Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons
The basic understanding of both the physiology and ecology of psychrotolerant Antarctic bacteria is a crucial step for the optimization of their biodegradative activity in cold environments. The detection of cold-adapted hydrocarbon-degrading bacteria in Antarctic seawaters is certainly of great int...
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ftunivmessina:oai:cab.unime.it:355 2023-05-15T13:59:46+02:00 Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons Lo Giudice, Angelina Michaud, Luigi Gentile, Gabriella De Domenico, M. Bruni, Vivia 2005 application/pdf https://cab.unime.it/mus/355/ https://cab.unime.it/mus/355/1/c1a0501004.pdf http://antonello.unime.it/atti/ en eng https://cab.unime.it/mus/355/1/c1a0501004.pdf Lo Giudice, Angelina and Michaud, Luigi and Gentile, Gabriella and De Domenico, M. and Bruni, Vivia (2005) Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons. Atti della Accademia Peloritana dei Pericolanti, Classe di Scienze FF.MM.NN., LXXXIII. ISSN 1825 -1242 Classe di Scienze Fisiche Matematiche e Naturali Article PeerReviewed 2005 ftunivmessina 2022-03-14T13:24:24Z The basic understanding of both the physiology and ecology of psychrotolerant Antarctic bacteria is a crucial step for the optimization of their biodegradative activity in cold environments. The detection of cold-adapted hydrocarbon-degrading bacteria in Antarctic seawaters is certainly of great interest for bioremediative purpose in oil polluted marine Antarctic systems, where the introduction of non native species is not allowed. This study focused on psychrotolerant marine bacteria inhabiting an Antarctic coastal area directly influenced by the human activity at the Italian Research Station (Terra Nova Bay). Fifty bacterial strains were isolated from hydrocarbon-degrading enrichment cultures obtained from seawater samples collected in the inlet Road Bay (Ross Sea). A preliminary Restriction Fragment Length Polymorphism (RFLP) analysis, carried out on 16S rDNA amplified via PCR using RSAI and AluI restriction enzymes, was applied to cluster the isolates according to the restriction profile they showed. One representative isolate per cluster was selected for further characterization; to elucidate their taxonomic position, conventional phenotypic and phylogenetic analyses were performed. Results led to the identification of the isolates as members of ten genera belonging to four phylogenetic groups: the alfa- and gamma-proteobacteria subdivisions, the gram-positive branch and the Cytophaga-Flexibacter-Bacteroides (CFB) phylum. Results indicate a high degree of biodiversity within the peculiar ecophysiological group of the hydrocarbon-degrading marine bacteria. Article in Journal/Newspaper Antarc* Antarctic Ross Sea Università degli Studi di Messina: Messanae Universitas Studiorum Antarctic Road Bay ENVELOPE(164.122,164.122,-74.693,-74.693) Ross Sea Terra Nova Bay |
institution |
Open Polar |
collection |
Università degli Studi di Messina: Messanae Universitas Studiorum |
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ftunivmessina |
language |
English |
topic |
Classe di Scienze Fisiche Matematiche e Naturali |
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Classe di Scienze Fisiche Matematiche e Naturali Lo Giudice, Angelina Michaud, Luigi Gentile, Gabriella De Domenico, M. Bruni, Vivia Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
topic_facet |
Classe di Scienze Fisiche Matematiche e Naturali |
description |
The basic understanding of both the physiology and ecology of psychrotolerant Antarctic bacteria is a crucial step for the optimization of their biodegradative activity in cold environments. The detection of cold-adapted hydrocarbon-degrading bacteria in Antarctic seawaters is certainly of great interest for bioremediative purpose in oil polluted marine Antarctic systems, where the introduction of non native species is not allowed. This study focused on psychrotolerant marine bacteria inhabiting an Antarctic coastal area directly influenced by the human activity at the Italian Research Station (Terra Nova Bay). Fifty bacterial strains were isolated from hydrocarbon-degrading enrichment cultures obtained from seawater samples collected in the inlet Road Bay (Ross Sea). A preliminary Restriction Fragment Length Polymorphism (RFLP) analysis, carried out on 16S rDNA amplified via PCR using RSAI and AluI restriction enzymes, was applied to cluster the isolates according to the restriction profile they showed. One representative isolate per cluster was selected for further characterization; to elucidate their taxonomic position, conventional phenotypic and phylogenetic analyses were performed. Results led to the identification of the isolates as members of ten genera belonging to four phylogenetic groups: the alfa- and gamma-proteobacteria subdivisions, the gram-positive branch and the Cytophaga-Flexibacter-Bacteroides (CFB) phylum. Results indicate a high degree of biodiversity within the peculiar ecophysiological group of the hydrocarbon-degrading marine bacteria. |
format |
Article in Journal/Newspaper |
author |
Lo Giudice, Angelina Michaud, Luigi Gentile, Gabriella De Domenico, M. Bruni, Vivia |
author_facet |
Lo Giudice, Angelina Michaud, Luigi Gentile, Gabriella De Domenico, M. Bruni, Vivia |
author_sort |
Lo Giudice, Angelina |
title |
Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
title_short |
Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
title_full |
Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
title_fullStr |
Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
title_full_unstemmed |
Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
title_sort |
ecophysiological characterization of cultivable antarctic psychrotolerant marine bacteria able to degrade hydrocarbons |
publishDate |
2005 |
url |
https://cab.unime.it/mus/355/ https://cab.unime.it/mus/355/1/c1a0501004.pdf http://antonello.unime.it/atti/ |
long_lat |
ENVELOPE(164.122,164.122,-74.693,-74.693) |
geographic |
Antarctic Road Bay Ross Sea Terra Nova Bay |
geographic_facet |
Antarctic Road Bay Ross Sea Terra Nova Bay |
genre |
Antarc* Antarctic Ross Sea |
genre_facet |
Antarc* Antarctic Ross Sea |
op_relation |
https://cab.unime.it/mus/355/1/c1a0501004.pdf Lo Giudice, Angelina and Michaud, Luigi and Gentile, Gabriella and De Domenico, M. and Bruni, Vivia (2005) Ecophysiological characterization of cultivable Antarctic psychrotolerant marine bacteria able to degrade hydrocarbons. Atti della Accademia Peloritana dei Pericolanti, Classe di Scienze FF.MM.NN., LXXXIII. ISSN 1825 -1242 |
_version_ |
1766268528846962688 |