The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat
Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophil...
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Online Access: | http://eprints.utm.my/79945/ http://eprints.utm.my/79945/1/RosliMdIllias2018_TheGlaciozymaAntarcticaGenomeReveals.pdf https://doi.org/10.1371/journal.pone.0189947 |
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ftunivmalaysia:oai:generic.eprints.org:79945 2023-11-12T04:05:23+01:00 The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat Firdaus Raih, M. Hashim, N. H. F. Bharudin, I. Bakar, M. F. A. Huang, K. K. Alias, H. Lee, B. K. B. Isa, M. N. M. Mat-Sharani, S. Sulaiman, S. Tay, L. J. Zolkefli, R. Noor, Y. M. Law, D. S. N. Rahman, S. H. A. Md-Illias, R. Bakar, F. D. A. Najimudin, N. Murad, A. M. A. Mahadi, N. M. 2018-01 application/pdf http://eprints.utm.my/79945/ http://eprints.utm.my/79945/1/RosliMdIllias2018_TheGlaciozymaAntarcticaGenomeReveals.pdf https://doi.org/10.1371/journal.pone.0189947 en eng Public Library of Science http://eprints.utm.my/79945/1/RosliMdIllias2018_TheGlaciozymaAntarcticaGenomeReveals.pdf Firdaus Raih, M. and Hashim, N. H. F. and Bharudin, I. and Bakar, M. F. A. and Huang, K. K. and Alias, H. and Lee, B. K. B. and Isa, M. N. M. and Mat-Sharani, S. and Sulaiman, S. and Tay, L. J. and Zolkefli, R. and Noor, Y. M. and Law, D. S. N. and Rahman, S. H. A. and Md-Illias, R. and Bakar, F. D. A. and Najimudin, N. and Murad, A. M. A. and Mahadi, N. M. (2018) The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. PLoS ONE, 13 (1). ISSN 1932-6203 TP Chemical technology Article PeerReviewed 2018 ftunivmalaysia https://doi.org/10.1371/journal.pone.0189947 2023-10-24T17:59:55Z Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. Article in Journal/Newspaper Antarc* Antarctic Antarctica Universiti Teknologi Malaysia: Institutional Repository Antarctic The Antarctic PLOS ONE 13 1 e0189947 |
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Open Polar |
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Universiti Teknologi Malaysia: Institutional Repository |
op_collection_id |
ftunivmalaysia |
language |
English |
topic |
TP Chemical technology |
spellingShingle |
TP Chemical technology Firdaus Raih, M. Hashim, N. H. F. Bharudin, I. Bakar, M. F. A. Huang, K. K. Alias, H. Lee, B. K. B. Isa, M. N. M. Mat-Sharani, S. Sulaiman, S. Tay, L. J. Zolkefli, R. Noor, Y. M. Law, D. S. N. Rahman, S. H. A. Md-Illias, R. Bakar, F. D. A. Najimudin, N. Murad, A. M. A. Mahadi, N. M. The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
topic_facet |
TP Chemical technology |
description |
Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. |
format |
Article in Journal/Newspaper |
author |
Firdaus Raih, M. Hashim, N. H. F. Bharudin, I. Bakar, M. F. A. Huang, K. K. Alias, H. Lee, B. K. B. Isa, M. N. M. Mat-Sharani, S. Sulaiman, S. Tay, L. J. Zolkefli, R. Noor, Y. M. Law, D. S. N. Rahman, S. H. A. Md-Illias, R. Bakar, F. D. A. Najimudin, N. Murad, A. M. A. Mahadi, N. M. |
author_facet |
Firdaus Raih, M. Hashim, N. H. F. Bharudin, I. Bakar, M. F. A. Huang, K. K. Alias, H. Lee, B. K. B. Isa, M. N. M. Mat-Sharani, S. Sulaiman, S. Tay, L. J. Zolkefli, R. Noor, Y. M. Law, D. S. N. Rahman, S. H. A. Md-Illias, R. Bakar, F. D. A. Najimudin, N. Murad, A. M. A. Mahadi, N. M. |
author_sort |
Firdaus Raih, M. |
title |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
title_short |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
title_full |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
title_fullStr |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
title_full_unstemmed |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
title_sort |
glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
http://eprints.utm.my/79945/ http://eprints.utm.my/79945/1/RosliMdIllias2018_TheGlaciozymaAntarcticaGenomeReveals.pdf https://doi.org/10.1371/journal.pone.0189947 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://eprints.utm.my/79945/1/RosliMdIllias2018_TheGlaciozymaAntarcticaGenomeReveals.pdf Firdaus Raih, M. and Hashim, N. H. F. and Bharudin, I. and Bakar, M. F. A. and Huang, K. K. and Alias, H. and Lee, B. K. B. and Isa, M. N. M. and Mat-Sharani, S. and Sulaiman, S. and Tay, L. J. and Zolkefli, R. and Noor, Y. M. and Law, D. S. N. and Rahman, S. H. A. and Md-Illias, R. and Bakar, F. D. A. and Najimudin, N. and Murad, A. M. A. and Mahadi, N. M. (2018) The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. PLoS ONE, 13 (1). ISSN 1932-6203 |
op_doi |
https://doi.org/10.1371/journal.pone.0189947 |
container_title |
PLOS ONE |
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13 |
container_issue |
1 |
container_start_page |
e0189947 |
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1782341969368842240 |