Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens)
Namao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid...
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Diseases of Aquatic Organisms
2013
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ftunivmanitoba:oai:mspace.lib.umanitoba.ca:1993/30754 2023-06-18T03:41:45+02:00 Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) Van Walleghem, Elissa Clouthier, Sharon (Biological Sciences) Anderson, Gary (Biological Sciences) Docker, Margaret (Biological Sciences) Butler, Micheal (Microbiology) 2013 application/pdf http://hdl.handle.net/1993/30754 eng eng Diseases of Aquatic Organisms Clouthier SC, VanWalleghem E, Copeland S, Klassen C, Hobbs G, Nielsen O, Anderson ED (2013) Namao virus, a new unclassified species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens. Dis Aquat Org 102:195-209 http://hdl.handle.net/1993/30754 open access Namao virus NCLDV Lake Sturgeon Endangered species qPCR Diagnostic PCR Real-time PCR Mimiviridae master thesis 2013 ftunivmanitoba 2023-06-04T17:46:28Z Namao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid protein showed that NV and other epitheliotropic sturgeon nucleo-cytoplasmic large DNA viruses shared a common evolutionary past and formed a distinct evolutionary lineage within Megavirales. Three PCR tests were developed and their analytical performance was validated for detection of these viruses. Testing of wild sturgeon revealed that NV is endemic in the Nelson River water basin in Manitoba. Bath exposure resulted in transmission of NV to healthy sturgeon. The gills appeared to be the initial site of infection with virus persisting in the head skin tissue for up to 62 days. The molecular tests will be useful tools for disease management in sturgeon conservation stocking programs. October 2015 Master Thesis Nelson River MSpace at the University of Manitoba Canada |
institution |
Open Polar |
collection |
MSpace at the University of Manitoba |
op_collection_id |
ftunivmanitoba |
language |
English |
topic |
Namao virus NCLDV Lake Sturgeon Endangered species qPCR Diagnostic PCR Real-time PCR Mimiviridae |
spellingShingle |
Namao virus NCLDV Lake Sturgeon Endangered species qPCR Diagnostic PCR Real-time PCR Mimiviridae Van Walleghem, Elissa Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
topic_facet |
Namao virus NCLDV Lake Sturgeon Endangered species qPCR Diagnostic PCR Real-time PCR Mimiviridae |
description |
Namao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid protein showed that NV and other epitheliotropic sturgeon nucleo-cytoplasmic large DNA viruses shared a common evolutionary past and formed a distinct evolutionary lineage within Megavirales. Three PCR tests were developed and their analytical performance was validated for detection of these viruses. Testing of wild sturgeon revealed that NV is endemic in the Nelson River water basin in Manitoba. Bath exposure resulted in transmission of NV to healthy sturgeon. The gills appeared to be the initial site of infection with virus persisting in the head skin tissue for up to 62 days. The molecular tests will be useful tools for disease management in sturgeon conservation stocking programs. October 2015 |
author2 |
Clouthier, Sharon (Biological Sciences) Anderson, Gary (Biological Sciences) Docker, Margaret (Biological Sciences) Butler, Micheal (Microbiology) |
format |
Master Thesis |
author |
Van Walleghem, Elissa |
author_facet |
Van Walleghem, Elissa |
author_sort |
Van Walleghem, Elissa |
title |
Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
title_short |
Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
title_full |
Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
title_fullStr |
Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
title_full_unstemmed |
Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens) |
title_sort |
analytical performance characteristics and application of diagnostic tests for namao virus in experimentally infected and wild manitoba lake sturgeon (acipenser fulvescens) |
publisher |
Diseases of Aquatic Organisms |
publishDate |
2013 |
url |
http://hdl.handle.net/1993/30754 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Nelson River |
genre_facet |
Nelson River |
op_relation |
Clouthier SC, VanWalleghem E, Copeland S, Klassen C, Hobbs G, Nielsen O, Anderson ED (2013) Namao virus, a new unclassified species of nucleo-cytoplasmic large DNA virus (NCLDV) associated with mortalities in Manitoba lake sturgeon Acipenser fulvescens. Dis Aquat Org 102:195-209 http://hdl.handle.net/1993/30754 |
op_rights |
open access |
_version_ |
1769007411079675904 |