Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches
Gelatin from salmon (Salmo salar) skin with high molecular weight protein chains (a-chains) was extracted using trypsin-aided process. Response surface methodology was used to optimise the extraction parameters. Yield, hydroxyproline content and protein electrophoretic profile via sodium dodecyl sul...
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Language: | English |
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Association of Food Scientists & Technologists (India)
2017
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Online Access: | http://eprints.ums.edu.my/id/eprint/31013/ http://eprints.ums.edu.my/id/eprint/31013/1/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process.pdf http://eprints.ums.edu.my/id/eprint/31013/2/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process1.pdf https://link.springer.com/content/pdf/10.1007/s13197-017-2864-5.pdf https://doi.org/10.1007/s13197-017-2864-5 |
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ftunivmalaysab:oai:eprints.ums.edu.my:31013 2023-05-15T18:09:45+02:00 Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches Fan, Hui Yin Marie-Jose´e Dumont Benjamin K. Simpson 2017-11 text http://eprints.ums.edu.my/id/eprint/31013/ http://eprints.ums.edu.my/id/eprint/31013/1/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process.pdf http://eprints.ums.edu.my/id/eprint/31013/2/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process1.pdf https://link.springer.com/content/pdf/10.1007/s13197-017-2864-5.pdf https://doi.org/10.1007/s13197-017-2864-5 en eng Association of Food Scientists & Technologists (India) http://eprints.ums.edu.my/id/eprint/31013/1/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process.pdf http://eprints.ums.edu.my/id/eprint/31013/2/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process1.pdf Fan, Hui Yin and Marie-Jose´e Dumont and Benjamin K. Simpson (2017) Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches. Journal of Food Science and Technology, 54. pp. 4000-4008. ISSN 0975-8402 SH334.9-336.5 Fishery processing Article PeerReviewed 2017 ftunivmalaysab https://doi.org/10.1007/s13197-017-2864-5 2022-03-29T15:45:42Z Gelatin from salmon (Salmo salar) skin with high molecular weight protein chains (a-chains) was extracted using trypsin-aided process. Response surface methodology was used to optimise the extraction parameters. Yield, hydroxyproline content and protein electrophoretic profile via sodium dodecyl sulfate– polyacrylamide gel electrophoresis analysis of gelatin were used as responses in the optimization study. The optimum conditions were determined as: trypsin concentration at 1.49 U/g; extraction temperature at 45 C; and extraction time at 6 h 16 min. This response surface optimized model was significant and produced an experimental value (202.04 ± 8.64%) in good agreement with the predicted value (204.19%). Twofold higher yields of gelatin with high molecular weight protein chains were achieved in the optimized process with trypsin treatment when compared to the process without trypsin. Article in Journal/Newspaper Salmo salar Universiti Malaysia Sabah: UMS Institutional Repository Journal of Food Science and Technology 54 12 4000 4008 |
institution |
Open Polar |
collection |
Universiti Malaysia Sabah: UMS Institutional Repository |
op_collection_id |
ftunivmalaysab |
language |
English |
topic |
SH334.9-336.5 Fishery processing |
spellingShingle |
SH334.9-336.5 Fishery processing Fan, Hui Yin Marie-Jose´e Dumont Benjamin K. Simpson Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
topic_facet |
SH334.9-336.5 Fishery processing |
description |
Gelatin from salmon (Salmo salar) skin with high molecular weight protein chains (a-chains) was extracted using trypsin-aided process. Response surface methodology was used to optimise the extraction parameters. Yield, hydroxyproline content and protein electrophoretic profile via sodium dodecyl sulfate– polyacrylamide gel electrophoresis analysis of gelatin were used as responses in the optimization study. The optimum conditions were determined as: trypsin concentration at 1.49 U/g; extraction temperature at 45 C; and extraction time at 6 h 16 min. This response surface optimized model was significant and produced an experimental value (202.04 ± 8.64%) in good agreement with the predicted value (204.19%). Twofold higher yields of gelatin with high molecular weight protein chains were achieved in the optimized process with trypsin treatment when compared to the process without trypsin. |
format |
Article in Journal/Newspaper |
author |
Fan, Hui Yin Marie-Jose´e Dumont Benjamin K. Simpson |
author_facet |
Fan, Hui Yin Marie-Jose´e Dumont Benjamin K. Simpson |
author_sort |
Fan, Hui Yin |
title |
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
title_short |
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
title_full |
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
title_fullStr |
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
title_full_unstemmed |
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches |
title_sort |
extraction of gelatin from salmon (salmo salar) fish skin using trypsin-aided process: optimization by plackett–burman and response surface methodological approaches |
publisher |
Association of Food Scientists & Technologists (India) |
publishDate |
2017 |
url |
http://eprints.ums.edu.my/id/eprint/31013/ http://eprints.ums.edu.my/id/eprint/31013/1/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process.pdf http://eprints.ums.edu.my/id/eprint/31013/2/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process1.pdf https://link.springer.com/content/pdf/10.1007/s13197-017-2864-5.pdf https://doi.org/10.1007/s13197-017-2864-5 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
http://eprints.ums.edu.my/id/eprint/31013/1/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process.pdf http://eprints.ums.edu.my/id/eprint/31013/2/Extraction%20of%20gelatin%20from%20salmon%20%28Salmo%20salar%29%20fish%20skin%20using%20trypsin-aided%20process1.pdf Fan, Hui Yin and Marie-Jose´e Dumont and Benjamin K. Simpson (2017) Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches. Journal of Food Science and Technology, 54. pp. 4000-4008. ISSN 0975-8402 |
op_doi |
https://doi.org/10.1007/s13197-017-2864-5 |
container_title |
Journal of Food Science and Technology |
container_volume |
54 |
container_issue |
12 |
container_start_page |
4000 |
op_container_end_page |
4008 |
_version_ |
1766182410598219776 |