Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates
BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, tempera...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Society of Chemical Industry
2013
|
Subjects: | |
Online Access: | http://eprints.um.edu.my/9632/ http://eprints.um.edu.my/9632/1/00013161_103461.pdf |
id |
ftunivmalaya:oai:generic.eprints.org:9632 |
---|---|
record_format |
openpolar |
spelling |
ftunivmalaya:oai:generic.eprints.org:9632 2023-05-15T13:57:22+02:00 Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates Gumel, A.M. Annuar, M.S.M. Heidelberg, T. 2013 application/pdf http://eprints.um.edu.my/9632/ http://eprints.um.edu.my/9632/1/00013161_103461.pdf unknown Society of Chemical Industry http://eprints.um.edu.my/9632/1/00013161_103461.pdf Gumel, A.M.; Annuar, M.S.M.; Heidelberg, T. (2013) Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates. Journal of Chemical Technology and Biotechnology <http://eprints.um.edu.my/view/publication/Journal_of_Chemical_Technology_and_Biotechnology.html>. pp. 1328-1335. Q Science (General) QD Chemistry Article PeerReviewed 2013 ftunivmalaya 2015-12-23T16:04:47Z BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, temperature,enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1’-O-3-hydroxyacyl-sucrose) using Candida antarctica lipase B (EC 3.1.1.3)were studied. Using H2O2 as micro-initiator, enzyme-mediated synthesis yielded reaction rate, vapp of 0.076 x 10−5 mol L−1 s−1. The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non-functionalized material apart from showing better compostability. Increasing the reaction temperature (>50◦C), enzyme concentration (>15 g L−1) and reactant ratio (w/w) of sucrose:PHA (>2) did not increase further the rate or yield. The sucrose-functionalized mcl-PHAwas characterized with respect to the non-functionalized material. CONCLUSIONS: Novozym 435 can be used effectively to synthesize poly(1’-O-3-hydroxyacyl sucrose) in micro-aqueous medium bypassing the need for chemo-synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches Article in Journal/Newspaper Antarc* Antarctica University of Malaya: UM Institutional Repository |
institution |
Open Polar |
collection |
University of Malaya: UM Institutional Repository |
op_collection_id |
ftunivmalaya |
language |
unknown |
topic |
Q Science (General) QD Chemistry |
spellingShingle |
Q Science (General) QD Chemistry Gumel, A.M. Annuar, M.S.M. Heidelberg, T. Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
topic_facet |
Q Science (General) QD Chemistry |
description |
BACKGROUND: Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates (PHA) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial. RESULTS:Theeffects of reaction variables reaction time, temperature,enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1’-O-3-hydroxyacyl-sucrose) using Candida antarctica lipase B (EC 3.1.1.3)were studied. Using H2O2 as micro-initiator, enzyme-mediated synthesis yielded reaction rate, vapp of 0.076 x 10−5 mol L−1 s−1. The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non-functionalized material apart from showing better compostability. Increasing the reaction temperature (>50◦C), enzyme concentration (>15 g L−1) and reactant ratio (w/w) of sucrose:PHA (>2) did not increase further the rate or yield. The sucrose-functionalized mcl-PHAwas characterized with respect to the non-functionalized material. CONCLUSIONS: Novozym 435 can be used effectively to synthesize poly(1’-O-3-hydroxyacyl sucrose) in micro-aqueous medium bypassing the need for chemo-synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches |
format |
Article in Journal/Newspaper |
author |
Gumel, A.M. Annuar, M.S.M. Heidelberg, T. |
author_facet |
Gumel, A.M. Annuar, M.S.M. Heidelberg, T. |
author_sort |
Gumel, A.M. |
title |
Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
title_short |
Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
title_full |
Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
title_fullStr |
Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
title_full_unstemmed |
Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
title_sort |
single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates |
publisher |
Society of Chemical Industry |
publishDate |
2013 |
url |
http://eprints.um.edu.my/9632/ http://eprints.um.edu.my/9632/1/00013161_103461.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://eprints.um.edu.my/9632/1/00013161_103461.pdf Gumel, A.M.; Annuar, M.S.M.; Heidelberg, T. (2013) Single-step lipase-catalyzed functionalization of medium-chain-length polyhydroxyalkanoates. Journal of Chemical Technology and Biotechnology <http://eprints.um.edu.my/view/publication/Journal_of_Chemical_Technology_and_Biotechnology.html>. pp. 1328-1335. |
_version_ |
1766265055490342912 |