A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12
Glaciozyma antarctica PI12, a psychrophilic yeast from Antarctica, grows well at low temperatures. However, it is not clear how it responds and adapts to cold and freeze stresses. Hence, this project was set out to determine the cold-adaptation strategies and mechanisms of G. antarctica PI12 using a...
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ftunivmalaysab:oai:eprints.ums.edu.my:27570 2023-05-15T13:35:53+02:00 A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 Wong, Clemente Michael Vui Ling Sook, Yee Boo Voo, Christopher Lok Yung Nursyafiqi Zainuddin Nazalan Najimudin 2019 text http://eprints.ums.edu.my/id/eprint/27570/ http://eprints.ums.edu.my/id/eprint/27570/1/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20FULL%20TEXT.pdf http://eprints.ums.edu.my/id/eprint/27570/2/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20ABSTRACT.pdf https://link.springer.com/content/pdf/10.1007/s00300-018-02443-7.pdf en eng Springer Berlin Heidelberg http://eprints.ums.edu.my/id/eprint/27570/1/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20FULL%20TEXT.pdf http://eprints.ums.edu.my/id/eprint/27570/2/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20ABSTRACT.pdf Wong, Clemente Michael Vui Ling and Sook, Yee Boo and Voo, Christopher Lok Yung and Nursyafiqi Zainuddin and Nazalan Najimudin (2019) A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12. Polar Biology, 42. pp. 541-553. ISSN 1432-2056 QR Microbiology Article PeerReviewed 2019 ftunivmalaysab 2022-03-29T15:43:47Z Glaciozyma antarctica PI12, a psychrophilic yeast from Antarctica, grows well at low temperatures. However, it is not clear how it responds and adapts to cold and freeze stresses. Hence, this project was set out to determine the cold-adaptation strategies and mechanisms of G. antarctica PI12 using a transcriptomic analysis approach. G. antarctica PI12 cells, grown in rich medium at 12 °C, were exposed to freeze stress at 0 and − 12 °C for 6 h and 24 h. Their transcriptomes were sequenced and analyzed. A hundred and sixty-eight genes were differentially expressed. The yeast gene expression patterns were found to be dependent on the severity of the cold with more genes being differentially expressed at − 12 °C than at 0 °C. Glaciozyma antarctica PI12 was found to share some common adaptation strategies with other yeasts, Saccharomyces cerevisiae and Mrakia spp., but at the same time, found to have some of its own unique strategies and mechanisms. Among the unique mechanisms was the production of antifreeze protein to prevent ice-crystallization inside and outside the cell. In addition, several molecular chaperones, detoxifiers of reactive oxygen species (ROS), and transcription and translation genes were constitutively expressed in G. antarctica PI12 to enable the cells to endure the fluctuating freezing temperatures. Interestingly, G. antarctica PI12 used nitrite as an alternative terminal acceptor of electrons when the oxygen level was low to minimize disruption of energy production in the cell. These mechanisms coupled with several other common mechanisms ensured that G. antarctica PI12 adapted well to the cold temperatures. Article in Journal/Newspaper Antarc* Antarctica Polar Biology Universiti Malaysia Sabah: UMS Institutional Repository |
institution |
Open Polar |
collection |
Universiti Malaysia Sabah: UMS Institutional Repository |
op_collection_id |
ftunivmalaysab |
language |
English |
topic |
QR Microbiology |
spellingShingle |
QR Microbiology Wong, Clemente Michael Vui Ling Sook, Yee Boo Voo, Christopher Lok Yung Nursyafiqi Zainuddin Nazalan Najimudin A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
topic_facet |
QR Microbiology |
description |
Glaciozyma antarctica PI12, a psychrophilic yeast from Antarctica, grows well at low temperatures. However, it is not clear how it responds and adapts to cold and freeze stresses. Hence, this project was set out to determine the cold-adaptation strategies and mechanisms of G. antarctica PI12 using a transcriptomic analysis approach. G. antarctica PI12 cells, grown in rich medium at 12 °C, were exposed to freeze stress at 0 and − 12 °C for 6 h and 24 h. Their transcriptomes were sequenced and analyzed. A hundred and sixty-eight genes were differentially expressed. The yeast gene expression patterns were found to be dependent on the severity of the cold with more genes being differentially expressed at − 12 °C than at 0 °C. Glaciozyma antarctica PI12 was found to share some common adaptation strategies with other yeasts, Saccharomyces cerevisiae and Mrakia spp., but at the same time, found to have some of its own unique strategies and mechanisms. Among the unique mechanisms was the production of antifreeze protein to prevent ice-crystallization inside and outside the cell. In addition, several molecular chaperones, detoxifiers of reactive oxygen species (ROS), and transcription and translation genes were constitutively expressed in G. antarctica PI12 to enable the cells to endure the fluctuating freezing temperatures. Interestingly, G. antarctica PI12 used nitrite as an alternative terminal acceptor of electrons when the oxygen level was low to minimize disruption of energy production in the cell. These mechanisms coupled with several other common mechanisms ensured that G. antarctica PI12 adapted well to the cold temperatures. |
format |
Article in Journal/Newspaper |
author |
Wong, Clemente Michael Vui Ling Sook, Yee Boo Voo, Christopher Lok Yung Nursyafiqi Zainuddin Nazalan Najimudin |
author_facet |
Wong, Clemente Michael Vui Ling Sook, Yee Boo Voo, Christopher Lok Yung Nursyafiqi Zainuddin Nazalan Najimudin |
author_sort |
Wong, Clemente Michael Vui Ling |
title |
A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
title_short |
A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
title_full |
A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
title_fullStr |
A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
title_full_unstemmed |
A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12 |
title_sort |
comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, glaciozyma antarctica pi12 |
publisher |
Springer Berlin Heidelberg |
publishDate |
2019 |
url |
http://eprints.ums.edu.my/id/eprint/27570/ http://eprints.ums.edu.my/id/eprint/27570/1/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20FULL%20TEXT.pdf http://eprints.ums.edu.my/id/eprint/27570/2/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20ABSTRACT.pdf https://link.springer.com/content/pdf/10.1007/s00300-018-02443-7.pdf |
genre |
Antarc* Antarctica Polar Biology |
genre_facet |
Antarc* Antarctica Polar Biology |
op_relation |
http://eprints.ums.edu.my/id/eprint/27570/1/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20FULL%20TEXT.pdf http://eprints.ums.edu.my/id/eprint/27570/2/A%20comparative%20transcriptomic%20analysis%20provides%20insights%20into%20the%20cold-adaptation%20mechanisms%20of%20a%20psychrophilic%20yeast,%20Glaciozyma%20antarctica%20PI12%20ABSTRACT.pdf Wong, Clemente Michael Vui Ling and Sook, Yee Boo and Voo, Christopher Lok Yung and Nursyafiqi Zainuddin and Nazalan Najimudin (2019) A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica PI12. Polar Biology, 42. pp. 541-553. ISSN 1432-2056 |
_version_ |
1766071564549226496 |