Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium
The search for new antibiotics is a continuous effort since its discovery. Nevertheless, the speed of discovering novels antibiotics cannot match the speed of bacteria acquiring antibiotics resistant. Hence, the search efforts have broadened to include all compounds with antimicrobial activities. Th...
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ftunivmalaysab:oai:eprints.ums.edu.my:29222 2023-05-15T13:35:53+02:00 Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium S Salister Nur Athirah Yusof Clemente Michael Wong Vui Ling 2019 text http://eprints.ums.edu.my/id/eprint/29222/ http://eprints.ums.edu.my/id/eprint/29222/1/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium.pdf http://eprints.ums.edu.my/id/eprint/29222/2/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium1.pdf https://iopscience.iop.org/article/10.1088/1742-6596/1358/1/012024/pdf en eng IOP Science http://eprints.ums.edu.my/id/eprint/29222/1/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium.pdf http://eprints.ums.edu.my/id/eprint/29222/2/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium1.pdf S Salister and Nur Athirah Yusof and Clemente Michael Wong Vui Ling (2019) Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium. In: 12th Seminar on Science and Technology. Q Science (General) QR Microbiology Conference or Workshop Item PeerReviewed 2019 ftunivmalaysab 2022-03-29T15:44:04Z The search for new antibiotics is a continuous effort since its discovery. Nevertheless, the speed of discovering novels antibiotics cannot match the speed of bacteria acquiring antibiotics resistant. Hence, the search efforts have broadened to include all compounds with antimicrobial activities. The toxin-antitoxin (TA) gene products are the potential antimicrobial compounds worth analyzing. The TA system consists of a set gene found either in the chromosome or plasmid, or both. At the moment, the toxin, a peptide from this system is known to kill some hosts that either encountered stress or have lost the plasmid carrying the TA genes. In a previous study, it was found that an Antarctic bacterium, Cryobacterium sp. SO1 chromosome harbored a putative antimicrobial peptide-coding gene similar to a class II TA, pemK gene. However, it is not clear whether this antimicrobial peptide has cross-species antimicrobial activity. Therefore, this work aims to determine whether this PemK protein has antimicrobial properties or not. The pemKcryobacSo1 gene was ligated to an arabinose-inducible promoter of the Topo pBAD plasmid and used to transform the Escherichia coli TOP10. The cloning of pemK inhibited the growth of the host E. coli TOP10 as the cells failed to grow. This indicated that PemK probably has a cross-species activity inhibited the growth of E. coli apart from its original host Cryobacterium sp. SO1. Conference Object Antarc* Antarctic Universiti Malaysia Sabah: UMS Institutional Repository Antarctic |
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Universiti Malaysia Sabah: UMS Institutional Repository |
op_collection_id |
ftunivmalaysab |
language |
English |
topic |
Q Science (General) QR Microbiology |
spellingShingle |
Q Science (General) QR Microbiology S Salister Nur Athirah Yusof Clemente Michael Wong Vui Ling Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
topic_facet |
Q Science (General) QR Microbiology |
description |
The search for new antibiotics is a continuous effort since its discovery. Nevertheless, the speed of discovering novels antibiotics cannot match the speed of bacteria acquiring antibiotics resistant. Hence, the search efforts have broadened to include all compounds with antimicrobial activities. The toxin-antitoxin (TA) gene products are the potential antimicrobial compounds worth analyzing. The TA system consists of a set gene found either in the chromosome or plasmid, or both. At the moment, the toxin, a peptide from this system is known to kill some hosts that either encountered stress or have lost the plasmid carrying the TA genes. In a previous study, it was found that an Antarctic bacterium, Cryobacterium sp. SO1 chromosome harbored a putative antimicrobial peptide-coding gene similar to a class II TA, pemK gene. However, it is not clear whether this antimicrobial peptide has cross-species antimicrobial activity. Therefore, this work aims to determine whether this PemK protein has antimicrobial properties or not. The pemKcryobacSo1 gene was ligated to an arabinose-inducible promoter of the Topo pBAD plasmid and used to transform the Escherichia coli TOP10. The cloning of pemK inhibited the growth of the host E. coli TOP10 as the cells failed to grow. This indicated that PemK probably has a cross-species activity inhibited the growth of E. coli apart from its original host Cryobacterium sp. SO1. |
format |
Conference Object |
author |
S Salister Nur Athirah Yusof Clemente Michael Wong Vui Ling |
author_facet |
S Salister Nur Athirah Yusof Clemente Michael Wong Vui Ling |
author_sort |
S Salister |
title |
Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
title_short |
Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
title_full |
Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
title_fullStr |
Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
title_full_unstemmed |
Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium |
title_sort |
characterization of a putative antimicrobial peptide from an antarctic bacterium |
publisher |
IOP Science |
publishDate |
2019 |
url |
http://eprints.ums.edu.my/id/eprint/29222/ http://eprints.ums.edu.my/id/eprint/29222/1/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium.pdf http://eprints.ums.edu.my/id/eprint/29222/2/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium1.pdf https://iopscience.iop.org/article/10.1088/1742-6596/1358/1/012024/pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://eprints.ums.edu.my/id/eprint/29222/1/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium.pdf http://eprints.ums.edu.my/id/eprint/29222/2/Characterization%20of%20a%20putative%20antimicrobial%20peptide%20from%20an%20Antarctic%20bacterium1.pdf S Salister and Nur Athirah Yusof and Clemente Michael Wong Vui Ling (2019) Characterization of a Putative Antimicrobial Peptide from an Antarctic Bacterium. In: 12th Seminar on Science and Technology. |
_version_ |
1766071567116140544 |