Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12
The psychrophilic yeast Glaciozyma antarctica demonstrated high antifreeze activity in its culture filtrate. The culture filtrate exhibited both thermal hysteresis (TH) and ice recrystallization inhibition (RI) properties. The TH of 0.1 °C was comparable to that previously reported for bacteria and...
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Online Access: | http://eprints.utm.my/49153/ https://doi.org/10.1007/s00792-012-0494-4 |
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ftunivmalaysia:oai:generic.eprints.org:49153 2023-11-12T04:07:09+01:00 Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 Hashim, Noor Haza Fazlin Bharudin, Izwan Law, Douglas Sie Nguong Higa, Sakura Abu Bakar, Farah Diba Nathan, Sheila Rabu, Amir Kawahara, Hidehisa Md. Illias, Rosli Najimudin, Nazalan Mahadi, Nor Muhammad Abdul Murad, Abdul Munir 2013 http://eprints.utm.my/49153/ https://doi.org/10.1007/s00792-012-0494-4 unknown Springer Hashim, Noor Haza Fazlin and Bharudin, Izwan and Law, Douglas Sie Nguong and Higa, Sakura and Abu Bakar, Farah Diba and Nathan, Sheila and Rabu, Amir and Kawahara, Hidehisa and Md. Illias, Rosli and Najimudin, Nazalan and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir (2013) Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12. Extremophiles, 17 (1). pp. 63-73. ISSN 1431-0651 QD Chemistry Article PeerReviewed 2013 ftunivmalaysia https://doi.org/10.1007/s00792-012-0494-4 2023-10-24T17:52:28Z The psychrophilic yeast Glaciozyma antarctica demonstrated high antifreeze activity in its culture filtrate. The culture filtrate exhibited both thermal hysteresis (TH) and ice recrystallization inhibition (RI) properties. The TH of 0.1 °C was comparable to that previously reported for bacteria and fungi. A genome sequence survey of the G. antarctica genome identified a novel antifreeze protein gene. The cDNA encoded a 177 amino acid protein with 30 % similarity to a fungal antifreeze protein from Typhula ishikariensis. The expression levels of AFP1 were quantified via real time-quantitative polymerase chain reaction (RT-qPCR), and the highest expression levels were detected within 6 h of growth at -12 °C. The cDNA of the antifreeze protein was cloned into an Escherichia coli expression system. Expression of recombinant Afp1 in E. coli resulted in the formation of inclusion bodies that were subsequently denatured by treatment with urea and allowed to refold in vitro. Activity assays of the recombinant Afp1 confirmed the antifreeze protein properties with a high TH value of 0.08 °C. Article in Journal/Newspaper Antarc* Antarctica Universiti Teknologi Malaysia: Institutional Repository Extremophiles 17 1 63 73 |
institution |
Open Polar |
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Universiti Teknologi Malaysia: Institutional Repository |
op_collection_id |
ftunivmalaysia |
language |
unknown |
topic |
QD Chemistry |
spellingShingle |
QD Chemistry Hashim, Noor Haza Fazlin Bharudin, Izwan Law, Douglas Sie Nguong Higa, Sakura Abu Bakar, Farah Diba Nathan, Sheila Rabu, Amir Kawahara, Hidehisa Md. Illias, Rosli Najimudin, Nazalan Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
topic_facet |
QD Chemistry |
description |
The psychrophilic yeast Glaciozyma antarctica demonstrated high antifreeze activity in its culture filtrate. The culture filtrate exhibited both thermal hysteresis (TH) and ice recrystallization inhibition (RI) properties. The TH of 0.1 °C was comparable to that previously reported for bacteria and fungi. A genome sequence survey of the G. antarctica genome identified a novel antifreeze protein gene. The cDNA encoded a 177 amino acid protein with 30 % similarity to a fungal antifreeze protein from Typhula ishikariensis. The expression levels of AFP1 were quantified via real time-quantitative polymerase chain reaction (RT-qPCR), and the highest expression levels were detected within 6 h of growth at -12 °C. The cDNA of the antifreeze protein was cloned into an Escherichia coli expression system. Expression of recombinant Afp1 in E. coli resulted in the formation of inclusion bodies that were subsequently denatured by treatment with urea and allowed to refold in vitro. Activity assays of the recombinant Afp1 confirmed the antifreeze protein properties with a high TH value of 0.08 °C. |
format |
Article in Journal/Newspaper |
author |
Hashim, Noor Haza Fazlin Bharudin, Izwan Law, Douglas Sie Nguong Higa, Sakura Abu Bakar, Farah Diba Nathan, Sheila Rabu, Amir Kawahara, Hidehisa Md. Illias, Rosli Najimudin, Nazalan Mahadi, Nor Muhammad Abdul Murad, Abdul Munir |
author_facet |
Hashim, Noor Haza Fazlin Bharudin, Izwan Law, Douglas Sie Nguong Higa, Sakura Abu Bakar, Farah Diba Nathan, Sheila Rabu, Amir Kawahara, Hidehisa Md. Illias, Rosli Najimudin, Nazalan Mahadi, Nor Muhammad Abdul Murad, Abdul Munir |
author_sort |
Hashim, Noor Haza Fazlin |
title |
Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
title_short |
Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
title_full |
Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
title_fullStr |
Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
title_full_unstemmed |
Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12 |
title_sort |
characterization of afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica pi12 |
publisher |
Springer |
publishDate |
2013 |
url |
http://eprints.utm.my/49153/ https://doi.org/10.1007/s00792-012-0494-4 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Hashim, Noor Haza Fazlin and Bharudin, Izwan and Law, Douglas Sie Nguong and Higa, Sakura and Abu Bakar, Farah Diba and Nathan, Sheila and Rabu, Amir and Kawahara, Hidehisa and Md. Illias, Rosli and Najimudin, Nazalan and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir (2013) Characterization of Afp1, an antifreeze protein from the psychrophilic yeast glaciozyma antarctica PI12. Extremophiles, 17 (1). pp. 63-73. ISSN 1431-0651 |
op_doi |
https://doi.org/10.1007/s00792-012-0494-4 |
container_title |
Extremophiles |
container_volume |
17 |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
73 |
_version_ |
1782327951070593024 |