Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide
Isoamyl acetate was successfully synthesized from isoamyl alcohol in supercritical carbon dioxide by enzymatic catalysis. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, two diff...
Main Authors: | , , , , , |
---|---|
Format: | Other/Unknown Material |
Language: | English |
Published: |
PRA Press
2012
|
Subjects: | |
Online Access: | https://dk.um.si/IzpisGradiva.php?id=27209 https://dk.um.si/Dokument.php?id=36464&dn= http://www.cobiss.si/scripts/cobiss?command=DISPLAY&base=cobib&rid=9162774&fmt=11 |
id |
ftunivmaribor:oai:dk.um.si:IzpisGradiva.php-id-27209 |
---|---|
record_format |
openpolar |
spelling |
ftunivmaribor:oai:dk.um.si:IzpisGradiva.php-id-27209 2023-05-15T14:02:24+02:00 Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide Romero, M.P. Calvo, L. Alba, C. Habulin, Maja Primožič, Mateja Knez, Željko 2012-06-01 text/url https://dk.um.si/IzpisGradiva.php?id=27209 https://dk.um.si/Dokument.php?id=36464&dn= http://www.cobiss.si/scripts/cobiss?command=DISPLAY&base=cobib&rid=9162774&fmt=11 eng eng PRA Press info:eu-repo/semantics/openAccess The Journal of supercritical fluids, 2005. ISSN: 0896-8446 chemical processing high pressure technology superkritical CO2 ester synthesis isoamyl acetate enzymatic catalysis immobilized lipase isoamyl acetate production natural flavors kemijska procesna tehnika visokotlačna tehnologija superkritični CO2 sinteze estrov izoamil acetat encimska kataliza imobilizirana lipaza proizvodnja izoamil acetata proizvodnja naravnih arom info:eu-repo/classification/udc/66.091-987 info:eu-repo/semantics/other info:eu-repo/semantics/publishedVersion 2012 ftunivmaribor 2019-02-03T16:15:12Z Isoamyl acetate was successfully synthesized from isoamyl alcohol in supercritical carbon dioxide by enzymatic catalysis. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, two different immobilized lipases (Novozym 435 from Candida antarctica and Lipozyme RM-IM from Rhizomucor miehei) as biocatalysts for the above-mentionedreaction were compared. An esterification extent of 100% was obtained in continuous operation using acetic anhydride as acyl donor and Novozym 435 as enzyme. The amount of enzyme preparation was optimised to 6.25 g/mol alcohol. The effect of substrates load in the solvent was investigated. Operating at a CO2žsubstrates molar ratio below 7.0, the conversion of alcoholdecreased, probably due to an inhibitory effect on enzyme by high concentration of acetic anydride or by produced acetic acid. Pressure in the range of 8-30 MPa showed no effect on this reaction, while an increase in temperature (over 313 K) led to lower production of isoamyl acetate. Novozym 435 was very stable not finding any loss of activity during one month of continuous operation. Finally, carbon dioxide was compared to a conventional organic solvent (n-hexane). Initial reaction rate was higher in SC-CO2, although final esterification extent was similar in both media. Other/Unknown Material Antarc* Antarctica Digital Library of University of Maribor |
institution |
Open Polar |
collection |
Digital Library of University of Maribor |
op_collection_id |
ftunivmaribor |
language |
English |
topic |
chemical processing high pressure technology superkritical CO2 ester synthesis isoamyl acetate enzymatic catalysis immobilized lipase isoamyl acetate production natural flavors kemijska procesna tehnika visokotlačna tehnologija superkritični CO2 sinteze estrov izoamil acetat encimska kataliza imobilizirana lipaza proizvodnja izoamil acetata proizvodnja naravnih arom info:eu-repo/classification/udc/66.091-987 |
spellingShingle |
chemical processing high pressure technology superkritical CO2 ester synthesis isoamyl acetate enzymatic catalysis immobilized lipase isoamyl acetate production natural flavors kemijska procesna tehnika visokotlačna tehnologija superkritični CO2 sinteze estrov izoamil acetat encimska kataliza imobilizirana lipaza proizvodnja izoamil acetata proizvodnja naravnih arom info:eu-repo/classification/udc/66.091-987 Romero, M.P. Calvo, L. Alba, C. Habulin, Maja Primožič, Mateja Knez, Željko Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
topic_facet |
chemical processing high pressure technology superkritical CO2 ester synthesis isoamyl acetate enzymatic catalysis immobilized lipase isoamyl acetate production natural flavors kemijska procesna tehnika visokotlačna tehnologija superkritični CO2 sinteze estrov izoamil acetat encimska kataliza imobilizirana lipaza proizvodnja izoamil acetata proizvodnja naravnih arom info:eu-repo/classification/udc/66.091-987 |
description |
Isoamyl acetate was successfully synthesized from isoamyl alcohol in supercritical carbon dioxide by enzymatic catalysis. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, two different immobilized lipases (Novozym 435 from Candida antarctica and Lipozyme RM-IM from Rhizomucor miehei) as biocatalysts for the above-mentionedreaction were compared. An esterification extent of 100% was obtained in continuous operation using acetic anhydride as acyl donor and Novozym 435 as enzyme. The amount of enzyme preparation was optimised to 6.25 g/mol alcohol. The effect of substrates load in the solvent was investigated. Operating at a CO2žsubstrates molar ratio below 7.0, the conversion of alcoholdecreased, probably due to an inhibitory effect on enzyme by high concentration of acetic anydride or by produced acetic acid. Pressure in the range of 8-30 MPa showed no effect on this reaction, while an increase in temperature (over 313 K) led to lower production of isoamyl acetate. Novozym 435 was very stable not finding any loss of activity during one month of continuous operation. Finally, carbon dioxide was compared to a conventional organic solvent (n-hexane). Initial reaction rate was higher in SC-CO2, although final esterification extent was similar in both media. |
format |
Other/Unknown Material |
author |
Romero, M.P. Calvo, L. Alba, C. Habulin, Maja Primožič, Mateja Knez, Željko |
author_facet |
Romero, M.P. Calvo, L. Alba, C. Habulin, Maja Primožič, Mateja Knez, Željko |
author_sort |
Romero, M.P. |
title |
Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
title_short |
Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
title_full |
Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
title_fullStr |
Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
title_full_unstemmed |
Enzymatic synthesis of isoamyl acetate with immobilized Candida antarctica lipase in supercritical carbon dioxide |
title_sort |
enzymatic synthesis of isoamyl acetate with immobilized candida antarctica lipase in supercritical carbon dioxide |
publisher |
PRA Press |
publishDate |
2012 |
url |
https://dk.um.si/IzpisGradiva.php?id=27209 https://dk.um.si/Dokument.php?id=36464&dn= http://www.cobiss.si/scripts/cobiss?command=DISPLAY&base=cobib&rid=9162774&fmt=11 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
The Journal of supercritical fluids, 2005. ISSN: 0896-8446 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766272670620450816 |