Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress
Psychrophilic yeast, Glaciozyma antarctica PI12 was isolated from the sea ice in the Southern Ocean. Several recent studies have revealed some of the strategies employed by G. antarctica PI12 to grow and survive at low temperatures, but those information are still limited. Hence this project was car...
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ftunivmalaysab:oai:eprints.ums.edu.my:25820 2023-05-15T13:35:53+02:00 Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress Koh, Joseph Soon Peng Vui Ling Wong, Clemente Michael Nazalan Najimudin Nor Muhammad Mahadi 2019 text http://eprints.ums.edu.my/id/eprint/25820/ http://eprints.ums.edu.my/id/eprint/25820/1/Gene%20expression%20patterns%20of%20Glaciozyma%20antarctica%20PI12%20in%20response%20to%20cold,%20and%20freeze%20stress.pdf https://doi.org/10.1016/j.polar.2018.11.007 en eng http://eprints.ums.edu.my/id/eprint/25820/1/Gene%20expression%20patterns%20of%20Glaciozyma%20antarctica%20PI12%20in%20response%20to%20cold,%20and%20freeze%20stress.pdf Koh, Joseph Soon Peng and Vui Ling Wong, Clemente Michael and Nazalan Najimudin and Nor Muhammad Mahadi (2019) Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress. Polar Science, 20 (1). pp. 45-54. Q Science (General) Article PeerReviewed 2019 ftunivmalaysab https://doi.org/10.1016/j.polar.2018.11.007 2022-03-29T15:41:30Z Psychrophilic yeast, Glaciozyma antarctica PI12 was isolated from the sea ice in the Southern Ocean. Several recent studies have revealed some of the strategies employed by G. antarctica PI12 to grow and survive at low temperatures, but those information are still limited. Hence this project was carried out to sequence the whole transcriptome to generate additional information on the cold-adaptation strategies of G. antarctica PI12. The yeast grown optimally in Saccharomyces cerevisiae minimal medium at 12 °C was subsequently exposed to cold-shock at 0 °C and freeze-shock at −12 °C for 6 h and 24 h. RNA from those cells were extracted, sequenced, and analyzed. Interestingly, the results showed that G. antarctica PI12 remained metabolically active at −12 °C. Two hundred and five genes were differentially expressed in the cells. Among them, 107 genes were upregulated while 98 genes were downregulated. In the first 6 h after the cells were exposed to cold- and freeze-shocks, CCR4-NOT (carbon catabolite repressed 4 - negative on TATA-less) core subunit cdc36, DNA repair protein Rad8, Elongation factor 1-gamma, 26s proteosome subunit 45, and Homocitrate synthase genes were commonly upregulated to perform several immediate and important tasks to ensure the cells survived. Apart from that, there were also genes upregulated and downregulated uniquely at 6 h and 24 h to facilitate the cells to adjust to the new temperatures. After overcoming the stress of the cold- and freeze-shocks for 24 h, the cells acclimatized to those temperatures and became metabolically active again, and the cell cycles related genes were up-regulated. Article in Journal/Newspaper Antarc* Antarctica Polar Science Polar Science Sea ice Southern Ocean Universiti Malaysia Sabah: UMS Institutional Repository Southern Ocean Polar Science 20 45 54 |
institution |
Open Polar |
collection |
Universiti Malaysia Sabah: UMS Institutional Repository |
op_collection_id |
ftunivmalaysab |
language |
English |
topic |
Q Science (General) |
spellingShingle |
Q Science (General) Koh, Joseph Soon Peng Vui Ling Wong, Clemente Michael Nazalan Najimudin Nor Muhammad Mahadi Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
topic_facet |
Q Science (General) |
description |
Psychrophilic yeast, Glaciozyma antarctica PI12 was isolated from the sea ice in the Southern Ocean. Several recent studies have revealed some of the strategies employed by G. antarctica PI12 to grow and survive at low temperatures, but those information are still limited. Hence this project was carried out to sequence the whole transcriptome to generate additional information on the cold-adaptation strategies of G. antarctica PI12. The yeast grown optimally in Saccharomyces cerevisiae minimal medium at 12 °C was subsequently exposed to cold-shock at 0 °C and freeze-shock at −12 °C for 6 h and 24 h. RNA from those cells were extracted, sequenced, and analyzed. Interestingly, the results showed that G. antarctica PI12 remained metabolically active at −12 °C. Two hundred and five genes were differentially expressed in the cells. Among them, 107 genes were upregulated while 98 genes were downregulated. In the first 6 h after the cells were exposed to cold- and freeze-shocks, CCR4-NOT (carbon catabolite repressed 4 - negative on TATA-less) core subunit cdc36, DNA repair protein Rad8, Elongation factor 1-gamma, 26s proteosome subunit 45, and Homocitrate synthase genes were commonly upregulated to perform several immediate and important tasks to ensure the cells survived. Apart from that, there were also genes upregulated and downregulated uniquely at 6 h and 24 h to facilitate the cells to adjust to the new temperatures. After overcoming the stress of the cold- and freeze-shocks for 24 h, the cells acclimatized to those temperatures and became metabolically active again, and the cell cycles related genes were up-regulated. |
format |
Article in Journal/Newspaper |
author |
Koh, Joseph Soon Peng Vui Ling Wong, Clemente Michael Nazalan Najimudin Nor Muhammad Mahadi |
author_facet |
Koh, Joseph Soon Peng Vui Ling Wong, Clemente Michael Nazalan Najimudin Nor Muhammad Mahadi |
author_sort |
Koh, Joseph Soon Peng |
title |
Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
title_short |
Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
title_full |
Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
title_fullStr |
Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
title_full_unstemmed |
Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress |
title_sort |
gene expression patterns of glaciozyma antarctica pi12 in response to cold, and freeze stress |
publishDate |
2019 |
url |
http://eprints.ums.edu.my/id/eprint/25820/ http://eprints.ums.edu.my/id/eprint/25820/1/Gene%20expression%20patterns%20of%20Glaciozyma%20antarctica%20PI12%20in%20response%20to%20cold,%20and%20freeze%20stress.pdf https://doi.org/10.1016/j.polar.2018.11.007 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica Polar Science Polar Science Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctica Polar Science Polar Science Sea ice Southern Ocean |
op_relation |
http://eprints.ums.edu.my/id/eprint/25820/1/Gene%20expression%20patterns%20of%20Glaciozyma%20antarctica%20PI12%20in%20response%20to%20cold,%20and%20freeze%20stress.pdf Koh, Joseph Soon Peng and Vui Ling Wong, Clemente Michael and Nazalan Najimudin and Nor Muhammad Mahadi (2019) Gene expression patterns of Glaciozyma antarctica PI12 in response to cold, and freeze stress. Polar Science, 20 (1). pp. 45-54. |
op_doi |
https://doi.org/10.1016/j.polar.2018.11.007 |
container_title |
Polar Science |
container_volume |
20 |
container_start_page |
45 |
op_container_end_page |
54 |
_version_ |
1766071557867700224 |