Molecular diversity of bacteria in Antarctic and Arctic soils
The diversity of bacterial and actinobacterial community in soil samples from Signy Station, Antarctica and Dasan Station, Arctic was studied by PCR amplification and Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rRNA gene fragments. Non-metric multidimensional scaling plots (MDS) analysis o...
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ftunivmalaya:oai:generic.eprints.org:15085 2023-05-15T13:57:23+02:00 Molecular diversity of bacteria in Antarctic and Arctic soils Chin, L.K. Tan, G.Y.A. Tan, I.K.P. 2007 application/pdf http://eprints.um.edu.my/15085/ http://eprints.um.edu.my/15085/1/0001.pdf unknown http://eprints.um.edu.my/15085/1/0001.pdf Chin, L.K.; Tan, G.Y.A.; Tan, I.K.P. (2007) Molecular diversity of bacteria in Antarctic and Arctic soils. In: The 12th Biological Sciences Graduate Congress 2007, 17-19 December 2007, Institute of Biological Sciences, Faculty of Science, University of Malaya. Q Science (General) QH Natural history Conference or Workshop Item PeerReviewed 2007 ftunivmalaya 2015-12-23T16:08:55Z The diversity of bacterial and actinobacterial community in soil samples from Signy Station, Antarctica and Dasan Station, Arctic was studied by PCR amplification and Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rRNA gene fragments. Non-metric multidimensional scaling plots (MDS) analysis of DGGE profiles suggested that the Antarctica and Arctic soils used in this study have around 20% similarity of bacterial diversity. Major DGGE bands were excised from the gels, re-amplified and sequenced. The main bacterial groups in the Antarctica and Arctic soils fell into the Bacteriodetes, Actinobacteria and Gemmatimonadetes. There were several sequences which were not related to any known bacteria group. In addition, the presence of actinobacteria from the family Micromonosporineae, Frankineae, Micrococcineae, Streptosporangineae and Pseudonocardineae was detected in the Antarctica and Arctic soils. These results showed the presence of a diverse community of bacteria and actinobacteria in soils collected from the Signy Station, Antarctic and Dasan Station, Arctic. The correlation of environmental variables including soil water content, pH, electrical conductivity, carbon, hydrogen and nitrogen on the bacterial and actinobacterial community in this study is also presented. Conference Object Antarc* Antarctic Antarctica Arctic Arctic University of Malaya: UM Institutional Repository Antarctic Arctic Signy Station ENVELOPE(-45.600,-45.600,-60.717,-60.717) |
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University of Malaya: UM Institutional Repository |
op_collection_id |
ftunivmalaya |
language |
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topic |
Q Science (General) QH Natural history |
spellingShingle |
Q Science (General) QH Natural history Chin, L.K. Tan, G.Y.A. Tan, I.K.P. Molecular diversity of bacteria in Antarctic and Arctic soils |
topic_facet |
Q Science (General) QH Natural history |
description |
The diversity of bacterial and actinobacterial community in soil samples from Signy Station, Antarctica and Dasan Station, Arctic was studied by PCR amplification and Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rRNA gene fragments. Non-metric multidimensional scaling plots (MDS) analysis of DGGE profiles suggested that the Antarctica and Arctic soils used in this study have around 20% similarity of bacterial diversity. Major DGGE bands were excised from the gels, re-amplified and sequenced. The main bacterial groups in the Antarctica and Arctic soils fell into the Bacteriodetes, Actinobacteria and Gemmatimonadetes. There were several sequences which were not related to any known bacteria group. In addition, the presence of actinobacteria from the family Micromonosporineae, Frankineae, Micrococcineae, Streptosporangineae and Pseudonocardineae was detected in the Antarctica and Arctic soils. These results showed the presence of a diverse community of bacteria and actinobacteria in soils collected from the Signy Station, Antarctic and Dasan Station, Arctic. The correlation of environmental variables including soil water content, pH, electrical conductivity, carbon, hydrogen and nitrogen on the bacterial and actinobacterial community in this study is also presented. |
format |
Conference Object |
author |
Chin, L.K. Tan, G.Y.A. Tan, I.K.P. |
author_facet |
Chin, L.K. Tan, G.Y.A. Tan, I.K.P. |
author_sort |
Chin, L.K. |
title |
Molecular diversity of bacteria in Antarctic and Arctic soils |
title_short |
Molecular diversity of bacteria in Antarctic and Arctic soils |
title_full |
Molecular diversity of bacteria in Antarctic and Arctic soils |
title_fullStr |
Molecular diversity of bacteria in Antarctic and Arctic soils |
title_full_unstemmed |
Molecular diversity of bacteria in Antarctic and Arctic soils |
title_sort |
molecular diversity of bacteria in antarctic and arctic soils |
publishDate |
2007 |
url |
http://eprints.um.edu.my/15085/ http://eprints.um.edu.my/15085/1/0001.pdf |
long_lat |
ENVELOPE(-45.600,-45.600,-60.717,-60.717) |
geographic |
Antarctic Arctic Signy Station |
geographic_facet |
Antarctic Arctic Signy Station |
genre |
Antarc* Antarctic Antarctica Arctic Arctic |
genre_facet |
Antarc* Antarctic Antarctica Arctic Arctic |
op_relation |
http://eprints.um.edu.my/15085/1/0001.pdf Chin, L.K.; Tan, G.Y.A.; Tan, I.K.P. (2007) Molecular diversity of bacteria in Antarctic and Arctic soils. In: The 12th Biological Sciences Graduate Congress 2007, 17-19 December 2007, Institute of Biological Sciences, Faculty of Science, University of Malaya. |
_version_ |
1766265057456422912 |