Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada
Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable micr...
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ftunivmanitoba:oai:mspace.lib.umanitoba.ca:1993/31870 2023-06-18T03:40:37+02:00 Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada Fernando, Dinesh M. Tun, Hein Min Poole, Jenna Patidar, Rakesh Li, Ru Mi, Ruidong Amarawansha, Geethani E. A. Fernando, W. G. Dilantha Khafipour, Ehsan Farenhorst, Annemieke Kumara, Ayush 2016-08 application/pdf http://hdl.handle.net/1993/31870 eng eng American Society for Microbiology Fernando, D.M., Tun, H.M., Poole, J., Patidar, R., LI, R., MI, R., Amarawansha, G.E.A., Fernando, W.G.D., Khafipour, E., Farenhorst, A., Kumar, A. (2016). Detection of antibiotic resistance genes in source and drinking water samples from a First Nation Community in Canada. Applied and Environmental Microbiology. 82(15): 4767-4775. http://hdl.handle.net/1993/31870 open access drinking water human right antibiotic resistance coliforms Illumina sequencing First Nations reserves drinking water advisory microbiological quality Article 2016 ftunivmanitoba 2023-06-04T17:45:54Z Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, -lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. This work was supported by Discovery (A.K., W.G.D.F.) and CreateH2O (A.F.) grants funded by the Natural Science and Engineering Research Council of Canada as well as a University of Manitoba Start-up grant (E.K.). D.M.F. was funded by a Canada Research Chair grant to Peter Loewen, Department of Microbiology, University of Manitoba. R.P. is funded by a postdoctoral fellowship from the Government of Madhya Pradesh, India. Article in Journal/Newspaper First Nations MSpace at the University of Manitoba Canada |
institution |
Open Polar |
collection |
MSpace at the University of Manitoba |
op_collection_id |
ftunivmanitoba |
language |
English |
topic |
drinking water human right antibiotic resistance coliforms Illumina sequencing First Nations reserves drinking water advisory microbiological quality |
spellingShingle |
drinking water human right antibiotic resistance coliforms Illumina sequencing First Nations reserves drinking water advisory microbiological quality Fernando, Dinesh M. Tun, Hein Min Poole, Jenna Patidar, Rakesh Li, Ru Mi, Ruidong Amarawansha, Geethani E. A. Fernando, W. G. Dilantha Khafipour, Ehsan Farenhorst, Annemieke Kumara, Ayush Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
topic_facet |
drinking water human right antibiotic resistance coliforms Illumina sequencing First Nations reserves drinking water advisory microbiological quality |
description |
Access to safe drinking water is now recognized as a human right by the United Nations. In developed countries like Canada, access to clean water is generally not a matter of concern. However, one in every five First Nations reserves is under a drinking water advisory, often due to unacceptable microbiological quality. In this study, we analyzed source and potable water from a First Nations community for the presence of coliform bacteria as well as various antibiotic resistance genes. Samples, including those from drinking water sources, were found to be positive for various antibiotic resistance genes, namely, ampC, tet(A), mecA, -lactamase genes (SHV-type, TEM-type, CTX-M-type, OXA-1, and CMY-2-type), and carbapenemase genes (KPC, IMP, VIM, NDM, GES, and OXA-48 genes). Not surprisingly, substantial numbers of total coliforms, including Escherichia coli, were recovered from these samples, and this result was also confirmed using Illumina sequencing of the 16S rRNA gene. These findings deserve further attention, as the presence of coliforms and antibiotic resistance genes potentially puts the health of the community members at risk. This work was supported by Discovery (A.K., W.G.D.F.) and CreateH2O (A.F.) grants funded by the Natural Science and Engineering Research Council of Canada as well as a University of Manitoba Start-up grant (E.K.). D.M.F. was funded by a Canada Research Chair grant to Peter Loewen, Department of Microbiology, University of Manitoba. R.P. is funded by a postdoctoral fellowship from the Government of Madhya Pradesh, India. |
format |
Article in Journal/Newspaper |
author |
Fernando, Dinesh M. Tun, Hein Min Poole, Jenna Patidar, Rakesh Li, Ru Mi, Ruidong Amarawansha, Geethani E. A. Fernando, W. G. Dilantha Khafipour, Ehsan Farenhorst, Annemieke Kumara, Ayush |
author_facet |
Fernando, Dinesh M. Tun, Hein Min Poole, Jenna Patidar, Rakesh Li, Ru Mi, Ruidong Amarawansha, Geethani E. A. Fernando, W. G. Dilantha Khafipour, Ehsan Farenhorst, Annemieke Kumara, Ayush |
author_sort |
Fernando, Dinesh M. |
title |
Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
title_short |
Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
title_full |
Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
title_fullStr |
Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
title_full_unstemmed |
Detection of Antibiotic Resistance Genes in Source and Drinking Water Samples from a First Nations Community in Canada |
title_sort |
detection of antibiotic resistance genes in source and drinking water samples from a first nations community in canada |
publisher |
American Society for Microbiology |
publishDate |
2016 |
url |
http://hdl.handle.net/1993/31870 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
First Nations |
genre_facet |
First Nations |
op_relation |
Fernando, D.M., Tun, H.M., Poole, J., Patidar, R., LI, R., MI, R., Amarawansha, G.E.A., Fernando, W.G.D., Khafipour, E., Farenhorst, A., Kumar, A. (2016). Detection of antibiotic resistance genes in source and drinking water samples from a First Nation Community in Canada. Applied and Environmental Microbiology. 82(15): 4767-4775. http://hdl.handle.net/1993/31870 |
op_rights |
open access |
_version_ |
1769005811645808640 |