| Published in: | Biotechnology and Bioengineering |
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| Main Authors: | , , , , |
| Other Authors: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
John Wiley & Sons
2003
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| Subjects: | |
| Online Access: | http://hdl.handle.net/2434/10678 https://doi.org/10.1002/bit.10486 |
| _version_ | 1821764128463650816 |
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| author | F. Secundo G. Carrea S. Joppolo di Ventimiglia J. S. Dordick M. De Amici |
| author2 | F. Secundo G. Carrea M. De Amici S. Joppolo di Ventimiglia J.S. Dordick |
| author_facet | F. Secundo G. Carrea S. Joppolo di Ventimiglia J. S. Dordick M. De Amici |
| author_sort | F. Secundo |
| collection | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| container_issue | 4 |
| container_start_page | 391 |
| container_title | Biotechnology and Bioengineering |
| container_volume | 81 |
| description | A 39-member library of bile acid derivatives was prepared starting from 3a,7a,12a-trihydroxy-5b-cholan-24-oic acid methyl ester using a combinatorial biocatalytic approach. A regioselective oxidation step, catalyzed by hydroxysteroid dehydrogenases, followed by an acylation step with a series of different acyl donors catalyzed by Candida antarctica lipase B, led to the modification of the bile acid scaffold. Each member of the library was obtained in high purity and good yield. |
| format | Article in Journal/Newspaper |
| genre | Antarc* Antarctica |
| genre_facet | Antarc* Antarctica |
| id | ftunivmilanoair:oai:air.unimi.it:2434/10678 |
| institution | Open Polar |
| language | English |
| op_collection_id | ftunivmilanoair |
| op_container_end_page | 396 |
| op_doi | https://doi.org/10.1002/bit.10486 |
| op_relation | info:eu-repo/semantics/altIdentifier/pmid/12491524 info:eu-repo/semantics/altIdentifier/wos/WOS:000180570600002 volume:81 issue:4 firstpage:391 lastpage:396 journal:BIOTECHNOLOGY AND BIOENGINEERING http://hdl.handle.net/2434/10678 doi:10.1002/bit.10486 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037456178 |
| publishDate | 2003 |
| publisher | John Wiley & Sons |
| record_format | openpolar |
| spelling | ftunivmilanoair:oai:air.unimi.it:2434/10678 2025-01-16T19:32:06+00:00 A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives F. Secundo G. Carrea S. Joppolo di Ventimiglia J. S. Dordick M. De Amici F. Secundo G. Carrea M. De Amici S. Joppolo di Ventimiglia J.S. Dordick 2003-02-20 http://hdl.handle.net/2434/10678 https://doi.org/10.1002/bit.10486 eng eng John Wiley & Sons info:eu-repo/semantics/altIdentifier/pmid/12491524 info:eu-repo/semantics/altIdentifier/wos/WOS:000180570600002 volume:81 issue:4 firstpage:391 lastpage:396 journal:BIOTECHNOLOGY AND BIOENGINEERING http://hdl.handle.net/2434/10678 doi:10.1002/bit.10486 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0037456178 Bile acid Candida antarctica lipase B Combinatorial biocatalysi Hydroxysteroid dehydrogenases Settore CHIM/08 - Chimica Farmaceutica info:eu-repo/semantics/article 2003 ftunivmilanoair https://doi.org/10.1002/bit.10486 2024-01-09T23:15:15Z A 39-member library of bile acid derivatives was prepared starting from 3a,7a,12a-trihydroxy-5b-cholan-24-oic acid methyl ester using a combinatorial biocatalytic approach. A regioselective oxidation step, catalyzed by hydroxysteroid dehydrogenases, followed by an acylation step with a series of different acyl donors catalyzed by Candida antarctica lipase B, led to the modification of the bile acid scaffold. Each member of the library was obtained in high purity and good yield. Article in Journal/Newspaper Antarc* Antarctica The University of Milan: Archivio Istituzionale della Ricerca (AIR) Biotechnology and Bioengineering 81 4 391 396 |
| spellingShingle | Bile acid Candida antarctica lipase B Combinatorial biocatalysi Hydroxysteroid dehydrogenases Settore CHIM/08 - Chimica Farmaceutica F. Secundo G. Carrea S. Joppolo di Ventimiglia J. S. Dordick M. De Amici A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title | A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title_full | A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title_fullStr | A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title_full_unstemmed | A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title_short | A Combinatorial Biocatalysis Approach to an Array of Cholic Acid Derivatives |
| title_sort | combinatorial biocatalysis approach to an array of cholic acid derivatives |
| topic | Bile acid Candida antarctica lipase B Combinatorial biocatalysi Hydroxysteroid dehydrogenases Settore CHIM/08 - Chimica Farmaceutica |
| topic_facet | Bile acid Candida antarctica lipase B Combinatorial biocatalysi Hydroxysteroid dehydrogenases Settore CHIM/08 - Chimica Farmaceutica |
| url | http://hdl.handle.net/2434/10678 https://doi.org/10.1002/bit.10486 |