Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia
Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different si...
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ftunivmalaya:oai:generic.eprints.org:9965 2023-05-15T18:05:17+02:00 Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia Benacer, D. Siti NurSheena, M.Z. Fairuz, A. Galloway, R.L. Thong, Kwai Lin 2013-07-03 application/pdf http://eprints.um.edu.my/9965/ http://eprints.um.edu.my/9965/1/Benacer,_2014.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856/ unknown NCBI http://eprints.um.edu.my/9965/1/Benacer,_2014.pdf Benacer, D.; Siti NurSheena, M.Z.; Fairuz, A.; Galloway, R.L.; Thong, Kwai Lin (2013) Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia <http://eprints.um.edu.my/view/publication/Isolation_and_Molecular_Characterization_of_Leptospira_interrogans_and_Leptospira_borgpetersenii_Isolates_from_the_Urban_Rat_Populations_of_Kuala_Lumpur,_Malaysia.html>, 88 (4). pp. 704-709. Q Science (General) General works Article PeerReviewed 2013 ftunivmalaya 2018-10-16T15:01:14Z Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different sites were trapped. Twenty cultures were positive for Leptospira using dark-field microscopy. R. rattus was the dominant carrier (70%). Polymerase chain reaction (PCR) confirmed that all isolates were pathogenic Leptospira species. Two Leptospira serogroups, Javanica and Bataviae, were identified using microscopic agglutination test (MAT). Pulsed-field gel electrophoresis (PFGE) identified two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (85%) and L. interrogans serovar Bataviae (15%). We conclude that these two serovars are the major serovars circulating among the urban rat populations in Kuala Lumpur. Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis. Article in Journal/Newspaper Rattus rattus University of Malaya: UM Institutional Repository |
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University of Malaya: UM Institutional Repository |
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Q Science (General) General works |
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Q Science (General) General works Benacer, D. Siti NurSheena, M.Z. Fairuz, A. Galloway, R.L. Thong, Kwai Lin Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
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Q Science (General) General works |
description |
Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different sites were trapped. Twenty cultures were positive for Leptospira using dark-field microscopy. R. rattus was the dominant carrier (70%). Polymerase chain reaction (PCR) confirmed that all isolates were pathogenic Leptospira species. Two Leptospira serogroups, Javanica and Bataviae, were identified using microscopic agglutination test (MAT). Pulsed-field gel electrophoresis (PFGE) identified two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (85%) and L. interrogans serovar Bataviae (15%). We conclude that these two serovars are the major serovars circulating among the urban rat populations in Kuala Lumpur. Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Benacer, D. Siti NurSheena, M.Z. Fairuz, A. Galloway, R.L. Thong, Kwai Lin |
author_facet |
Benacer, D. Siti NurSheena, M.Z. Fairuz, A. Galloway, R.L. Thong, Kwai Lin |
author_sort |
Benacer, D. |
title |
Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
title_short |
Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
title_full |
Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
title_fullStr |
Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
title_full_unstemmed |
Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia |
title_sort |
isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of kuala lumpur, malaysia |
publisher |
NCBI |
publishDate |
2013 |
url |
http://eprints.um.edu.my/9965/ http://eprints.um.edu.my/9965/1/Benacer,_2014.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617856/ |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://eprints.um.edu.my/9965/1/Benacer,_2014.pdf Benacer, D.; Siti NurSheena, M.Z.; Fairuz, A.; Galloway, R.L.; Thong, Kwai Lin (2013) Isolation and molecular characterization of leptospira interrogans and leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia <http://eprints.um.edu.my/view/publication/Isolation_and_Molecular_Characterization_of_Leptospira_interrogans_and_Leptospira_borgpetersenii_Isolates_from_the_Urban_Rat_Populations_of_Kuala_Lumpur,_Malaysia.html>, 88 (4). pp. 704-709. |
_version_ |
1766176744260239360 |