Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae
The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known...
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ftunivmaryland:oai:drum.lib.umd.edu:1903/27336 2023-05-15T14:01:11+02:00 Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae Ranjan, Peeyush Kateriya, Suneel 2018-01-24 application/pdf http://hdl.handle.net/1903/27336 https://doi.org/10.13016/xuor-xohf en_US eng Springer Nature Cell Biology & Molecular Genetics Digital Repository at the University of Maryland College of Computer, Mathematical & Natural Sciences University of Maryland (College Park, MD) https://doi.org/10.13016/xuor-xohf Ranjan, P., Kateriya, S. Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae. BMC Res Notes 11, 65 (2018). http://hdl.handle.net/1903/27336 Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Article 2018 ftunivmaryland https://doi.org/10.13016/xuor-xohf 2022-11-11T11:17:36Z The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants. https://doi.org/10.1186/s13104-018-3181-4 Article in Journal/Newspaper Antarc* University of Maryland: Digital Repository (DRUM) |
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Open Polar |
collection |
University of Maryland: Digital Repository (DRUM) |
op_collection_id |
ftunivmaryland |
language |
English |
topic |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris |
spellingShingle |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Ranjan, Peeyush Kateriya, Suneel Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
topic_facet |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris |
description |
The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants. https://doi.org/10.1186/s13104-018-3181-4 |
format |
Article in Journal/Newspaper |
author |
Ranjan, Peeyush Kateriya, Suneel |
author_facet |
Ranjan, Peeyush Kateriya, Suneel |
author_sort |
Ranjan, Peeyush |
title |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_short |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_full |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_fullStr |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_full_unstemmed |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_sort |
localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
publisher |
Springer Nature |
publishDate |
2018 |
url |
http://hdl.handle.net/1903/27336 https://doi.org/10.13016/xuor-xohf |
genre |
Antarc* |
genre_facet |
Antarc* |
op_relation |
Cell Biology & Molecular Genetics Digital Repository at the University of Maryland College of Computer, Mathematical & Natural Sciences University of Maryland (College Park, MD) https://doi.org/10.13016/xuor-xohf Ranjan, P., Kateriya, S. Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae. BMC Res Notes 11, 65 (2018). http://hdl.handle.net/1903/27336 |
op_doi |
https://doi.org/10.13016/xuor-xohf |
_version_ |
1766270782399315968 |