Nanoencapsulation-based delivery technologies for Salmo salar by-products derived protein hydrolysates

• Main Author: Jayasinghe Mudiyanselage, Janani
• Other Authors: Aluko, Rotimi (Food and Human Nutritional Sciences), Malalgoda, Maneka (Food and Human Nutritional Sciences), Bandara, Nandika, Mason, Beth
• Format: Master Thesis
• Language: English
• Published: 2023
• Subjects: Nanoemulsion; Nanoparticles; Salmon protein hydrolysates; Encapsulation efficiency; Release during inviro digestion; Salmo salar
• Online Access: http://hdl.handle.net/1993/37473

By-products derived protein hydrolysates from Salmo salar are known to have important bioactivities such as antioxidative activity. However, their application in the food industry has been limited for a few reasons, including low bioavailability, low stability, heterogenous nature, interaction with food matrix and hydrophobicity. Nanoencapsulation can be employed as a strategy to overcome the above challenges that are faced by the peptides. Different types of carriers can be used for nanoencapsulation, including lipid-based, polysaccharide-based and protein-based. So this study consisted of two different delivery techniques developed for S.salar by-products derived protein hydrolysates (SPH). The objective of the first study was to optimize the process conditions (aqueous phase % in primary water in oil emulsion (W), aqueous phase% in w/o/w emulsion (W/O), PGPR 90% and Tween 80%) for W/O/W nanoemulsion preparation to encapsulate SPH. A Box-Benkan design was used to optimize the conditions for the emulsion preparation. Optimized conditions for emulsion preparation: W% =20%, W/O = 20%, PGPR = 8% and Tween 80 = 2.6% were obtained respectively. Experimented particle size and EE values under these conditions were 285.5 nm and 59%, respectively. Optimization of a double emulsion for encapsulating was achieved. Optimized emulsion and coated emulsions showed good storage ability over 28 days period. And the objective of the second study was to optimize the process conditions for chitosan/alginate nanoparticles (CS/AL NPs) to encapsulate SPH. Independent variables were alginate/chitosan (w/w), alginate/ CaCl2 (w/w) and alginate/ peptides (w/w) with three levels. The optimized nanoparticles’ particle size, zeta potential and encapsulation efficiency (EE) were 536.7 nm, -30.2 mV and 29.8 %, respectively. XRD and FTIR results proved the incorporation of SPH into the CS/AL NPs. Moreover, both systems showed a sustainable release of SPH in the gastrointestinal model conditions. These lipid-based and polysaccharide-based nano ...