Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis
This work reviews the stripping off, role of water molecules in activity, and flexibility of immobilized Candida antarctica lipase B (CALB). Employment of CALB in ring opening polyester synthesis emphasizing on a polylactide is discussed in detail. Execution of enzymes in place of inorganic catalyst...
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Online Access: | http://eprints.utm.my/47075/ https://doi.org/10.1016/j.biotechadv.2011.10.002 |
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ftunivmalaysia:oai:generic.eprints.org:47075 2023-11-12T04:07:16+01:00 Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis Idris, Ani Bukhari, Attaullah 2012 http://eprints.utm.my/47075/ https://doi.org/10.1016/j.biotechadv.2011.10.002 unknown Elsevier Ltd. Idris, Ani and Bukhari, Attaullah (2012) Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis. Biotechnology Advances, 30 (3). pp. 550-563. ISSN 0734-9750 TP Chemical technology Article PeerReviewed 2012 ftunivmalaysia https://doi.org/10.1016/j.biotechadv.2011.10.002 2023-10-24T17:51:46Z This work reviews the stripping off, role of water molecules in activity, and flexibility of immobilized Candida antarctica lipase B (CALB). Employment of CALB in ring opening polyester synthesis emphasizing on a polylactide is discussed in detail. Execution of enzymes in place of inorganic catalysts is the most green alternative for sustainable and environment friendly synthesis of products on an industrial scale. Robust immobilization and consequently performance of enzyme is the essential objective of enzyme application in industry. Water bound to the surface of an enzyme (contact class of water molecules) is inevitable for enzyme performance; it controls enzyme dynamics via flexibility changes and has intensive influence on enzyme activity. The value of pH during immobilization of CALB plays a critical role in fixing the active conformation of an enzyme. Comprehensive selection of support and protocol can develop a robust immobilized enzyme thus enhancing its performance. Organic solvents with a log P value higher than four are more suitable for enzymatic catalysis as these solvents tend to strip away very little of the enzyme surface bound water molecules. Alternatively ionic liquid can work as a more promising reaction media. Covalent immobilization is an exclusively reliable technique to circumvent the leaching of enzymes and to enhance stability. Activated polystyrene nanoparticles can prove to be a practical and economical support for chemical immobilization of CALB. In order to reduce the E-factor for the synthesis of biodegradable polymers; enzymatic ring opening polyester synthesis (eROPS) of cyclic monomers is a more sensible route for polyester synthesis. Synergies obtained from ionic liquids and immobilized enzyme can be much effective eROPS. Article in Journal/Newspaper Antarc* Antarctica antartic* Universiti Teknologi Malaysia: Institutional Repository Biotechnology Advances 30 3 550 563 |
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Universiti Teknologi Malaysia: Institutional Repository |
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ftunivmalaysia |
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unknown |
topic |
TP Chemical technology |
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TP Chemical technology Idris, Ani Bukhari, Attaullah Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
topic_facet |
TP Chemical technology |
description |
This work reviews the stripping off, role of water molecules in activity, and flexibility of immobilized Candida antarctica lipase B (CALB). Employment of CALB in ring opening polyester synthesis emphasizing on a polylactide is discussed in detail. Execution of enzymes in place of inorganic catalysts is the most green alternative for sustainable and environment friendly synthesis of products on an industrial scale. Robust immobilization and consequently performance of enzyme is the essential objective of enzyme application in industry. Water bound to the surface of an enzyme (contact class of water molecules) is inevitable for enzyme performance; it controls enzyme dynamics via flexibility changes and has intensive influence on enzyme activity. The value of pH during immobilization of CALB plays a critical role in fixing the active conformation of an enzyme. Comprehensive selection of support and protocol can develop a robust immobilized enzyme thus enhancing its performance. Organic solvents with a log P value higher than four are more suitable for enzymatic catalysis as these solvents tend to strip away very little of the enzyme surface bound water molecules. Alternatively ionic liquid can work as a more promising reaction media. Covalent immobilization is an exclusively reliable technique to circumvent the leaching of enzymes and to enhance stability. Activated polystyrene nanoparticles can prove to be a practical and economical support for chemical immobilization of CALB. In order to reduce the E-factor for the synthesis of biodegradable polymers; enzymatic ring opening polyester synthesis (eROPS) of cyclic monomers is a more sensible route for polyester synthesis. Synergies obtained from ionic liquids and immobilized enzyme can be much effective eROPS. |
format |
Article in Journal/Newspaper |
author |
Idris, Ani Bukhari, Attaullah |
author_facet |
Idris, Ani Bukhari, Attaullah |
author_sort |
Idris, Ani |
title |
Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
title_short |
Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
title_full |
Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
title_fullStr |
Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
title_full_unstemmed |
Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
title_sort |
immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis |
publisher |
Elsevier Ltd. |
publishDate |
2012 |
url |
http://eprints.utm.my/47075/ https://doi.org/10.1016/j.biotechadv.2011.10.002 |
genre |
Antarc* Antarctica antartic* |
genre_facet |
Antarc* Antarctica antartic* |
op_relation |
Idris, Ani and Bukhari, Attaullah (2012) Immobilized candida antartica lipase b: hydration, stripping off and application in ring opening polyester synthesis. Biotechnology Advances, 30 (3). pp. 550-563. ISSN 0734-9750 |
op_doi |
https://doi.org/10.1016/j.biotechadv.2011.10.002 |
container_title |
Biotechnology Advances |
container_volume |
30 |
container_issue |
3 |
container_start_page |
550 |
op_container_end_page |
563 |
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1782328014330134528 |