Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng
Quorum quenching (QQ) has been suggested as an alternative to antibiotic treatments for pathogenic infections as it does not affect the viability of the pathogen and imposes minimum selection pressure promoting drug resistance. During an ecological survey for quorum quenching (QQ) bacteria from soil...
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ftunivmalayasr:oai:studentsrepo.um.edu.my:12205 2023-05-15T13:04:02+02:00 Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng See-Too , Wah Seng 2020-01 application/pdf http://studentsrepo.um.edu.my/12205/ http://studentsrepo.um.edu.my/12205/2/See%2DToo_Wah_Seng.pdf http://studentsrepo.um.edu.my/12205/9/wah_seng_compressed.pdf unknown http://studentsrepo.um.edu.my/12205/2/See%2DToo_Wah_Seng.pdf http://studentsrepo.um.edu.my/12205/9/wah_seng_compressed.pdf See-Too , Wah Seng (2020) Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng. PhD thesis, Universiti Malaya. QH301 Biology QH426 Genetics Thesis NonPeerReviewed 2020 ftunivmalayasr 2023-01-24T18:30:00Z Quorum quenching (QQ) has been suggested as an alternative to antibiotic treatments for pathogenic infections as it does not affect the viability of the pathogen and imposes minimum selection pressure promoting drug resistance. During an ecological survey for quorum quenching (QQ) bacteria from soil collected in Lagoon Island (Ryder Bay, Adelaide Island, Antarctica), a bacterial strain was isolated and identified as Planococcus species, L10.15T, based on 16S rRNA gene analysis. The genomic, phylogenetic, chemotaxonomic and phenotypic data obtained showed that L10.15T represents a novel species for which the name Planococcus versutus sp. nov. is proposed. Previously, QQ activity of L10.15T was studied, and it was confirmed to be capable of inactivating synthetic N-acyl homoserine lactones (AHLs) with acyl side chain lengths C4-C12, and active at a temperature as low as 4 °C. In this study, the gene responsible for the QQ activity of P. versutus L10.15T was identified and confirmed for its function in a gene expression study. The cold-active characteristics of the enzyme coded by this gene suggested that it belonged to a novel class of N-acyl homoserine lactonase, and we therefore term the gene as ‘autoinducer degrading gene from Planococcus sp.’ (aidP). The aidP gene was then cloned, and its gene product AidP enzyme, which is a novel class of AHL lactonase from the metallo-ß-lactamase superfamily gene, was characterized. Multiple sequence alignment analysis and amino acid composition suggested that the aidP gene encoded a cold-adapted enzyme. Interestingly, the aidP gene has only been detected in Planococcus spp. that have been isolated from Antarctica. Therefore, branch-site analysis was conducted on several Planococcus spp. branches within the phylogenetic tree of homologous gene, showing evidence of episodic positive selection for the aidP gene in cold environments. The gene showed homology to several metallo-β-lactamase proteins obtained from Bacteroidetes but not to any known AHL-degrading enzymes. Liquid ... Thesis Adelaide Island Antarc* Antarctic Antarctica Lagoon Island University of Malaya: UM Students' Repository Adelaide Island ENVELOPE(-68.914,-68.914,-67.762,-67.762) Antarctic Lagoon Island ENVELOPE(-68.239,-68.239,-67.594,-67.594) Ryder ENVELOPE(-68.333,-68.333,-67.566,-67.566) Ryder Bay ENVELOPE(-68.333,-68.333,-67.567,-67.567) |
institution |
Open Polar |
collection |
University of Malaya: UM Students' Repository |
op_collection_id |
ftunivmalayasr |
language |
unknown |
topic |
QH301 Biology QH426 Genetics |
spellingShingle |
QH301 Biology QH426 Genetics See-Too , Wah Seng Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
topic_facet |
QH301 Biology QH426 Genetics |
description |
Quorum quenching (QQ) has been suggested as an alternative to antibiotic treatments for pathogenic infections as it does not affect the viability of the pathogen and imposes minimum selection pressure promoting drug resistance. During an ecological survey for quorum quenching (QQ) bacteria from soil collected in Lagoon Island (Ryder Bay, Adelaide Island, Antarctica), a bacterial strain was isolated and identified as Planococcus species, L10.15T, based on 16S rRNA gene analysis. The genomic, phylogenetic, chemotaxonomic and phenotypic data obtained showed that L10.15T represents a novel species for which the name Planococcus versutus sp. nov. is proposed. Previously, QQ activity of L10.15T was studied, and it was confirmed to be capable of inactivating synthetic N-acyl homoserine lactones (AHLs) with acyl side chain lengths C4-C12, and active at a temperature as low as 4 °C. In this study, the gene responsible for the QQ activity of P. versutus L10.15T was identified and confirmed for its function in a gene expression study. The cold-active characteristics of the enzyme coded by this gene suggested that it belonged to a novel class of N-acyl homoserine lactonase, and we therefore term the gene as ‘autoinducer degrading gene from Planococcus sp.’ (aidP). The aidP gene was then cloned, and its gene product AidP enzyme, which is a novel class of AHL lactonase from the metallo-ß-lactamase superfamily gene, was characterized. Multiple sequence alignment analysis and amino acid composition suggested that the aidP gene encoded a cold-adapted enzyme. Interestingly, the aidP gene has only been detected in Planococcus spp. that have been isolated from Antarctica. Therefore, branch-site analysis was conducted on several Planococcus spp. branches within the phylogenetic tree of homologous gene, showing evidence of episodic positive selection for the aidP gene in cold environments. The gene showed homology to several metallo-β-lactamase proteins obtained from Bacteroidetes but not to any known AHL-degrading enzymes. Liquid ... |
format |
Thesis |
author |
See-Too , Wah Seng |
author_facet |
See-Too , Wah Seng |
author_sort |
See-Too , Wah Seng |
title |
Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
title_short |
Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
title_full |
Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
title_fullStr |
Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
title_full_unstemmed |
Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng |
title_sort |
genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, planococcus versutus l10.15t / see-too wah seng |
publishDate |
2020 |
url |
http://studentsrepo.um.edu.my/12205/ http://studentsrepo.um.edu.my/12205/2/See%2DToo_Wah_Seng.pdf http://studentsrepo.um.edu.my/12205/9/wah_seng_compressed.pdf |
long_lat |
ENVELOPE(-68.914,-68.914,-67.762,-67.762) ENVELOPE(-68.239,-68.239,-67.594,-67.594) ENVELOPE(-68.333,-68.333,-67.566,-67.566) ENVELOPE(-68.333,-68.333,-67.567,-67.567) |
geographic |
Adelaide Island Antarctic Lagoon Island Ryder Ryder Bay |
geographic_facet |
Adelaide Island Antarctic Lagoon Island Ryder Ryder Bay |
genre |
Adelaide Island Antarc* Antarctic Antarctica Lagoon Island |
genre_facet |
Adelaide Island Antarc* Antarctic Antarctica Lagoon Island |
op_relation |
http://studentsrepo.um.edu.my/12205/2/See%2DToo_Wah_Seng.pdf http://studentsrepo.um.edu.my/12205/9/wah_seng_compressed.pdf See-Too , Wah Seng (2020) Genomic and taxonomic studies of an antarctic psychrotolerant quorum quenching bacterium, Planococcus versutus L10.15T / See-Too Wah Seng. PhD thesis, Universiti Malaya. |
_version_ |
1766350554213122048 |