Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications
One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is dif...
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ftunivmalaysia:oai:generic.eprints.org:74689 2023-05-15T13:31:42+02:00 Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. 2013 http://eprints.utm.my/74689/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930329366&doi=10.1007%2f978-3-642-39681-6_22&partnerID=40&md5=5dc62ec0fe438c04834587cf4c24180a unknown Springer-Verlag Berlin Heidelberg Illias, R. M. and Ramli, A. N. M. and Low, K. O. and Muhammad Mahadi, N. and Abdul Murad, A. M. and Rabu, A. (2013) Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications. In: Cold-adapted Yeasts: Biodiversity, Adaptation Strategies and Biotechnological Significance. Springer-Verlag Berlin Heidelberg, pp. 481-496. ISBN 978-364239681-6 TP Chemical technology Book Section PeerReviewed 2013 ftunivmalaysia 2017-11-28T15:58:25Z One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is difficult. For each protein, a suitable expression system must be optimised to obtain the sample in a soluble, correctly folded conformation with high production yield. The recent discovery of psychrophilic yeasts and their potential in industrial applications have sparked interest in overexpression strategies for high-level expression of psychrophilic proteins. Here, we discuss some of the recent developments in the recombinant production of cold-adapted yeast proteins, particularly those from Glaciozyma antarctica PI12, in suitable heterologous hosts. Findings from our work, as well as from recent publications, are included. Book Part Antarc* Antarctica Universiti Teknologi Malaysia: Institutional Repository |
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Universiti Teknologi Malaysia: Institutional Repository |
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ftunivmalaysia |
language |
unknown |
topic |
TP Chemical technology |
spellingShingle |
TP Chemical technology Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
topic_facet |
TP Chemical technology |
description |
One of the prerequisites for the functional and structural characterisation of proteins is to obtain a sufficiently high amount of the protein sample. Recombinant protein expression systems are usually employed for the overproduction of proteins in cases where isolation from their native host is difficult. For each protein, a suitable expression system must be optimised to obtain the sample in a soluble, correctly folded conformation with high production yield. The recent discovery of psychrophilic yeasts and their potential in industrial applications have sparked interest in overexpression strategies for high-level expression of psychrophilic proteins. Here, we discuss some of the recent developments in the recombinant production of cold-adapted yeast proteins, particularly those from Glaciozyma antarctica PI12, in suitable heterologous hosts. Findings from our work, as well as from recent publications, are included. |
format |
Book Part |
author |
Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. |
author_facet |
Illias, R. M. Ramli, A. N. M. Low, K. O. Muhammad Mahadi, N. Abdul Murad, A. M. Rabu, A. |
author_sort |
Illias, R. M. |
title |
Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
title_short |
Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
title_full |
Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
title_fullStr |
Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
title_full_unstemmed |
Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
title_sort |
heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications |
publisher |
Springer-Verlag Berlin Heidelberg |
publishDate |
2013 |
url |
http://eprints.utm.my/74689/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930329366&doi=10.1007%2f978-3-642-39681-6_22&partnerID=40&md5=5dc62ec0fe438c04834587cf4c24180a |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Illias, R. M. and Ramli, A. N. M. and Low, K. O. and Muhammad Mahadi, N. and Abdul Murad, A. M. and Rabu, A. (2013) Heterologous expression of proteins from cold-adapted yeasts in suitable hosts: methods and applications. In: Cold-adapted Yeasts: Biodiversity, Adaptation Strategies and Biotechnological Significance. Springer-Verlag Berlin Heidelberg, pp. 481-496. ISBN 978-364239681-6 |
_version_ |
1766020399365095424 |